FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_3.3410000000ac62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_3.3410000000ac62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTAAGCTGAGTCCTCTCAACTAGTGTAATAGATTACAGGTCTTGTTCC10.641025641025641No Hit
CGTACAAGCGTGCTGACGGCAAGGGCACGTACAACTCGATCGTGATGCCCA10.641025641025641No Hit
GTAAACAGGAAGAAAATAATCAAGGAAGTGACCAAAGTAAAACGAAGAAGA10.641025641025641No Hit
AGATATTACTCCTGTGATGCTTGATTACAAGATTCATGATGAGAATAGTTC10.641025641025641No Hit
CTTGTATCTTTTCTTATCCTTTTTCCTGCCTTTATCATCTTAGCTATTTAC10.641025641025641No Hit
ATCACATTCCTCTGTTTTTCATTCTTGATCCTGTCTCTTATAACATCTCCG10.641025641025641No Hit
GCTGGACGATCATCACGTTGGGCATCGAATTACCCAGCAATCGCTTGACGG10.641025641025641No Hit
CTCACGTACCACTTGAATCGGCGAACAACCGAACCCTTGGGACCTTCTTCA10.641025641025641No Hit
CATATGGAAGTCGCCGTGGGAATGATGAAATAATGGCCAGAGGTACTTTTG10.641025641025641No Hit
GAAAATTGAGATAGAAAAACCTCAGAACACTAACAAAGTTGTTTTAGGTCT10.641025641025641No Hit
GAAAATAGTCTCATAAAAGATGAGTATTTTGTGACCTTTTGAGCATCCTAG10.641025641025641No Hit
GACAAGGATAAGGAGAGAAGGGAAAAGGAAAGAGACTGTCTCTTATACACA10.641025641025641No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTTAACCCCCC10.641025641025641No Hit
TAATAGATCTTGAACTGAATGCAACAAAGAAACTGGGGAGGAATGGCTTCC10.641025641025641No Hit
GTCTCAAGAAATGTGTAAAACAAGAATAGGAGAAATGCTGTCTCTTATAAA10.641025641025641No Hit
GTCCAAAGAGCGCAGACTAGCCACGGAGCTTGGATACGGTTTCCCGATCGG10.641025641025641No Hit
GGAATAAACTTTTGTGGAGAGGAGTGTTGAGAACGATTTGTTTCCTCTCTT10.641025641025641No Hit
ATATTTTGGAATGTTGTCAGTGGATCTTTATGGTGCTAGACATGAGAACTT10.641025641025641No Hit
AGAAAACTCATTGTGATGTTCAGTCCCATTATCATCATCACGTTGTAGCTA10.641025641025641No Hit
TTACAATGTCTTGGATAAGCTATTTGATCCTGAGAACTCTCCCAAGAGGGC10.641025641025641No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCTGTC10.641025641025641No Hit
ATTTTGTAGTAAGCAACTTTGTTGTTCGTTTTCAGAGGATTGTTTTATGGT10.641025641025641No Hit
AACATGAAGAGCAACAAATCACAGAGTTTGAAAAACACAGAAAGACATAAA10.641025641025641No Hit
GACTAAACACGCGTACATAATCTCAACTTTTTTTTTAGGATCTGGAAGGCT10.641025641025641No Hit
ACATAAATCAGAAAACCAAAATCTTTGCCTAGATATAAGAGCTGTCTCTTA10.641025641025641No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC10.641025641025641No Hit
GTATTGCATAGAGTATTATCAGCAGAAATATGACAACTAATGGGAACCTGT10.641025641025641No Hit
GGGTTTCAAGGAAAACCCGGCCTCTGGGCGCGGAAATTCGCGTTTGTTGCT10.641025641025641No Hit
CAGTATGCTACTGTCATGGAAAGTTTTTGTTTAAGTAGGAATCTAAAAGCT10.641025641025641No Hit
GTTTTCTCCCTGGGAATTTGGATTCCTCCACATAACTGCGTTTGTGTTATT10.641025641025641No Hit
