FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_65.3410000000b10c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_65.3410000000b10c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983859
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC12420.12623760111967264No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGTA253.890965E-544.99888228
TGCCGTC1150.039.1294645
CCGCGTC651.4035322E-831.1530722
GCCCACG1450.031.03371218
CTCCGCG602.4189103E-729.99925420
CGAGCCC1550.029.03153815
CGTATGC1600.028.12430241
CCCACGA1600.028.12430219
CACTACG406.166402E-428.12430231
CCGAGCC1600.028.12430214
ATGCCGT1600.028.12430244
TCCGCGT654.8732545E-727.69161821
CGCGTCT654.8732545E-727.69161823
CCGTTAC1400.027.351331
AGGTACG755.638867E-826.9993320
CGCGGAG507.158578E-526.9993329
GTCTCGG507.158578E-526.9993312
TATGCCG1700.026.4699343
CCACGAG1700.026.4699320
GAGCCCA1750.025.71364616
Produced by FastQC (version 0.11.2)