FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_57.3410000000b077.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_57.3410000000b077.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC50.48543689320388345No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC40.3883495145631068No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC20.1941747572815534No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTT20.1941747572815534No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC20.1941747572815534No Hit
CCGATTCACTCTGCGTAGATTGACTGAACGCAGAAAGCCTTCCACTGGCAG20.1941747572815534No Hit
CCACTTGACACCCCATTAGGATTCGGTTTCCCTACGGCTACCCCACACGGG20.1941747572815534No Hit
CCAATGCGCTGCGCGAGGCCGCCTTCGCGCTCGGCACGCCCAAGTGGAAGA20.1941747572815534No Hit
AGCTATATTGTGGTCGAAAGATTTCTTACCAAATGTTCGAAATGCCCAGAT20.1941747572815534No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)