TTTCTGCAGAGCTGGATCATAAGAAGAAGGACTACAAGTTTGGCTGTCTCT10.641025641025641No Hit
GTATCAGAACTGAAGTATCCTTCTCATTCCGGTGAAGAAGAATTACTGTCT10.641025641025641No Hit
CTTCATTATTGCACCTCATTTCTCTTTCTTTTCCCTGTCTCTTATACACAT10.641025641025641No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC10.641025641025641No Hit
GGAAGAACTATTGGGAAGAACAAAATTGATATCACTGTCCCTACCTGTCTC10.641025641025641No Hit
GACAAAATGTCACTGTTGTCTTTCTAGAAACAGAGAGAGAAAAGAATGGAG10.641025641025641No Hit
CTGGAGTTGCTTATCAGAAGGATTTGCTTCAACACTATAACATGGCCTGCA10.641025641025641No Hit
CCTTTAAGTATCTCTGTTTCGTTTGGAATTTCTACGTTTTGTAAGTACTGC10.641025641025641No Hit
ATGGTACAATGTTTTTGTCTTGGATTTTCTTTATAATCTGTCTCTTATAAC10.641025641025641No Hit
ATTCCAGTCACTGAGACAGGATTCCTGAATATCAGGATTGATACTGTCTCT10.641025641025641No Hit
TCTTCACTGTATGACTTGATGATAAAGAACCTGTCTCTTATACACATCTCC10.641025641025641No Hit
CATTATTATCATCCATCCTATTCTCACACAAGAGTTTTAAGAAAACAACTT10.641025641025641No Hit
CTTATACACATCTCCGAGCCACGAGACTCTGGACCATCTCGTATGCCGTCT10.641025641025641No Hit
GCCTAAGAGATGAAATGAGACGTAGAGAATCTGTTATGATCTCAGATATTT10.641025641025641No Hit
CTAGAAGCATTATCATAGAAAACACACAAGGCATATGGACACTGTCTCTTA10.641025641025641No Hit
CTACTGGCCTATGATGTTGATATTGACATGTAAAGACTTAGAAAGAGAACC10.641025641025641No Hit
GTCTTGCTCCTTATCCTTATGTAACCACTCAATGTTCCTGTTTCTTATACA10.641025641025641No Hit
TATAAGAACTAGAATCAGCTTTAAGGCATAACAAAAGTCTAAGACCTGTCT10.641025641025641No Hit
TCTTCAGGTGGGGCAAACAGGTAAAATAGTAGCACCAGAGCTATACCTGTC10.641025641025641No Hit
ATCGTAGAGGAAGAATGAATACTCATATTCCCCTCTTAGCTCATATTTCTT10.641025641025641No Hit
TTTTGGTTCTATCTTCCTTTTCTCTTCCAATATCCACTTGAAAAGCTCCTT10.641025641025641No Hit
ATGAAGAGATATTACCAAAACACAGGGACTTTGTTTTATTCTTTTGTGGGA10.641025641025641No Hit
ATAGAATCCTCTACTACACATACAGCGGTTTACATAGACAACACCTGTCTC10.641025641025641No Hit
GTACGTGAGTTGGGTTTAAAACGTCGTGAGACAGTTTGGTCCCTATCTGCC10.641025641025641No Hit
GAACTAGTAGTGCATCTGGATATCTCTCTCTCTTGTTTTGTTTTACAGTGT10.641025641025641No Hit
CCTCTACGAAACTTGCTACAATGGAGTTTTCATCACAAGACGACGCTTTTG10.641025641025641No Hit
GAATAATCACAAGGCATTCATAACGCAACACAAATAGCAAATGATTTGTTC10.641025641025641No Hit
GTTCTAATATTTGCCACGATATGATTTTTTGTTGTTGTTGTTTGTATGATC10.641025641025641No Hit
TTTTAACCTTGTTAAGAACCTACTTGAATCCTGTCTCTTATACAAATCTCC10.641025641025641No Hit
ATCTGGTAGTTTTACTCATGCGAGTTCTAACAAGGTATATCTTTGATTTCT10.641025641025641No Hit
CAGAAATCACTCTTTCAGCGTCTGATGTGAAGGATAAGTACAGGGACTGTC10.641025641025641No Hit
GCATTAGTGTATCCTACTAATGCAATTTGAAAAACTTGATACTGTTCACGT10.641025641025641No Hit
