FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_41.3410000000af7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_41.3410000000af7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733677
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA32490.442837924590794No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT27120.36964495275168774No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA22810.31089975561452793No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG16200.22080561336937102No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT14190.19340936134020828No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC9110.12416908257993639No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAA8010.10917610883263344No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG401.5601723E-533.7488259
CCGTTAC3600.031.2979721
CCCACGA1300.031.15275819
TGGAGGG300.00514693629.9989575
GCCCACG1350.029.99895718
TGCCGTC1550.027.57968545
CGTATGC1500.026.9990641
ACACCGA755.635229E-826.999066
TATGCCG1500.026.9990643
ATGCCGT1500.026.9990644
GCGCCAT608.188485E-626.24908621
GTTCGTA1350.025.0383761
GTTACTA4650.024.6765583
CCACGAC651.5231863E-524.2299239
CGAGACA1700.023.822723
GACAGGA1750.023.14205226
CGCGCGC602.4669108E-422.49921610
GTATGCC1800.022.49921642
TAAGGTG1104.0490704E-922.4992165
GATCTCG1700.022.49921636
Produced by FastQC (version 0.11.2)