FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_46.3410000000afcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_46.3410000000afcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860499
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC250.00210721535.99860838
TAGGCGG453.4907585E-529.9988374
GGGGCAA755.6377758E-826.99895518
GCCACGT551.368172E-424.54450421
TCGCGCG952.114757E-823.6832929
TCGGGCG1003.643254E-822.4991337
AGGCACG500.0022646622.4991325
CGTCGGG1003.643254E-822.4991314
GTCGCGC903.2861863E-722.4991288
AGTCCGG802.9743132E-622.49912810
CCGTAGT602.467456E-422.49912838
GCGGGGG802.9743132E-622.49912825
CGGGGCA1056.105802E-821.42774217
TACCCGG955.5304554E-721.3149626
GGTCGCG955.5304554E-721.3149627
ATACTGG955.5304554E-721.3149626
GTCCGGA855.03147E-621.1756511
GGGGCCT754.6022797E-520.99918628
GGTCTAC550.00390098720.4858931
CAGCCCC1009.0528556E-720.24921628
Produced by FastQC (version 0.11.2)