GTATTTTTGTTAGTGAGGAAATATGTGACTTATGCATGAACTTTCTTCTGT10.641025641025641No Hit
AAGTACCGTTGATGATCGATGTTGTTCACACAGGAGCATCATGTCTTTTGA10.641025641025641No Hit
GTTATCCCTAGCGTAACTTTTATTTCTTGAGCGATGATCATTCCTGTCTCT10.641025641025641No Hit
AATCTATATGTTGTTGTTGTTTAGTTTGTATTAGATCATCTTTTTTAGACA10.641025641025641No Hit
GTTCTCTGTTGAGGTTTTGTCGCTTTCATCGATTTTGCTTGAATCCTCTTC10.641025641025641No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAGAATACAA10.641025641025641Illumina PCR Primer Index 5 (96% over 28bp)
GTATTCGAGTCTCTCAAAATTTTATGAGCAATAGACACTGTCTCTTATACA10.641025641025641No Hit
GGAGAGGGCTATGAGAATCCAGTCTTTGAGAACCCCAGCAACCCGGATTGT10.641025641025641No Hit
CTTTGCCTATATTACACTATTCCAAGGGAGAGGGCTAAATTGATTTTAAAG10.641025641025641No Hit
TTATACGAGAGAGCTAAAACAAATGGATTCCAAATCCAGCAAGTACAAAAA10.641025641025641No Hit
GGCTGAGCTGAAGGAGAAGCTAGCAAGGATGTACGAGGTCAAGGATCCAAA10.641025641025641No Hit
AAATAGAGGTGAATGATGAAGAGAAAGAAGAGGAAGGAGAGGAAGAAGAAG10.641025641025641No Hit
ATTCTACATATCAAAATCAAAGCATCTCAATATCACATCTGTCTCTTATAC10.641025641025641No Hit
TTTTAAAGAAGCCCATTTTGTGGTGTTTTTTGGTTAGTACTATTTCTGTCT10.641025641025641No Hit
CCCAAAAGCTCTCCAAACTCAAAGGTTTGGGATAATACAAAAGTTACATAG10.641025641025641No Hit
GCCTACGGAACTATGATACCCCTTTTCACCATGAGAATGGTATCTGTCTCT10.641025641025641No Hit
CTATTAGAGATGTTCAAGAAGCTAGCGTCTACGAAGGTTACACTCTCCCAA10.641025641025641No Hit
GTTCTTTCACTTGTCTTCTGTCAAATAGTCAACAAATTTTGAATTATATCT10.641025641025641No Hit
CTCTCAGGCTATTCTGTATCGCCTTCACCAAGAGCTGTCTCTTATACAATC10.641025641025641No Hit
AAACAGGATCGATAGTTTGTCCATACGTAAGAACAGAGAATGTATAAGCCA10.641025641025641No Hit
ACTCAAGGAAACACGGTAGGCAACTCAGGAAAAAGGCTCCAAAGGCAAAAG10.641025641025641No Hit
ACCAGAAAGTTTTTAGTCTTCTCTGTTATAGTGAGATGATAAACAAGCCCA10.641025641025641No Hit
TTTCTATTTAGCTCCATGTGTTTCATTTTGTCTGAACCATTTTTGTTTGTG10.641025641025641No Hit
CTACAACATCCAGAAAGAATCTACACTTCATCTTGTGTTGAGGCTTAGAGG10.641025641025641No Hit
CTATAATGCTATTGCTGTCTTGGATTTTTCCACATCTGTCTCTTATACACA10.641025641025641No Hit
GTTTTAAACTTTGCGTTGAACTTGTAATCGATCTATCGACATTGAACCTGT10.641025641025641No Hit
ACATTAAAGATTGTGAACAAGGAACTGAATCAAAAGAGATTCCTTAATCCT10.641025641025641No Hit
ATTGTGGGAAGAGATATCAGCGGGAATGAGAAGGTTAGGATTCAACAGGAA10.641025641025641No Hit
TATCAACGCAGAGTACGGGAGTCTCTTATTTGTAATCGGAGTATTAAGTCT10.641025641025641No Hit
GTCGCGGTTCGCATGAGCAGCTGCTCTCAATGCTTTGTATCTGTCTCTTAT10.641025641025641No Hit
TACCATTACATAGTGTTACTGCTTCAGCTTTGCTCACTTCATTGCTATCTC10.641025641025641No Hit
