FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_50.3410000000b00d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_50.3410000000b00d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA21.4285714285714286No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.4285714285714286No Hit
CAAGAAGCTGCATTGCCTTCTCACCTCTCACAGTCACATAACACGCAATCT10.7142857142857143No Hit
GTCCTCCAGTACGTGTTACCGCACCTTCAACCTGGCCATGGATAGATCATC10.7142857142857143No Hit
GTGTATACAAGGATTATGGGAACTCACATCAATTTCAACAACTTAGGAGGT10.7142857142857143No Hit
GTACACACTTTTTCCAAGTTCTTGACATTCTTGGAGGAGAGAGTAATTCTG10.7142857142857143No Hit
CCACTTCACCTAACAACAATGCGCAGACGCGGCAATGGCTGTCTCTTATAC10.7142857142857143No Hit
GTCGAGCACGGCCTGCGTAAACTGCTGGGTCGTCGAGCTGCCGCCCATGTC10.7142857142857143No Hit
TATCAACGCAGAGTACGGGGATTTAGGGTCTGTAATTTGTATAAGATGATC10.7142857142857143No Hit
GTCTTGACCTGGATGATGCTGTCCTGACGCAGCGCCACGCGCACGATGGAA10.7142857142857143No Hit
CTCTCCTACCACGCACTTGCGTGCGTCCGCAGCTTCGGTGACTGGCTTAGC10.7142857142857143No Hit
CTTAAGAAAAACTCTAAAAGTAAATACACATTTCTCTTGTTGTGACACAAA10.7142857142857143No Hit
GCACTAGGCCGCTCGCCCCACATCCTTGAATTCCGCGCCCGGATTTGCCTA10.7142857142857143No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAAC10.7142857142857143No Hit
CTGATCAGATTAGCTTTAAGCAGGAACCCTTGGAATTTCGGCGAGAGGGCA10.7142857142857143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTATAC10.7142857142857143No Hit
CGCATGGGTGTGCAGGAGTGCGCGAACCATGGCCTTGTGACACCGTTCCAG10.7142857142857143No Hit
GCAGGAGAGAAAGTGGTGATACTGAATTTCTGGTCACTGTCTGTCTCTTAT10.7142857142857143No Hit
GCTTACGTTGGTGATGAAGCTCAGTCCAAGAGAGGTATCCTCACTCTGAAG10.7142857142857143No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC10.7142857142857143No Hit
GTGAAGGAAAGGTGAAAAGCACTTCGAATAGAAGGGTGAAAGAGAACCTGA10.7142857142857143No Hit
TACTATGTGTGCTCCCTGACGAAAAAAAAAAAAAAAAAAAAAAAAAGTACT10.7142857142857143No Hit
GTTGGTATTGCTGCCACCACAACATAGGCTCAGAGTGCTACTTTTGGACTT10.7142857142857143No Hit
GTTGATGACCACAGGTCTGGAAAGATCGTGGTCGAGCTTAATGGAAGACTT10.7142857142857143No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCA10.7142857142857143No Hit
GCATGGAGAAGAGGATTATACCAAGGTGTAACATACTCGAAGCTCTGTCTC10.7142857142857143No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGAT10.7142857142857143No Hit
GTCCTCCAAGGCCTGTTAGGGCTCTCTTCAACCTGCTCATGGCTAGATCTG10.7142857142857143No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACCGGGACTTCAATCAAGA10.7142857142857143No Hit
GTATCAACGCAGAGTACGGGCAGAATTCAATGATCATCACACAAAAAATAA10.7142857142857143No Hit
TCGCAGTCGGGTCCGGCGTGAGCGTGGGCTGCAGCGCGTCTTCCACCGCGC10.7142857142857143No Hit
CGGGCCAGCCGTGCAACAGCACCTCGGGCGGCCGCACGGCCCACCGCCGCT10.7142857142857143No Hit
GTGCATCCGCATGTCCTTCTCACGGCGCCCCAGCTGCGGCGCGTCGACCGT10.7142857142857143No Hit
CTTTCGCACCTCAGCGTCAATACTTGTCCAGCGAGTCGCCTTCGCCACTGG10.7142857142857143No Hit
