##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_4_62.3410000000b0d4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.93478260869565 0.0 0.0 0.0 0.0 0.0 2 36.47826086956522 0.0 0.0 0.0 0.0 0.0 3 36.69565217391305 0.0 0.0 0.0 0.0 0.0 4 35.869565217391305 0.0 0.0 0.0 0.0 0.0 5 35.65217391304348 0.0 0.0 0.0 0.0 0.0 6 37.130434782608695 0.0 0.0 0.0 0.0 0.0 7 37.369565217391305 0.0 0.0 0.0 0.0 0.0 8 37.608695652173914 0.0 0.0 0.0 0.0 0.0 9 36.391304347826086 0.0 0.0 0.0 0.0 0.0 10 36.34782608695652 0.0 0.0 0.0 0.0 0.0 11 35.608695652173914 0.0 0.0 0.0 0.0 0.0 12 36.15217391304348 0.0 0.0 0.0 0.0 0.0 13 36.30434782608695 0.0 0.0 0.0 0.0 0.0 14 36.47826086956522 0.0 0.0 0.0 0.0 0.0 15 36.08695652173913 0.0 0.0 0.0 0.0 0.0 16 37.06521739130435 0.0 0.0 0.0 0.0 0.0 17 36.65217391304348 0.0 0.0 0.0 0.0 0.0 18 37.391304347826086 0.0 0.0 0.0 0.0 0.0 19 37.84782608695652 0.0 0.0 0.0 0.0 0.0 20 37.54347826086956 0.0 0.0 0.0 0.0 0.0 21 37.32608695652174 0.0 0.0 0.0 0.0 0.0 22 37.108695652173914 0.0 0.0 0.0 0.0 0.0 23 37.41304347826087 0.0 0.0 0.0 0.0 0.0 24 36.91304347826087 0.0 0.0 0.0 0.0 0.0 25 36.130434782608695 0.0 0.0 0.0 0.0 0.0 26 35.869565217391305 0.0 0.0 0.0 0.0 0.0 27 36.54347826086956 0.0 0.0 0.0 0.0 0.0 28 35.82608695652174 0.0 0.0 0.0 0.0 0.0 29 35.82608695652174 0.0 0.0 0.0 0.0 0.0 30 35.19565217391305 0.0 0.0 0.0 0.0 0.0 31 35.67391304347826 0.0 0.0 0.0 0.0 0.0 32 37.630434782608695 0.0 0.0 0.0 0.0 0.0 33 36.30434782608695 0.0 0.0 0.0 0.0 0.0 34 37.04347826086956 0.0 0.0 0.0 0.0 0.0 35 36.0 0.0 0.0 0.0 0.0 0.0 36 36.21739130434783 0.0 0.0 0.0 0.0 0.0 37 35.869565217391305 0.0 0.0 0.0 0.0 0.0 38 35.41304347826087 0.0 0.0 0.0 0.0 0.0 39 35.5 0.0 0.0 0.0 0.0 0.0 40 35.73913043478261 0.0 0.0 0.0 0.0 0.0 41 36.06521739130435 0.0 0.0 0.0 0.0 0.0 42 35.34782608695652 0.0 0.0 0.0 0.0 0.0 43 35.91304347826087 0.0 0.0 0.0 0.0 0.0 44 35.19565217391305 0.0 0.0 0.0 0.0 0.0 45 35.391304347826086 0.0 0.0 0.0 0.0 0.0 46 36.30434782608695 0.0 0.0 0.0 0.0 0.0 47 36.95652173913044 0.0 0.0 0.0 0.0 0.0 48 36.69565217391305 0.0 0.0 0.0 0.0 0.0 49 35.15217391304348 0.0 0.0 0.0 0.0 0.0 50 35.91304347826087 0.0 0.0 0.0 0.0 0.0 51 34.91304347826087 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2203 1 0.0 2203 2 1.5 2203 3 -4.5 2203 4 NaN 2203 5 1.5 2203 6 -1.0 2203 7 0.5 2203 8 -4.0 2203 9 -2.0 2203 10 NaN 2203 11 NaN 2203 12 NaN 2203 13 NaN 2203 14 NaN 2203 15 NaN 2203 16 NaN 2203 17 NaN 2203 18 -3.0 2203 19 NaN 2203 20 NaN 2203 21 NaN 2203 22 NaN 2203 23 NaN 2203 24 NaN 2203 25 NaN 2203 26 NaN 2203 27 NaN 2203 28 NaN 2203 29 NaN 2203 30 NaN 2203 31 NaN 2203 32 NaN 2203 33 NaN 2203 