FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_66.3410000000b119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_66.3410000000b119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546689
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC16360.29925606697775153RNA PCR Primer, Index 20 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7850.14359169472954458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.11286124286385862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1600.036.56175640
TGCCGTC1600.036.56175645
TGTTCGG250.002106353935.9992722
ATGCCGT1700.035.7345744
TATGCCG1750.034.7135843
CCCACGA1750.033.4278919
ACGAGAC1750.033.4278922
CATCTCG1900.031.97303436
ATCTCGT1900.030.78884737
CTGTGGA1850.030.40478729
GTCCTAT300.005122462830.0268551
CGTATGC1950.029.9993941
CTCGTAT1950.029.9993939
AGCCCAC1950.029.9993917
CCACGAG2000.029.24940720
CACGAGA2150.028.25524121
GAGACCT2000.028.1244324
CCGAGCC2100.027.85657714
CGAGCCC2100.027.85657715
TGTGGAC2050.027.43846730
Produced by FastQC (version 0.11.2)