FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_54.3410000000b041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_54.3410000000b041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765774
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC14470.18895914460402155RNA PCR Primer, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2150.037.673645
GCCCACG2250.035.99921818
TATGCCG2350.034.46733543
CGAGCCC2400.033.74926815
CCGAGCC2450.033.06050514
ATGCCGT2500.032.39929244
CCACGAG2600.032.0185320
CGTATGC2550.031.76401541
CCCACGA2600.031.15316619
AGCCCAC2700.029.9993517
CGGGGGT453.489856E-529.99934817
CTCGTAT2800.028.92794239
CGAGACC2800.028.92794223
GTATGCC2900.028.70627242
AGCACCC406.165034E-428.124395
CCTGTGG3000.027.74939528
TCCGAGC3050.026.55679913
ACCTGTG3150.026.42799827
GTGGACA3100.026.12846231
GAGCCCA3200.026.0150616
Produced by FastQC (version 0.11.2)