FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_58.3410000000b084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_58.3410000000b084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128508
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC43090.38183158648410115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4800.036.56282445
ATGCCGT5700.031.18448644
TATGCCG5650.031.06222243
GCCCACG5750.030.52065718
CCCACGA5850.029.99893619
CGAGCCC6000.029.24896415
AGCCCAC6300.028.21328717
CCGAGCC6350.027.63681614
CGAGACT6500.026.99904423
TCCGAGC6600.026.93086613
CTGGACC6750.026.66572230
GTATGCC6700.026.5289142
CCACGAG6900.025.43388220
CATCTCG6950.025.25090436
GAGCCCA7150.024.8592616
TCGTATG7100.024.71743440
GTATCAA15500.024.5673081
CGTATGC7250.024.2060441
CTCGTAT7250.023.89570639
ACTCTGG7450.023.85821527
Produced by FastQC (version 0.11.2)