##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_4_61.3410000000b0ba.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 141868 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.896629261003184 38.0 38.0 38.0 32.0 38.0 2 36.87111258352835 38.0 38.0 38.0 32.0 38.0 3 36.85929878478586 38.0 38.0 38.0 32.0 38.0 4 36.923245552203454 38.0 38.0 38.0 32.0 38.0 5 36.93993712465108 38.0 38.0 38.0 32.0 38.0 6 38.90194406067612 40.0 38.0 40.0 38.0 40.0 7 38.839336566385654 40.0 38.0 40.0 38.0 40.0 8 38.7813389911749 40.0 38.0 40.0 38.0 40.0 9 38.81103561056757 40.0 38.0 40.0 38.0 40.0 10 38.795549383934365 40.0 38.0 40.0 38.0 40.0 11 38.802478360165786 40.0 38.0 40.0 38.0 40.0 12 38.78603349592579 40.0 38.0 40.0 38.0 40.0 13 38.794513209462316 40.0 38.0 40.0 38.0 40.0 14 38.77108297854343 40.0 38.0 40.0 38.0 40.0 15 38.78159274818846 40.0 38.0 40.0 38.0 40.0 16 38.76903882482308 40.0 38.0 40.0 38.0 40.0 17 38.74123833422618 40.0 38.0 40.0 38.0 40.0 18 38.77128034510954 40.0 38.0 40.0 38.0 40.0 19 38.80016635182 40.0 38.0 40.0 38.0 40.0 20 38.7615318465052 40.0 38.0 40.0 38.0 40.0 21 38.80452251388615 40.0 38.0 40.0 38.0 40.0 22 38.799524910480166 40.0 38.0 40.0 38.0 40.0 23 38.802802605238675 40.0 38.0 40.0 38.0 40.0 24 38.79178532156653 40.0 38.0 40.0 38.0 40.0 25 38.762476386500126 40.0 38.0 40.0 38.0 40.0 26 38.70933543857671 40.0 38.0 40.0 38.0 40.0 27 38.680942848281504 40.0 38.0 40.0 38.0 40.0 28 38.62788648602927 40.0 38.0 40.0 38.0 40.0 29 38.56848619843799 40.0 38.0 40.0 38.0 40.0 30 38.51072123382299 40.0 38.0 40.0 38.0 40.0 31 38.409133842727044 40.0 38.0 40.0 38.0 40.0 32 38.30337355851918 40.0 38.0 40.0 38.0 40.0 33 38.15250091634477 40.0 38.0 40.0 38.0 40.0 34 38.01433727126625 40.0 38.0 40.0 38.0 40.0 35 37.906906420052444 40.0 38.0 40.0 38.0 40.0 36 37.72867736205487 40.0 38.0 40.0 32.0 40.0 37 37.63753630135055 40.0 38.0 40.0 32.0 40.0 38 37.51412580708828 40.0 38.0 40.0 32.0 40.0 39 37.33118109792201 40.0 38.0 40.0 32.0 40.0 40 37.21986635463952 40.0 38.0 40.0 32.0 40.0 41 37.06733019426509 40.0 38.0 40.0 32.0 40.0 42 36.89740462965574 40.0 38.0 40.0 27.0 40.0 43 36.70119406772493 40.0 38.0 40.0 27.0 40.0 44 36.47179772746497 40.0 38.0 40.0 27.0 40.0 45 36.19473031268503 40.0 38.0 40.0 27.0 40.0 46 35.91659852820932 40.0 38.0 40.0 13.0 40.0 47 35.62231792934277 40.0 38.0 40.0 13.0 40.0 48 35.32365297318634 40.0 38.0 40.0 13.0 40.0 49 34.97559703386246 40.0 38.0 40.0 13.0 40.0 50 34.740850649899905 40.0 38.0 40.0 13.0 40.0 51 34.21321228183945 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.33461966627132256 2201 2 -0.28380895238440473 2201 3 -0.4912245261049577 2201 4 -0.5346330399458452 2201 5 -0.07234658388577486 2201 6 -0.26122083152764475 2201 7 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1213 42 0.07406993398051753 1213 43 -0.4365247576176188 1213 44 -0.47430988793406215 1213 45 -0.26299283179426425 1213 46 -0.7113494314939217 1213 47 -0.25021387609073287 1213 48 -1.0287894436440155 1213 49 -1.1421399902550746 1213 50 -0.08119667423559207 1213 51 -0.16274482891244446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 8.0 16 17.0 17 9.0 18 11.0 19 17.0 20 13.0 21 25.0 22 55.0 23 103.0 24 221.0 25 342.0 26 566.0 27 783.0 28 1124.0 29 1676.0 30 2307.0 31 2806.0 32 3437.0 33 4074.0 34 4940.0 35 5759.0 36 7123.0 37 10091.0 38 16799.0 39 79562.