FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_49.3410000000aff3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_49.3410000000aff3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259866
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGCA401.5564414E-533.74841731
CCCGCAT401.5564414E-533.74841732
GCATAGG300.00508819130.0622181
GCCGGAA300.005141198629.99858711
GTCCCGG602.4077963E-729.99858726
CGTCCCG602.4077963E-729.99858725
CGCCACG654.850972E-727.69100420
ACGTCCC709.26495E-725.71307624
GTTATAT450.001209325.0518471
CATCCCG551.3646623E-424.544329
TTAACCG651.5184156E-524.22962843
CCCGGCT651.5184156E-524.22962815
CTAACGT952.100569E-823.6830983
GTAGTAC602.4261088E-422.5466631
CGGTTCC802.9612893E-622.49894330
CCACGTC802.9612893E-622.49894322
GCCACGT802.9612893E-622.49894321
TCGCCAG500.002260549522.49894138
TAAGTCA500.002260549522.49894121
CGGAATT955.500315E-721.31478736
Produced by FastQC (version 0.11.2)