GGTATCAACGCAGAGTACGGGGTACTTTTTTTTTTTTTTTTTTTTTTTTCC10.7142857142857143No Hit
CGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.7142857142857143No Hit
GTACAAAGTACACAAACCGTAGTTAAAACTTTGGAAACGTATCTAGGGATC10.7142857142857143No Hit
TAACTTGAGAATCTTCCAACACAGAAAACAAGAAGAGAGAAAAGTAAACTG10.7142857142857143No Hit
GTCCGTGCTTGTCGAAGAAACGAAGAATTTCAAGGCGTCTGTGGGCATCTT10.7142857142857143No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTGACTGTTTTAGCCTTCC10.7142857142857143No Hit
CGCATCTACTGCTCCGATACCATTCTTGGCGTATCCCATACGCCACGCACG10.7142857142857143No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAC10.7142857142857143No Hit
CCTGTATCTCACGTATGAACATCGCCTCCCAGTCAGCAGCCACGCCATCCC10.7142857142857143No Hit
GAACCAGTACCGTGAGGGAAAGGTGAAAAGTACCCCGGAAGGGGAGTGAAA10.7142857142857143No Hit
ACAGTCGCTACCCCCTGGCCTGTGCCCCCCACAAAAAGTTGCCTTAATGTG10.7142857142857143No Hit
GTACCAAGCGTACCTTCGAACTCCTCCGTTACACTTTGGGAGGAGACCGCC10.7142857142857143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT10.7142857142857143No Hit
GTTACAGGGAAGGAGTAGATAAGTCCTGATGGAACGCTGTAAGAGCCATCG10.7142857142857143No Hit
GCACATTGCGAAGATACCTCATCCTTCCAGTTCCAGTAGTCTTTCTACGGA10.7142857142857143No Hit
ACCATAATGGGAGTTTCAAAGAAATACCACAAGCTAATCCAAAAACATAGT10.7142857142857143No Hit
CCACAGGAATGTCCAGCGCTGCCAGCAGCTCATCGTCCCAGCGTTGCGTGT10.7142857142857143No Hit
CAACGCACTCAAGCAGCCTCAGAAGAAGATTGGTAGTCGTATGCCGTCTTC10.7142857142857143No Hit
GCTTTCTCGAGTCTGACAGTAAGGTCAACAACTGAGACATCAACGGTTGGG10.7142857142857143No Hit
GAGTACGGGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.7142857142857143No Hit
GTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACC10.7142857142857143No Hit
ACCGTGGAGACTGATAGATCTCGTAGGAGATTGTGAGATTAGGGTCTGTCT10.7142857142857143No Hit
GTATCGGGTGGCGGCATCGGCGCGGGGCTGAACAGATTCCATGGCCTGTCT10.7142857142857143No Hit
CTCTGCATAAACATGACTTTCAAGCGCAGGAATGGTGGCAGGAACAAGCAT10.7142857142857143No Hit
ATTATATCATCATTGATGGTGCACACCATAAAATAGGAACATGAGCTACCT10.7142857142857143No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG10.7142857142857143No Hit
TTCCCTAGCTATGCCTACGCGCTGCTGCTGCCCCCCAGACCTGTCTCTTAT10.7142857142857143No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTACCAAAAAAAAAAAAAAAAA10.7142857142857143No Hit
GCGCAGCACCTCGACCCGCTCGACCTCGACCAGATCGATCCAGTTTGCGGT10.7142857142857143No Hit
AAACAGAGAGACACAGGAAATTATGGGGCAAAATCATACAAATGTTATTAG10.7142857142857143No Hit
AGTTTCCACCATTCACAAACGATTCACACGGAGAGCTTTTGATTAACCGGA10.7142857142857143No Hit
TCCTTAAGGTCAAGCTCAAGTCCAAGAAGATGCGTGTATTCTCCAGACCCA10.7142857142857143No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCCCTCCCGT10.7142857142857143No Hit
GATTCAAGAAGAACAGGAAGAAGAGAGGTCACGTCAGCGCCGGACATGGAC10.7142857142857143No Hit
CTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATC10.7142857142857143No Hit
CTTTCAACTTCTTCCTCTGTTTTCCCTTTCATCTCAGAGGCAATGCTTTTT10.7142857142857143No Hit
GCAAGCGACCATGGAAATTCTCCACCATCGTCTACTCTTCATCGATCTTAT10.7142857142857143No Hit
CTATATAAAAGGTAGTTTTTTTCTAAGTTCTACGTACAATCACAAAATCCA10.7142857142857143No Hit
ATTTTTACCCTTGAGGAGCAAAATTCCAATTCAAGAACGGTTTCTGCTCGA10.7142857142857143No Hit
CTCCTGGGTTCAAGTGATTCTCCTGCTTCAGCCTCCCGAGTAGCTGGGATT10.7142857142857143No Hit
GTAGTAGCATAGACTGGTGGTTAGATGGGCTAAGCGGTGAACAATGGAGAG10.7142857142857143No Hit
CCTTTTGCCATGGCTCACTGAACTTTGAACAATATTATACCTGTCTCTTAT10.7142857142857143No Hit
GACCAGAACCAGGTTTGACCGAACGAGGATGCGAGATGACCAATCCGCAAC10.7142857142857143No Hit
CTCTTCTTATCCTTCTTCCATCTCAGAATTTTGCATTCTGTTTTTGTTCTG10.7142857142857143No Hit
GTCCACTACTGAGTATTTAATGTCAAAAACAGAAAAGACTGGAAAGAAAAA10.7142857142857143No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTACAC10.7142857142857143No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCCCG10.7142857142857143No Hit
TCTTTGACTCATGTATTTTGTAAAATTGCCTTGATTATGCAATGTGAGATG10.7142857142857143No Hit
CTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGG10.7142857142857143No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10.7142857142857143No Hit
TATCAACGCAGAGTACGGGTAGAGTATGTTTAAACGCTAGAAGCTTTTCTT10.7142857142857143No Hit
CCCTAGCCTTCTCAGCCTTTGGCTTGTGGATACTCTCCATCTGTCTCTTAT10.7142857142857143No Hit
GCTGTACCGAAGAAGAAGTCTAAGAAGTGAAGAGGATATATAGCTGTCTCT10.7142857142857143No Hit
CCATATATCAAGAAAGATGAACAACAAGAGTCATTGTCTGTCTCTTATACA10.7142857142857143No Hit
CAGCCCAGATCGCCGGTTAAGGCCCCTAAGGGTGTACTAAGTGGAAAAGGA10.7142857142857143No Hit
TTAGGAGACAGATAAAAACTTGACTTTCTCATTTCAACGGACACACTTTCT10.7142857142857143No Hit
ACCCCGGATACCGCTATCGCCCCGATGGAGCCGTCCACTACCGCCCCTTGC10.7142857142857143No Hit
CCCTTAGGGGCCTTAACCGGCGATCTGGGCTGTTTCCCTCTCGACTATGAA10.7142857142857143No Hit
CTGGAGGTTCGTTCGTCCTGGTCCTCTCGTACTAGGGACGACTCCTGTCAA10.7142857142857143No Hit
ACACGTCATTCCTTGGAAACCCTTTTCTCTGTGGATTTTCTTCAAACCCTT10.7142857142857143No Hit
CCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGCGAATCAACGGTTC10.7142857142857143No Hit
GGACCTGCATTATCAAAGCAGTGACGTCCCGTACTCTGCGTTGATACCCTG10.7142857142857143No Hit
CTTGTATATTGCCTGGCCATTGTCAAAAGCTCTGAACTCAACATTATTGGG10.7142857142857143No Hit
CAACATTACTGGTTCATACACCTTTTACAGGATCATTCAACACAAGTACAC10.7142857142857143No Hit
TCTTCACGGAGAATTCAATTTCGCTGAGCATATCCTGGAGACAGTGGGGAA10.7142857142857143No Hit
AGTTAGATGTGTGCATTACATGTTGATGATTAGTGTCTTCATGATCTGTCT10.7142857142857143No Hit
AGATAAATCTCTCTTAGTATTGAAATCATGTTCAAGTCCTGTTGAACCTGA10.7142857142857143No Hit
GAAATATGCCATAACACAGAAGCCTAATAAGATGTCTACATCTCTGTCTCT10.7142857142857143No Hit
TGTAAGACTTGCTGCTTCTGTTTTAAGTTACTACTTGTGTGTTGCACTTCC10.7142857142857143No Hit
CTGCTTTTGTGCGGAACGAAAAGACTCTGGTTAATACCTGGGGTCCATGAC10.7142857142857143No Hit
