FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_5.3410000000aca6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_5.3410000000aca6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862427
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC800.036.5613245
AGGACCG352.8203306E-432.1418235
GGGGGGT300.005147365429.99903319
CGAGCCC952.3646862E-1128.42013715
GTATGCC1058.54925E-1125.71345742
CCGAGCC1058.54925E-1125.71345714
GAGCCCA1159.094947E-1225.43396216
GCCCACG1101.5097612E-1024.54466218
TATGCCG1101.5097612E-1024.54466243
CGGGGTG551.368115E-424.5446626
GGACCGA551.368115E-424.5446626
ATGCCGT1101.5097612E-1024.54466244
AGCCCAC1152.6557245E-1023.47750517
CCCACGA1152.6557245E-1023.47750519
CCACGAG1152.6557245E-1023.47750520
ACGAGAC1204.5110937E-1022.49927522
GCCTCGG500.00226457922.49927334
CGAGACA1301.2259989E-920.76856223
TCCGAGC1351.9626896E-919.99935513
AGCGGAG908.249624E-619.99935520
Produced by FastQC (version 0.11.2)