FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_52.3410000000b027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_52.3410000000b027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202880
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC163481.3590715615855282No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT20680.17192072359670127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC18150.043.015145
ATGCCGT20700.037.8248344
GCCCACG21600.036.87377518
CGAGCCC21750.036.51602615
CGAGACC22000.036.20334223
CGTATGC21800.036.12265441
TATGCCG21750.036.10224543
CCGAGCC22050.036.0192114
CCCACGA22250.035.89768219
GTATGCC22100.035.73410442
AGCCCAC22300.035.716317
TCCGAGC22350.035.5357313
GAGCCCA22500.035.39882716
CCACGAG22650.035.36306420
TCGTATG22400.035.1550840
ACGAGAC22750.035.00982722
CATCTCG22750.034.61423536
CTCGTAT22700.034.59136239
CTCCGAG23250.034.35369512
GACCATC23700.033.79634526
Produced by FastQC (version 0.11.2)