FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_40.3410000000af6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_40.3410000000af6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC40.25046963055729493No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC40.25046963055729493No Hit
ACGTAATGCAAGCCTTGTGGTACTGTGTCTTTTTACACCTCCTATCACATT20.12523481527864747No Hit
GGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAA20.12523481527864747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12523481527864747No Hit
GCCCTGTAGATTCGTTCCTATGGTTCGATCCTTCCCAGTAAAACGCGGCGT20.12523481527864747No Hit
CTCTACTTGTTCACCTGTGTCGGTTTGGGGTACGGTCAGTTCACCGGGAGG20.12523481527864747No Hit
CTGTAGTAGTGTCGAAATGACAAAACTTAAAATCTCAAAGCAACTAAAGCA20.12523481527864747No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTA20.12523481527864747No Hit
GTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTAT20.12523481527864747No Hit
CGCTCATACATCACTTGTGATCTTTCCTTTCTCTTTGTCTCAAGTTCTTTA20.12523481527864747No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)