34 NaN 2203 35 NaN 2203 36 NaN 2203 37 NaN 2203 38 NaN 2203 39 NaN 2203 40 NaN 2203 41 NaN 2203 42 NaN 2203 43 NaN 2203 44 NaN 2203 45 NaN 2203 46 NaN 2203 47 NaN 2203 48 NaN 2203 49 NaN 2203 50 NaN 2203 51 NaN 1204 1 0.0 1204 2 1.5 1204 3 1.5 1204 4 NaN 1204 5 1.5 1204 6 1.0 1204 7 2.5 1204 8 4.0 1204 9 -2.0 1204 10 NaN 1204 11 NaN 1204 12 NaN 1204 13 NaN 1204 14 NaN 1204 15 NaN 1204 16 NaN 1204 17 NaN 1204 18 3.0 1204 19 NaN 1204 20 NaN 1204 21 NaN 1204 22 NaN 1204 23 NaN 1204 24 NaN 1204 25 NaN 1204 26 NaN 1204 27 NaN 1204 28 NaN 1204 29 NaN 1204 30 NaN 1204 31 NaN 1204 32 NaN 1204 33 NaN 1204 34 NaN 1204 35 NaN 1204 36 NaN 1204 37 NaN 1204 38 NaN 1204 39 NaN 1204 40 NaN 1204 41 NaN 1204 42 NaN 1204 43 NaN 1204 44 NaN 1204 45 NaN 1204 46 NaN 1204 47 NaN 1204 48 NaN 1204 49 NaN 1204 50 NaN 1204 51 NaN 2106 1 0.0 2106 2 1.5 2106 3 1.5 2106 4 NaN 2106 5 1.5 2106 6 1.0 2106 7 -5.5 2106 8 -4.0 2106 9 -2.0 2106 10 NaN 2106 11 NaN 2106 12 NaN 2106 13 NaN 2106 14 NaN 2106 15 NaN 2106 16 NaN 2106 17 NaN 2106 18 -3.0 2106 19 NaN 2106 20 NaN 2106 21 NaN 2106 22 NaN 2106 23 NaN 2106 24 NaN 2106 25 NaN 2106 26 NaN 2106 27 NaN 2106 28 NaN 2106 29 NaN 2106 30 NaN 2106 31 NaN 2106 32 NaN 2106 33 NaN 2106 34 NaN 2106 35 NaN 2106 36 NaN 2106 37 NaN 2106 38 NaN 2106 39 NaN 2106 40 NaN 2106 41 NaN 2106 42 NaN 2106 43 NaN 2106 44 NaN 2106 45 NaN 2106 46 NaN 2106 47 NaN 2106 48 NaN 2106 49 NaN 2106 50 NaN 2106 51 NaN 1214 1 0.0 1214 2 -4.5 1214 3 1.5 1214 4 NaN 1214 5 -4.5 1214 6 -1.0 1214 7 2.5 1214 8 4.0 1214 9 6.0 1214 10 NaN 1214 11 NaN 1214 12 NaN 1214 13 NaN 1214 14 NaN 1214 15 NaN 1214 16 NaN 1214 17 NaN 1214 18 3.0 1214 19 NaN 1214 20 NaN 1214 21 NaN 1214 22 NaN 1214 23 NaN 1214 24 NaN 1214 25 NaN 1214 26 NaN 1214 27 NaN 1214 28 NaN 1214 29 NaN 1214 30 NaN 1214 31 NaN 1214 32 NaN 1214 33 NaN 1214 34 NaN 1214 35 NaN 1214 36 NaN 1214 37 NaN 1214 38 NaN 1214 39 NaN 1214 40 NaN 1214 41 NaN 1214 42 NaN 1214 43 NaN 1214 44 NaN 1214 45 NaN 1214 46 NaN 1214 47 NaN 1214 48 NaN 1214 49 NaN 1214 50 NaN 1214 51 NaN >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 2.0 27 1.0 28 2.0 29 0.0 30 1.0 31 1.0 32 2.0 33 1.0 34 2.0 35 1.0 36 6.0 37 5.0 38 9.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.78260869565217 19.565217391304348 21.73913043478261 23.91304347826087 2 4.3478260869565215 19.565217391304348 47.82608695652174 28.26086956521739 3 8.695652173913043 45.65217391304348 30.434782608695656 15.217391304347828 4 13.043478260869565 17.391304347826086 39.130434782608695 30.434782608695656 5 10.869565217391305 34.78260869565217 47.82608695652174 6.521739130434782 6 19.565217391304348 45.65217391304348 26.08695652173913 8.695652173913043 7 28.26086956521739 26.08695652173913 39.130434782608695 6.521739130434782 8 17.391304347826086 39.130434782608695 19.565217391304348 23.91304347826087 9 23.91304347826087 15.217391304347828 26.08695652173913 34.78260869565217 10 28.26086956521739 32.608695652173914 26.08695652173913 13.043478260869565 11 28.26086956521739 30.434782608695656 30.434782608695656 10.869565217391305 12 6.521739130434782 28.26086956521739 47.82608695652174 17.391304347826086 13 26.08695652173913 15.217391304347828 43.47826086956522 15.217391304347828 14 13.043478260869565 21.73913043478261 36.95652173913043 28.26086956521739 15 21.73913043478261 30.434782608695656 28.26086956521739 19.565217391304348 16 23.91304347826087 28.26086956521739 32.608695652173914 15.217391304347828 17 23.91304347826087 23.91304347826087 28.26086956521739 23.91304347826087 18 28.26086956521739 19.565217391304348 34.78260869565217 17.391304347826086 19 13.043478260869565 28.26086956521739 34.78260869565217 23.91304347826087 20 17.391304347826086 43.47826086956522 26.08695652173913 13.043478260869565 21 23.91304347826087 30.434782608695656 34.78260869565217 10.869565217391305 22 8.695652173913043 41.30434782608695 26.08695652173913 23.91304347826087 23 26.08695652173913 30.434782608695656 21.73913043478261 21.73913043478261 24 19.565217391304348 45.65217391304348 26.08695652173913 8.695652173913043 25 15.217391304347828 21.73913043478261 34.78260869565217 28.26086956521739 26 17.391304347826086 19.565217391304348 52.17391304347826 10.869565217391305 27 23.91304347826087 32.608695652173914 19.565217391304348 23.91304347826087 28 21.73913043478261 26.08695652173913 39.130434782608695 13.043478260869565 29 30.434782608695656 13.043478260869565 41.30434782608695 15.217391304347828 30 23.91304347826087 32.608695652173914 23.91304347826087 19.565217391304348 31 15.217391304347828 32.608695652173914 28.26086956521739 23.91304347826087 32 17.391304347826086 32.608695652173914 32.608695652173914 17.391304347826086 33 36.95652173913043 26.08695652173913 17.391304347826086 19.565217391304348 34 21.73913043478261 36.95652173913043 26.08695652173913 15.217391304347828 35 15.217391304347828 41.30434782608695 21.73913043478261 21.73913043478261 36 26.08695652173913 30.434782608695656 23.91304347826087 19.565217391304348 37 21.73913043478261 26.08695652173913 32.608695652173914 19.565217391304348 38 19.565217391304348 26.08695652173913 41.30434782608695 13.043478260869565 39 23.91304347826087 19.565217391304348 23.91304347826087 32.608695652173914 40 19.565217391304348 26.08695652173913 41.30434782608695 13.043478260869565 41 17.391304347826086 32.608695652173914 30.434782608695656 19.565217391304348 42 10.869565217391305 39.130434782608695 39.130434782608695 10.869565217391305 43 13.043478260869565 21.73913043478261 43.47826086956522 21.73913043478261 44 17.391304347826086 13.043478260869565 43.47826086956522 26.08695652173913 45 23.91304347826087 13.043478260869565 45.65217391304348 17.391304347826086 46 19.565217391304348 34.78260869565217 32.608695652173914 13.043478260869565 47 15.217391304347828 21.73913043478261 28.26086956521739 34.78260869565217 48 15.217391304347828 19.565217391304348 43.47826086956522 21.73913043478261 49 17.391304347826086 15.217391304347828 32.608695652173914 34.78260869565217 50 8.695652173913043 21.73913043478261 41.30434782608695 28.26086956521739 51 10.869565217391305 23.91304347826087 39.130434782608695 26.08695652173913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 1.5 31 3.0 32 3.0 33 3.0 34 7.5 35 12.0 36 9.5 37 7.0 38 7.0 39 7.0 40 6.0 41 5.0 42 3.0 43 1.0 44 1.5 45 2.0 46 1.0 47 0.0 48 1.5 49 3.0 50 2.5 51 2.0 52 1.0 53 0.0 54 0.5 55 1.0 56 0.5 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 46.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTCCTTGCAGCTCTAAAAATCGGTACATCTTGTACATCTGTCTCTTATAC 1 2.1739130434782608 No Hit CCTCATTATAGTCTTCCTCATGCATGAATATAGTACCAGCAATCAACCCTG 1 2.1739130434782608 No Hit CTCAGATTCTATTGTCTACAATCTCATCCACACCAATCTTAAATGTTTACG 1 2.1739130434782608 No Hit TTATTAGGCCATGCAATTACTCAAGTAGCAAAAAAATGTAGTACTGTCTCT 1 2.1739130434782608 No Hit GAAGAATATGAATGGAATTGGTGTTTTTTTCCTGTCTCTTATACACATCTC 1 2.1739130434782608 No Hit CAAATTTTGAGAAAGCTTATGAAACCGTTGAAGAGAAGTTTCTTAGGTTCG 1 2.1739130434782608 No Hit TCACTGTATTGTTCAGATGCATGTCTGAAGAGATATCTGGAAGCTAGGAAT 1 2.1739130434782608 No Hit CCACTATAGGTATACCGAGAGAGGCTGCCGCCATGGCACCCTTATACCCAA 1 2.1739130434782608 No Hit GACTTTTCTTGTGTCTTCTATTTTCTGGACTCAAGAGTAAAAACTGTCTCT 1 2.1739130434782608 No Hit TTCTTAATCAGAGTGTCTTCACAAGTCTTTCTGTAATCAGTCTGTCTCTTA 1 2.1739130434782608 No Hit GTACGGGGGATAACAGGTTGATATTCCTGTACCACCTATAATCTGTCTCTT 1 2.1739130434782608 No Hit GATATAGCTGGTTTTCCGCGAAATCTATTTCAGTAGAGCGTATGATGTCTG 1 2.1739130434782608 No Hit GTATGTGAAGAAGAGAATTAGATAGCCTGATTTCAATCAACAAGCAAGTGT 1 2.1739130434782608 No Hit CGTTTAATCATCGTTATGTTAAATCTAATCCACGCGAAGTAGAGAACGCTA 1 2.1739130434782608 No Hit ATATTTGGTAATGCTGCTATGAAACTGTTAGTGATTGTAAGACTGCTTGCT 1 2.1739130434782608 No Hit GCAGTCACCTTCTTATCCTTACTCTTAAGAGCATCAAGAGTCGCTACTTCC 1 2.1739130434782608 No Hit ACTATAAGGATCTGAAAGTAGCGAAATTCCTTGGCCATTAAATGTGGTCCT 1 2.1739130434782608 No Hit AAAGTATTCCGATTCTGCAAGTCGAAATGCCATAAGAACTTTAAGATCTGT 1 2.1739130434782608 No Hit AGTGAAGATCATCATTCAGATCCAGACTCTTGACTGTTGTGCTTTTATTAA 1 2.1739130434782608 No Hit GTTGTTTGGGATCCATTCCACAAAGTAGGATGAGTTCTTGTTCTGAATGTT 1 2.1739130434782608 No Hit GTCTCAACCGTAATATCCCAATCAATGCTGTCTGCAGCCGCATCTGTACCA 1 2.1739130434782608 No Hit CTGCTTAGATAACCGTTGTTTTGGTTTATGAGGAGCCACTGTTTTAATATC 1 2.1739130434782608 No Hit CTTCACTACGATAAGAGGAATCTTTTTGGGAAGTAGATCCCTGTCTCTTAT 1 2.1739130434782608 No Hit GTAATGGACAGTTTGGATCACCGACTAGTAATGGATTTGGATCTGTCTCTT 1 2.1739130434782608 No Hit TCATATCTTTCTTACTGCGTAACAATATTTACCAAGGGTAATTTATCTGTC 1 2.1739130434782608 No Hit GTCTTGTTTATATCTTCCCAATTCTTTATATGATCTGACAAATCTCCTCTA 1 2.1739130434782608 No Hit TCTTATACACATCTCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC 1 2.1739130434782608 No Hit CTACGAGAAGGATACAGGTTTATACGTTCTGAAGCTGTCTCTTATACACAT 1 2.1739130434782608 No Hit GTGTACGCCCAAGCAGTGAGTCGGCTGATGAGTCAAATGCCTGTCTCTTAT 1 2.1739130434782608 No Hit AAGAAGAAGAATAAGAAGAAGAGAAAGCATGAGGAAGAAGAACTGTCTCTT 1 2.1739130434782608 No Hit TTATTATGCTTTAACACTAAATCTTGATGATTCCCGTACTCTGCGTTGATA 1 2.1739130434782608 No Hit GCAAAAGCATTGCCTTTCTCGTAGGAATGGATTCTTTGGGGACGTAAACGT 1 2.1739130434782608 No Hit CTCACATATTCCTTAGATTTGAAACTCTGCATCAACTTGATAACTTGCAGA 1 2.1739130434782608 No Hit ATGTATGAGACGCCAAGAGAACTGTACAGAAAGATTAGGCTATCGAGAACA 1 2.1739130434782608 No Hit GAATGGAAACAATTGCGAAACAGTTTGGTATTAAGCCTGTCTCTTATACAC 1 2.1739130434782608 No Hit TCTCAGTACACTTCTTTTCATACCTCAAGAGACTAAAACTAGTTTGAGGAG 1 2.1739130434782608 No Hit TCTCTGTCCTTCTTAAAGTTGAAAAGTCTTTTGAAGTAATTTTTGAAGGCA 1 2.1739130434782608 No Hit TATTTGCTGGGTGTTTTTCTTGATTGCTGAGGTATGTTCTGTCTCTTATAC 1 2.1739130434782608 No Hit ACACAAAAGGGTGGTGGTACTCTAATTGCAATGGTATTGATACTTCAAATT 1 2.1739130434782608 No Hit TCTCAAACTGTTGTGGTATGTGGAAACTGTCAGACAGTTCTGTGCCAGCCC 1 2.1739130434782608 No Hit GTTCATGCTGCTTGCGCGTTAGCATTCGTGCGCACTCCTATCTGTATCTTA 1 2.1739130434782608 No Hit CTATACATCGTGTTACCACTTAGCAGAGTCCTGTGTTTTTAATCTGTCTCT 1 2.1739130434782608 No Hit GTAGTAGACATTGAAGAAGATAAACCCGAGTTACTGGAATAAGTCTGTCTC 1 2.1739130434782608 No Hit ACATTATCATTCGCTTGTTGTATTGTGAATCTAAACCGCTGTATGTCTGTC 1 2.1739130434782608 No Hit GTATTATAGTACTTCTGGAGCTAGTGATACAACTGTCTCTTATACACATCT 1 2.1739130434782608 No Hit CACCAACAGAGTAGAATGAGAATATTGAGGAAGGAAGGAATGAATGCTGTC 1 2.1739130434782608 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 2.1739130434782608 33 0.0 0.0 4.3478260869565215 34 0.0 0.0 4.3478260869565215 35 0.0 0.0 6.521739130434782 36 0.0 0.0 6.521739130434782 37 0.0 0.0 8.695652173913043 38 0.0 0.0 8.695652173913043 39 0.0 0.0 13.043478260869565 >>END_MODULE >>Kmer Content pass >>END_MODULE