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.61282123693872 15.778734820672128 11.625953120587406 21.98249082180175 2 16.478698508472664 21.818169002171032 40.202159754137654 21.500972735218653 3 21.998618434037272 29.136239321059016 27.75114895536696 21.113993289536754 4 12.316378605464234 17.179349818140807 36.295006625877576 34.20926495051738 5 17.001719908647477 35.26870048213833 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22.748611385231342 30.546705388107252 21.76318831589929 19 25.91916429356867 22.36445146192235 30.68345222319339 21.032932021315588 20 23.629712126765725 21.85482279301886 30.541066343361432 23.974398736853978 21 23.76786872303832 22.876194772605523 31.427806129641638 21.928130374714524 22 21.068176050976962 26.29486564975893 31.267798234978994 21.369160064285108 23 20.96808300673866 25.86841289085629 32.577466377195705 20.58603772520935 24 22.902275354554938 25.662587757633858 32.090393887275496 19.344743000535708 25 20.16945329461189 28.662559562410127 32.43367073617729 18.734316406800687 26 21.15699100572363 27.602419150195956 32.33287281134576 18.907717032734656 27 19.817717878591367 29.198268813263034 31.710463247525873 19.273550060619733 28 18.785772690106295 27.711675642146222 31.38339865226831 22.119153015479178 29 19.14667155383878 28.236811684100715 30.360616911495192 22.255899850565314 30 19.133278822567455 28.2389263258804 30.474102687004823 22.153692164547326 31 19.71762483435306 27.64330222460315 32.02413511151211 20.614937829531677 32 20.05385287732258 29.63177037809795 30.09910621140779 20.21527053317168 33 21.983111060986268 28.678066935461132 29.82420278004906 19.514619223503537 34 19.64713677503031 30.43815377675022 29.833366227761015 20.081343220458454 35 19.776129923590943 31.24453717540249 29.68745594496292 19.291876956043648 36 19.382101671976766 29.98209603293202 31.471508726421742 19.164293568669468 37 19.303155045535288 31.728085262356558 29.184171201398485 19.784588490709673 38 19.463867820791158 30.10474525615361 30.76451349141455 19.66687343164068 39 19.016973524684918 30.33312656835932 28.85710660614092 21.792793300814843 40 18.99441734570164 30.45577579158091 30.665125327769477 19.88468153494798 41 19.09310062875349 30.133645360475935 28.5286322496969 22.244621761073674 42 21.527053317168072 29.700143795641022 27.686299940790033 21.086502946400877 43 19.320777060365973 29.520399244368008 28.88248230749711 22.276341387768912 44 18.909831674514336 31.534243099218994 27.9315983872332 21.62432683903347 45 19.217864493754757 29.21307130572081 28.671723010122086 22.897341190402347 46 21.089322468773787 29.163729664194886 27.14636140637776 22.600586460653567 47 19.846617982913695 28.22764823638876 26.246228888826234 25.679504891871318 48 19.09310062875349 28.23258240054135 26.536639693235962 26.137677277469194 49 21.415833139489806 27.190255661993273 25.998999062502204 25.394912136014717 50 19.44977020892661 27.337384047142415 28.15575041587955 25.057095328051425 51 19.172752135788198 27.284518002650348 26.26455578425015 27.278174077311302 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12.0 1 11.0 2 10.0 3 23.5 4 37.0 5 63.5 6 90.0 7 87.5 8 85.0 9 159.0 10 233.0 11 463.0 12 693.0 13 1457.0 14 2221.0 15 2938.5 16 3656.0 17 3857.0 18 4058.0 19 3748.5 20 3439.0 21 3402.5 22 3366.0 23 3852.0 24 4338.0 25 4513.0 26 3949.0 27 3210.0 28 2878.5 29 2547.0 30 2592.5 31 2638.0 32 3147.5 33 3657.0 34 3699.5 35 3742.0 36 3521.5 37 3301.0 38 3123.5 39 2946.0 40 3114.0 41 3282.0 42 3548.0 43 3814.0 44 4349.0 45 4884.0 46 5627.0 47 6370.0 48 7438.0 49 8506.0 50 10156.0 51 11806.0 52 11106.5 53 10407.0 54 10383.0 55 10359.0 56 9636.0 57 8913.0 58 8000.0 59 7087.0 60 6143.5 61 5200.0 62 4511.5 63 3823.0 64 3322.0 65 2821.0 66 2308.0 67 1795.0 68 1530.5 69 1266.0 70 1108.5 71 951.0 72 795.5 73 640.0 74 541.5 75 348.5 76 254.0 77 196.0 78 138.0 79 110.0 80 82.0 81 60.0 82 38.0 83 25.5 84 13.0 85 10.5 86 8.0 87 4.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16071277525587166 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 7.048805932275072E-4 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 141868.