FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_51.3410000000b01a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_51.3410000000b01a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC30.23734177215189875No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGAT20.15822784810126583No Hit
CCCTTGGGACCTTCTTCAACCCCAGGATGTGATGAGTCGACATCGAGGTGC20.15822784810126583No Hit
ATCCAAAGAGAATACAGATCCCCAGCCACAGGTTTGTGAGCAGATTGTGAT20.15822784810126583No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGG20.15822784810126583No Hit
GGATCTCACTTCTTCTTGGCATCACCAGCCTTTGTCTTCTTAACACCACGG20.15822784810126583No Hit
TCCAGGGAATCCTCATCTTCAGGCGAGTTTCCCGCTTAGATGCTTTCAGCG20.15822784810126583No Hit
TTTTATCTCTTTCTAGGAAATGTAACCAACGGTTGTGATCAATGCCAAGTC20.15822784810126583No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.15822784810126583No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers