FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_64.3410000000b0fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_64.3410000000b0fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195599
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC68390.5720145299552776No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCTGTCTCTT15530.12989304942543445No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT14590.12203088159157041No Hit
GTATAGATTCTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAAT12320.10304458267362218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9050.038.28618645
GCCCACG9500.036.7094718
CCACGAG9500.036.47263320
CCCACGA9700.035.95257619
TATGCCG9800.035.1265443
ACGAGAC10000.034.87399722
AGCCCAC9950.034.8231217
CGAGCCC9900.034.77172515
ATGCCGT9850.034.7198144
CCGAGCC10150.033.9152814
CTCGTAT10300.033.42136839
GAGCCCA10300.033.42136816
TCCGAGC10400.033.10000613
CGAGACC10450.032.9416323
CATCTCG10600.032.68773736
CACGAGA10800.032.49906521
CTCCGAG10750.032.2316312
ATCTCGT10900.031.78807437
GAGACCA10900.031.78807424
TCTCGTA10950.031.64292338
Produced by FastQC (version 0.11.2)