FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_60.3410000000b0ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_60.3410000000b0ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992067
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC19760.19918009569918163No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG3050.036.8837618
TATGCCG3150.035.7128543
CGTATGC3100.035.5630841
TCGACGG3400.034.41037829
ATGCCGT3400.033.08690344
ACGGCAT3300.032.72595232
GTATGCC3400.032.42516342
CTCGACG3600.031.87371428
CCCACGA3600.031.2487419
GGCATCT3550.031.05508634
TTCCCGC453.4913282E-529.9987928
TGCCGTC4000.029.24882145
GACGGCA3800.029.01198631
CTCGTAT3850.028.6352139
TCGTATG3850.028.6352140
CCGAGCC3950.028.47986414
CTCCGAG4000.028.12386712
GACTCGA3950.027.91026726
CACGAGA4000.027.5613921
ACGAGAC4150.027.10734222
Produced by FastQC (version 0.11.2)