FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_30.3410000000aecd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_30.3410000000aecd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938785
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC19320.2057979196514644RNA PCR Primer, Index 20 (95% over 21bp)
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA13760.14657243138737835No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA11620.12377700964544597No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT11530.12281832368433666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2350.040.21143345
ATGCCGT2700.035.8321444
GCCCACG2650.035.65919518
CCCACGA2650.035.65919519
TATGCCG2700.034.99883743
CCACGAG2800.033.7488820
AGCCCAC2850.033.15679617
CGTATGC2900.032.5851341
GTATGCC2950.032.03283742
GGACATC2950.032.03283733
TGTGGAC2950.031.27015130
TCCGAGC3050.030.9825813
CGAGACC3000.030.74898123
CCGAGCC3100.030.48286214
CCTGTGG3050.030.244928
CTGTGGA3050.030.244929
GTCTACG300.00511018830.0438121
CACGGGG300.00514761829.9990068
TAGCGTA300.00514761829.9990067
ATCACCG300.00514761829.99900639
Produced by FastQC (version 0.11.2)