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.23261489627664 #Duplication Level Percentage of deduplicated Percentage of total 1 87.03199682891834 58.51388726452637 2 7.710317740409085 10.367696467377078 3 2.269865837129634 4.578270470818538 4 0.9884827664373658 2.6583312466995834 5 0.5986390228219856 2.0124033441636957 6 0.32713222693212346 1.3196373020093302 7 0.2076159584426261 0.9770994646206119 8 0.1603879958786969 0.8626643488718426 9 0.07921452124690741 0.47932194610676254 >10 0.5510999463109545 6.305347094627691 >50 0.04047734921123687 1.8847279917684554 >100 0.025288057277414636 3.324377835475651 >500 0.007374693653969139 3.9332687400289785 >1k 0.0021070553297054683 2.782966482905431 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC 2575 1.8150675275608312 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1354 0.9544083232300448 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 982 0.6921927425494121 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 896 0.6315730115318465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 852 0.6005582654298361 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 848 0.5977387430569261 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 701 0.4941212958524826 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 648 0.4567626244114247 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 626 0.4412552513604196 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 490 0.34539149068147856 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 335 0.2361349987312149 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 302 0.21287393915470718 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 297 0.20934953618856966 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT 254 0.17903967067978685 RNA PCR Primer, Index 44 (95% over 21bp) ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 248 0.1748103871204218 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 230 0.1621225364423267 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 225 0.15859813347618915 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 216 0.15225420813714158 No Hit GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA 214 0.15084444695068655 No Hit ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA 205 0.14450052161163898 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 192 0.1353370738996814 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.10291256661121605 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.10220768601798855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 7.048805932275072E-4 10 0.0 0.0 7.048805932275072E-4 11 0.0 0.0 0.0014097611864550144 12 0.0 0.0 0.0014097611864550144 13 0.0 0.0 0.0021146417796825217 14 0.0 0.0 0.0021146417796825217 15 0.0 0.0 0.002819522372910029 16 0.0 0.0 0.005639044745820058 17 0.0 0.0 0.0063439253390475655 18 0.0 0.0 0.009163447711957595 19 0.0 0.0 0.012687850678095131 20 0.0 0.0 0.01762201483068768 21 0.0 0.0 0.06202949220402064 22 0.0 0.0 0.11278089491640116 23 0.0 0.0 0.22767643161248485 24 0.0 0.0 0.341867087715341 25 0.0 0.0 0.3806355203428539 26 0.0 0.0 0.41587955000422927 27 0.0 0.0 0.45041869907237714 28 0.0 0.0 0.5194969972086728 29 0.0 0.0 0.6661121605999943 30 0.0 0.0 0.8705275326359715 31 0.0 0.0 1.0439281585699383 32 0.0 0.0 1.2180336650971326 33 0.0 0.0 1.3956635745904644 34 0.0 0.0 1.5725886034905687 35 0.0 0.0 1.7473989906109906 36 0.0 0.0 1.95110948205374 37 0.0 0.0 2.213325062734373 38 0.0 0.0 2.5269969267206136 39 0.0 0.0 2.905517805283785 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 335 0.0 38.953392 45 GACCCTA 360 0.0 36.248295 26 GTATGCC 360 0.0 36.248295 42 ATGCCGT 360 0.0 36.248295 44 ATCTCGT 350 0.0 35.99831 37 CCTAGAA 365 0.0 35.751743 29 TATGCCG 365 0.0 35.751743 43 ACCCTAG 365 0.0 35.751743 27 TCGTATG 365 0.0 35.751743 40 CGTATGC 370 0.0 35.268612 41 TAGAATA 370 0.0 35.268612 31 CACGAGA 370 0.0 35.268612 21 CCCACGA 370 0.0 35.268612 19 CTCGTAT 360 0.0 34.998356 39 TATCTCG 360 0.0 34.998356 36 CCCTAGA 375 0.0 34.798363 28 GAGACCC 375 0.0 34.798363 24 AGACCCT 375 0.0 34.798363 25 ATATCTC 365 0.0 34.518925 35 CCACGAG 385 0.0 34.478897 20 >>END_MODULE