AGTGAATAAGGAAGAAGAAGATGGAGATAAGGATTGTGGTAAAGGAAACAA10.7142857142857143No Hit
CAAAGTCCGTTATGACTATAGCGTTTCAGCTAGCTTTTGAGATGCATATGC10.7142857142857143No Hit
CCATTACGTATTACAGACTTCTTGACGAAAGATTGAACCTTTTAGCCATAT10.7142857142857143No Hit
GGGGTATATACCCTACCCAAAGGTCAGTTTCAGATAGAAAATGGTTTAGTT10.7142857142857143No Hit
ATGTTATGTAACTATTGTGTGTGAGGATCTTTGTGCTGTCTCTTATACACA10.7142857142857143No Hit
CTCTCGTACTAGGAGCAGCCCCCGTCAAGATTCCAACGCCCACGGCAGATA10.7142857142857143No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGTTC10.7142857142857143No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.7142857142857143No Hit
CCTCTCTGGGGAAATGTTTTTGTTGTTGTTGTTGTCTCGGTTCTACAGTGA10.7142857142857143No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGGATTATCTCGCATGCAGTC10.7142857142857143No Hit
GTACCTCTTCGCCTTGATCAATGGTAGCTTCCTGATTGCGTGCGCTGTTTC10.7142857142857143No Hit
CTCCAATGCAGCTCTCATCACGGAGACATTCCCGATGCACTCAAAGCTGTA10.7142857142857143No Hit
AGAAGGCAAGTTAGTGATAAAAAAGCTAGAATAAGAACAATAGCTGTCTCT10.7142857142857143No Hit
ATTGTAACCTTTTGAAATCTATATGTTGTTGTTGTTTAGTTTGTATTCGAT10.7142857142857143No Hit
GTGTACCGTAAACTTACTGGCAAAGATGTTGTTTTTGAGTACCCAGTCGAA10.7142857142857143No Hit
ATATTATTGTGAACTCGATTGGTGTGTCTAAGGAGTCACAGGTTCGAACAG10.7142857142857143No Hit
GTACTGAATCCTCGGCTGCTTCATCATAATCCTCTTCTTTATACCACGGAT10.7142857142857143No Hit
NACATATTTCTCTAACGTTGGTCACAAAACTCTTACATTGTTTAAGCGCTG10.7142857142857143No Hit
CTATAACTCAATCATGAAGTGTGATGTGGATATCAGGAAGGATCTGTACGG10.7142857142857143No Hit
GTATCAACGCAGAGTACGGGATGCAATGCGGCGGCTGCATACGCTCTGTCT10.7142857142857143No Hit
GTGCGCGCTGGGGCATGGCACCGTCTACCCAGGCGGCAATCTGCACAGGCT10.7142857142857143No Hit
GTACAACACTGATGAGTCCATCCGTGCTTTTGCTGATGCATCGATGAACAC10.7142857142857143No Hit
GACCGAGGGTGATGCCGGGGCCGGGGTAGGTGCCGTCCATGATCGAATTCA10.7142857142857143No Hit
AGAGAGAAGGAGCAGCGTTACGAGTTTTTGATGGGGTTGAACAAAGAGTTT10.7142857142857143No Hit
ACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCGTCAAGATTCCAACGCCCA10.7142857142857143No Hit
AGATGAAGGAATCAAGTTGGTTGCTGAAGCCATATGCTCTGGTATATTCCT10.7142857142857143No Hit
CTTTTAAGAAACAAATGTTTTGTTGTACTGAAACTCAAGTTCTTTTGCTGT10.7142857142857143No Hit
AAGGTACGCCCTTGTAGCTTGACTGGCCTGCGCCAGGAGGGTGAAGGGGTT10.7142857142857143No Hit
CCATAGCTCTGATCTTGAGATGAGGCCATTGTAGTCCCTGTCTCTTATACA10.7142857142857143No Hit
AAGCTACCGTCCGAAAATGGCTGGGGAAAACGGCTCATGACGTCGGCGCGC10.7142857142857143No Hit
TTTTATCAGGCCGTGAAACATAGTCTTCCAAGTCAACCTCATCGCTAAGGT10.7142857142857143No Hit
TATCAACGCAGAGTACGGGCAGGAGACAAGAAGGATGATGCCGTTAAGGAG10.7142857142857143No Hit
CCGCTGAAAGCATCTAAGCGGGAAACTCGCCTGAAGATGAGGATTCCCTGG10.7142857142857143No Hit
TGTCTGTTCTGGATGGTCAGTTTCACTGTAATTCTGAGACCCTTCCAGTCA10.7142857142857143No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers