FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_61.3410000000b0ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_61.3410000000b0ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141868
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC25751.8150675275608312No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.9544083232300448No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9820.6921927425494121No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8960.6315730115318465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.6005582654298361No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8480.5977387430569261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.4941212958524826No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6480.4567626244114247No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6260.4412552513604196No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4900.34539149068147856No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3350.2361349987312149No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.21287393915470718No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2970.20934953618856966No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT2540.17903967067978685RNA PCR Primer, Index 44 (95% over 21bp)
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2480.1748103871204218No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.1621225364423267No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2250.15859813347618915No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.15225420813714158No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA2140.15084444695068655No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA2050.14450052161163898No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1920.1353370738996814No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.10291256661121605No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.10220768601798855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3350.038.95339245
GACCCTA3600.036.24829526
GTATGCC3600.036.24829542
ATGCCGT3600.036.24829544
ATCTCGT3500.035.9983137
CCTAGAA3650.035.75174329
TATGCCG3650.035.75174343
ACCCTAG3650.035.75174327
TCGTATG3650.035.75174340
CGTATGC3700.035.26861241
TAGAATA3700.035.26861231
CACGAGA3700.035.26861221
CCCACGA3700.035.26861219
CTCGTAT3600.034.99835639
TATCTCG3600.034.99835636
CCCTAGA3750.034.79836328
GAGACCC3750.034.79836324
AGACCCT3750.034.79836325
ATATCTC3650.034.51892535
CCACGAG3850.034.47889720
Produced by FastQC (version 0.11.2)