GTTCTGTAGCTGCATGGTTCCAGTTTCAACAAACCAAAACTTCAAGATCCC10.641025641025641No Hit
GTTATTGTTGTTGTTGTTGTGTTTGATGTTTTGTGTTGTTTCGTTGCTGTC10.641025641025641No Hit
GTTCAACTACGAGCTTTTTAACTGCAACAACTTAAATATCTGTCTCTTATA10.641025641025641No Hit
ATTTTGAACAATGGTTGGTTCTATACCAGTTTAGCTACTGTCTCTTATACA10.641025641025641No Hit
CCTTTAAGGATACTATTGCTTTGTTTGTTGATAGATACAAAGATAAAGGCC10.641025641025641No Hit
GTTATGTTCTTTGCGAAAATAAAAGTTATTCCACAAACGCATTCGATAAAA10.641025641025641No Hit
TACTATTTGTTGTCTTGTGTGATTTTTCGATGTGGATTTTAGCTATCTTCT10.641025641025641No Hit
CACGAATACATAACTGCAAAAGAGAGGTTCAATAATTCAAGAACATAAGTC10.641025641025641No Hit
CTCTTATGGCCATTTCTCTCCTCTCCTATTCGTTCACCTTCCACTTCCCGT10.641025641025641No Hit
TCCTTATTCAAAACTTTCCTCCAAACATAATCTGCAAAATGGGGACAGATT10.641025641025641No Hit
ATCTTCAGTCAAGGTACGAAGTAACTCGACTGAAAGAAGAGGGGCTTGCTG10.641025641025641No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGAA10.641025641025641Illumina PCR Primer Index 5 (96% over 28bp)
GGAACAGATCTAATCTAAGCAGAGAGAGAGAGAGCGAAAACAAATTCAAAA10.641025641025641No Hit
GTCGTGGAGGCGCGAACCCACTTAGGTTTAAAAACTGAGGGGATGAGTTGT10.641025641025641No Hit
CTGTTGTTACTATTTGTTGTCTTGTGTGATTTTTCGATGTGGATTTTAGCT10.641025641025641No Hit
GTACATGACTTATCTTCTATATTTTCCTTTGACATTCTCCCTCTAAGGCCT10.641025641025641No Hit
ATCCCAGAGCATATCAAGGTAAGAAAGAGACTACTACATGTCTCTTTACAC10.641025641025641No Hit
ATATATCCCTTACTGCACACTTGATATGATATAGACCTGTCTCTTTACACA10.641025641025641No Hit
CTATTATCTCGTGTTTGTGTGAATAGGCTCGAAACTCTTACGACTGTCTCT10.641025641025641No Hit
GCCCTAGCGACCTCTATGCTAGGCTTGTAACGATGGTTACTTCAGGAGATT10.641025641025641No Hit
ATCTTACATTCACTACGTATTTGTCAAAAAGAATCATGATCATTTGCACAT10.641025641025641No Hit
CTGTGTTGTTCCTTTCCTTTGGGTCCAAGAAGACCTTCATGATTTTGGTAC10.641025641025641No Hit
GTGAATAGAGTAAACCCTCAAACAGTAGATAAGAAAGAAAACAAGTGCTGT10.641025641025641No Hit
GCAAAAGATCATCAATGGGAACCAAAATCCAAGTACTGTCTCTTATACACA10.641025641025641No Hit
GAAGAAAACAGTCAGTGCAGGTCTGAGGAACGTTTAATGGTGAGCTCGGCT10.641025641025641No Hit
CATTGTACGGATCCTTTAAAACCTTTTGGTGTTGTCTCTAATACTTGAAGT10.641025641025641No Hit
GTGCTCGTTCTCGTTACCAGAAGAGTTACCGTTAAACAAAGCAGGTTTGTG10.641025641025641No Hit
ACTAGGCATCATACCTTATGTTCTCTGCTATGAAACATATGATAATGGTTT10.641025641025641No Hit
ACATTAAAGATTGTGAACAAGGAACTGAATCAAGAGAGTTTCCTTCATCCT10.641025641025641No Hit
TGTATGTCTGATGAAGATTCAAAGAAGAGAGCTGCAAATGAAACCGTGTTT10.641025641025641No Hit
GCCCTGGCATGGGTTCATCACCAGGGAGATGACGTTTGCCCTATCCCAGGA10.641025641025641No Hit
ACACTGGTCGCAGTCGCCTTCAGGGTCGAAGCTCTGTCGCCAACGGCTGTC10.641025641025641No Hit
ATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGCTGTCTCTTAA10.641025641025641No Hit
TCTGAAAAGTGATTGTTTTTACAGCCTAAGTACATCAGCTACAGTTACAAG10.641025641025641No Hit
ACACTAAACCTTCTCATGGTCCTATTCATTTCCGTGCTCCGTCTAAGATCT10.641025641025641No Hit
CAAGTAATCACTAATAGCACTAGCACCAAACAAAGTCACATGTATACAAAG10.641025641025641No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGC10.641025641025641No Hit
GTTCATGAGACGAGTCCATATAAGAGTCTTGAAGAACTCTGGGACGGTGAG10.641025641025641No Hit
GCTAAGTACAAGGTTGGTGCCCCTGCTCGTGTGGGTTTGGTGGCTCCTGTC10.641025641025641No Hit
CTCTTCTAGAATGCAAATTTCCAACATTTGCATTCTTAGGTTTTGTAAGAT10.641025641025641No Hit
CCATTAGAGTCATCCCCGACTTCCCTAAACCAGGAATAATGTTTTCAGGAC10.641025641025641No Hit
TCATAATTCTTAGTTTTAAGTCTGCTACAAAACAAATGAAAAAGAGTTCTA10.641025641025641No Hit
CTTTTTATCACTCATGAAGTATTCTCCAGACTCCAACTGCATATCAATCTT10.641025641025641No Hit
CTTATGTCTGTTTACTTAGTTGCAGGTTTTGTGAAATGACTTTTCACATGT10.641025641025641No Hit
GCGCCTCGCGTCCGGTGCGCGTGGACGTCAGAATCGTCGCCGCGACCAACG10.641025641025641No Hit
GTTTTAAAATAAGGTTCTCATAAGCCATGTTTGACTTTAGGAGTTAGTACA10.641025641025641No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGC10.641025641025641No Hit
CTCTAAAACAGAGGAGTGCTTACAACAACAACAACTCCTGTCTCTTATAAC10.641025641025641No Hit
AAGGGAAGAAAATGACAGAAGAGAAAGAGTAGCTGTTGAGAAGGCAGAAAA10.641025641025641No Hit
CTCCCCTATGTGTGGCTTCGCCGCCGGAGAGAAACTCGGCAAGTCTCAATC10.641025641025641No Hit
CAGTAATCTTTTACTACGCTCTACCAGACATAAAAGGATTGCGTGAAAATA10.641025641025641No Hit
CTATTTACACTTGGCAGGAAAAAGGATACACAAAAGAGCTGTCTCTTATAC10.641025641025641No Hit
GTTCTGAGCTGACTGTTCGACGCCCGGGGAAAAGCTCCCGAGAGAGCCGTT10.641025641025641No Hit
GTATCAACGCAGAGTACGGGGACGTGAGAGAAGACGAAACACAAAAGGTGC10.641025641025641No Hit
AAAAGATCCTGTATGGAAGAAATTAAATGCTGTGAATCAACGTGTTGATAT10.641025641025641No Hit
GTATTTTTCTCTTCTTCCTCTTCTGAAAAATTAGGGTTTGAGTTCTTCTCC10.641025641025641No Hit
AAGATATTGTAGCTTAACAAAAAGCTACACATCACATGGGAACCTGTCTCT10.641025641025641No Hit
ACTTGAAGAAACTTACTTACGAGGAGAGGAAGAACAAGTTGATCGAGAGAG10.641025641025641No Hit
ATATAGCACTGAAGATACATCTTCGTTTAGTCTCTCAGAGATGTTTCTTGG10.641025641025641No Hit
GATTATAAGTGTTGCTTTGTTTTTAGTATGTTGGACCTCTGTCTCTTATAC10.641025641025641No Hit
GGTTATTTCTCTTTTGCTTTCTTTGATTGGATTAGTGGATTATGCATTTGG10.641025641025641No Hit
CCTTAAATAAGACCAAAAAGAAACAAGAAAAGTCCTTTTCAAGGAAGATCA10.641025641025641No Hit
ATAGAGAAAGGTGATTTGTCAACACTTGGTGTTGATTTTTTCGAGAAGTTT10.641025641025641No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers