##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_4_29.3410000000aeb0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3690 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.72384823848238 38.0 38.0 38.0 32.0 38.0 2 36.67669376693767 38.0 38.0 38.0 32.0 38.0 3 36.64769647696477 38.0 38.0 38.0 32.0 38.0 4 36.77289972899729 38.0 38.0 38.0 32.0 38.0 5 36.65555555555556 38.0 38.0 38.0 32.0 38.0 6 38.665853658536584 40.0 38.0 40.0 38.0 40.0 7 38.4289972899729 40.0 38.0 40.0 38.0 40.0 8 38.59647696476965 40.0 38.0 40.0 38.0 40.0 9 38.571273712737124 40.0 38.0 40.0 38.0 40.0 10 38.479674796747965 40.0 38.0 40.0 38.0 40.0 11 38.50162601626016 40.0 38.0 40.0 38.0 40.0 12 38.582926829268295 40.0 38.0 40.0 38.0 40.0 13 38.47940379403794 40.0 38.0 40.0 38.0 40.0 14 38.49647696476965 40.0 38.0 40.0 38.0 40.0 15 38.55907859078591 40.0 38.0 40.0 38.0 40.0 16 38.488617886178865 40.0 38.0 40.0 38.0 40.0 17 38.31029810298103 40.0 38.0 40.0 38.0 40.0 18 38.40975609756097 40.0 38.0 40.0 38.0 40.0 19 38.41463414634146 40.0 38.0 40.0 38.0 40.0 20 38.49918699186992 40.0 38.0 40.0 38.0 40.0 21 38.459349593495936 40.0 38.0 40.0 38.0 40.0 22 38.417886178861785 40.0 38.0 40.0 38.0 40.0 23 38.42520325203252 40.0 38.0 40.0 38.0 40.0 24 38.43224932249323 40.0 38.0 40.0 38.0 40.0 25 38.237127371273715 40.0 38.0 40.0 38.0 40.0 26 38.198644986449864 40.0 38.0 40.0 38.0 40.0 27 38.198373983739835 40.0 38.0 40.0 38.0 40.0 28 38.10271002710027 40.0 38.0 40.0 38.0 40.0 29 38.05528455284553 40.0 38.0 40.0 38.0 40.0 30 38.015989159891596 40.0 38.0 40.0 32.0 40.0 31 37.82439024390244 40.0 38.0 40.0 32.0 40.0 32 37.5940379403794 40.0 38.0 40.0 32.0 40.0 33 37.56233062330623 40.0 38.0 40.0 32.0 40.0 34 37.2869918699187 40.0 38.0 40.0 32.0 40.0 35 37.139024390243904 40.0 38.0 40.0 32.0 40.0 36 36.87588075880759 40.0 38.0 40.0 32.0 40.0 37 36.612737127371275 40.0 38.0 40.0 27.0 40.0 38 36.57018970189702 40.0 38.0 40.0 27.0 40.0 39 36.37940379403794 40.0 38.0 40.0 27.0 40.0 40 36.46449864498645 40.0 38.0 40.0 27.0 40.0 41 36.1940379403794 40.0 38.0 40.0 27.0 40.0 42 36.06775067750677 40.0 38.0 40.0 27.0 40.0 43 35.95040650406504 40.0 38.0 40.0 27.0 40.0 44 35.68075880758808 40.0 38.0 40.0 13.0 40.0 45 35.420054200542005 40.0 38.0 40.0 13.0 40.0 46 35.09078590785908 40.0 38.0 40.0 13.0 40.0 47 34.82249322493225 40.0 38.0 40.0 13.0 40.0 48 34.41138211382114 40.0 32.0 40.0 13.0 40.0 49 34.24146341463415 40.0 32.0 40.0 13.0 40.0 50 34.052032520325206 38.0 32.0 40.0 13.0 40.0 51 33.47669376693767 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.06702008928571246 2201 2 -6.0010974702380935 2201 3 1.4469308035714334 2201 4 1.3462239583333329 2201 5 -0.8706845238095156 2201 6 0.18718377976191647 2201 7 1.0922433035714434 2201 8 1.1016927083333243 2201 9 0.5353050595238145 2201 10 1.1883928571428655 2201 11 0.9962425595238074 2201 12 1.1573288690476247 2201 13 0.40665922619047024 2201 14 0.5643415178571445 2201 15 0.5902529761904702 2201 16 -0.21830357142856371 2201 17 0.9627046130952408 2201 18 -0.241443452380949 2201 19 0.38610491071428044 2201 20 0.6451450892857125 2201 21 0.5779203869047791 2201 22 -4.170386904761898 2201 23 0.9826264880952422 2201 24 0.7707589285714391 2201 25 0.7339471726190681 2201 26 1.077622767857143 2201 27 1.2513950892857224 2201 28 0.9420758928571615 2201 29 0.41281622023810627 2201 30 -0.012499999999995737 2201 31 0.3822730654761912 2201 32 2.4322730654761955 2201 33 1.2056733630952365 2201 34 1.4765438988095383 2201 35 2.1097284226190496 2201 36 2.9303571428571473 2201 37 3.1015066964285793 2201 38 2.556156994047626 2201 39 2.315736607142874 2201 40 2.204445684523826 2201 41 3.087165178571432 2201 42 3.2098214285714306 2201 43 2.3345796130952436 2201 44 3.2134858630952436 2201 45 3.6336867559523824 2201 46 3.9240141369047734 2201 47 4.284170386904776 2201 48 4.916889880952404 2201 49 4.441889880952388 2201 50 4.592206101190477 2201 51 5.591741071428579 1101 1 -1.5663132440476204 1101 2 -0.4344308035714306 1101 3 0.44693080357143344 1101 4 0.34622395833333286 1101 5 0.529315476190483 1101 6 -0.279482886904745 1101 7 -0.17442336309522233 1101 8 -1.998307291666677 1101 9 0.6686383928571473 1101 10 0.9217261904761997 1101 11 1.0629092261904702 1101 12 0.5573288690476232 1101 13 -0.06000744047619122 1101 14 -1.1356584821428584 1101 15 -0.6097470238095326 1101 16 -1.451636904761898 1101 17 -0.8039620535714249 1101 18 -0.8081101190476119 1101 19 -0.9138950892857167 1101 20 -1.0548549107142904 1101 21 -0.788746279761888 1101 22 -0.037053571428565135 1101 23 -1.0507068452380963 1101 24 -3.2625744047618994 1101 25 -0.6327194940475991 1101 26 -0.2890438988095241 1101 27 0.651395089285721 1101 28 1.0754092261904944 1101 29 0.9461495535714448 1101 30 0.9875000000000043 1101 31 0.9822730654761926 1101 32 1.7656063988095312 1101 33 -0.327659970238102 1101 34 2.009877232142877 1101 35 0.5097284226190482 1101 36 0.09702380952381162 1101 37 1.1015066964285793 1101 38 2.2894903273809604 1101 39 2.449069940476207 1101 40 2.3377790178571587 1101 41 2.8204985119047663 1101 42 -1.2235119047619065 1101 43 1.3679129464285822 1101 44 2.2801525297619065 1101 45 1.2003534226190453 1101 46 2.9906808035714363 1101 47 2.684170386904775 1101 48 3.650223214285738 1101 49 3.9085565476190496 1101 50 3.79220610119048 1101 51 1.1584077380952422 2202 1 1.2670200892857153 2202 2 1.398902529761905 2202 3 1.4469308035714334 2202 4 1.3462239583333329 2202 5 0.3293154761904802 2202 6 1.3871837797619193 2202 7 1.492243303571442 2202 8 1.501692708333323 2202 9 1.3353050595238116 2202 10 1.588392857142864 2202 11 1.396242559523806 2202 12 1.5573288690476232 2202 13 1.606659226190473 2202 14 1.3643415178571416 2202 15 1.3902529761904674 2202 16 1.3816964285714377 2202 17 0.5627046130952422 2202 18 1.3585565476190524 2202 19 1.1861049107142847 2202 20 1.4451450892857096 2202 21 1.3779203869047763 2202 22 1.6296130952380992 2202 23 1.3826264880952408 2202 24 1.5707589285714363 2202 25 1.1339471726190666 2202 26 1.8776227678571402 2202 27 1.2513950892857224 2202 28 1.7420758928571587 2202 29 1.612816220238109 2202 30 1.9875000000000043 2202 31 1.9822730654761926 2202 32 2.4322730654761955 2202 33 2.0056733630952337 2202 34 2.676543898809541 2202 35 2.1097284226190496 2202 36 2.5303571428571487 2202 37 3.1015066964285793 2202 38 2.9561569940476247 2202 39 2.7157366071428726 2202 40 2.6044456845238244 2202 41 3.087165178571432 2202 42 3.2098214285714306 2202 43 3.5345796130952465 2202 44 3.6134858630952422 2202 45 3.233686755952384 2202 46 4.324014136904772 2202 47 -0.7158296130952237 2202 48 -0.08311011904759624 2202 49 -0.1581101190476133 2202 50 0.3922061011904816 2202 51 0.19174107142858077 1102 1 1.2670200892857153 1102 2 1.398902529761905 1102 3 1.4469308035714334 1102 4 1.3462239583333329 1102 5 0.029315476190483025 1102 6 0.3871837797619193 1102 7 -0.00775669642855803 1102 8 -1.748307291666677 1102 9 0.3353050595238116 1102 10 -5.661607142857136 1102 11 -1.853757440476194 1102 12 -5.192671130952377 1102 13 -0.8933407738095269 1102 14 0.36434151785714164 1102 15 0.3902529761904674 1102 16 -1.1183035714285623 1102 17 0.8627046130952394 1102 18 0.8585565476190524 1102 19 1.5861049107142833 1102 20 1.4451450892857096 1102 21 0.8779203869047763 1102 22 0.6296130952380992 1102 23 1.2826264880952394 1102 24 -5.679241071428564 1102 25 1.0339471726190652 1102 26 1.3776227678571402 1102 27 1.651395089285721 1102 28 1.2420758928571587 1102 29 1.112816220238109 1102 30 1.4875000000000043 1102 31 1.4822730654761926 1102 32 1.9322730654761955 1102 33 2.0056733630952337 1102 34 1.6765438988095411 1102 35 2.509728422619048 1102 36 2.4303571428571473 1102 37 3.1015066964285793 1102 38 2.9561569940476247 1102 39 1.6157366071428712 1102 40 2.504445684523823 1102 41 1.4871651785714306 1102 42 3.109821428571429 1102 43 3.0345796130952465 1102 44 2.6134858630952422 1102 45 3.033686755952381 1102 46 3.324014136904772 1102 47 4.184170386904775 1102 48 4.816889880952402 1102 49 5.241889880952385 1102 50 5.29220610119048 1102 51 5.491741071428578 2203 1 1.2670200892857153 2203 2 1.398902529761905 2203 3 1.4469308035714334 2203 4 1.3462239583333329 2203 5 0.3293154761904802 2203 6 0.9871837797619207 2203 7 1.492243303571442 2203 8 1.1016927083333243 2203 9 0.935305059523813 2203 10 0.7883928571428669 2203 11 0.9962425595238074 2203 12 1.1573288690476247 2203 13 1.2066592261904745 2203 14 0.5643415178571445 2203 15 0.9902529761904688 2203 16 0.9816964285714391 2203 17 0.9627046130952408 2203 18 0.9585565476190538 2203 19 0.7861049107142861 2203 20 1.045145089285711 2203 21 0.9779203869047777 2203 22 1.2296130952381006 2203 23 1.7826264880952394 2203 24 0.7707589285714391 2203 25 1.5339471726190652 2203 26 1.8776227678571402 2203 27 1.651395089285721 2203 28 1.7420758928571587 2203 29 1.612816220238109 2203 30 1.5875000000000057 2203 31 1.582273065476194 2203 32 2.032273065476197 2203 33 0.4056733630952323 2203 34 2.2765438988095426 2203 35 2.1097284226190496 2203 36 2.13035714285715 2203 37 3.1015066964285793 2203 38 2.556156994047626 2203 39 2.315736607142874 2203 40 2.204445684523826 2203 41 2.6871651785714334 2203 42 3.2098214285714306 2203 43 2.7345796130952493 2203 44 2.813485863095245 2203 45 3.233686755952384 2203 46 2.324014136904772 2203 47 4.284170386904776 2203 48 4.516889880952405 2203 49 4.841889880952387 2203 50 5.392206101190482 2203 51 5.191741071428581 1103 1 0.2670200892857153 1103 2 1.398902529761905 1103 3 1.4469308035714334 1103 4 1.3462239583333329 1103 5 1.529315476190483 1103 6 1.0538504464285836 1103 7 0.8255766369047777 1103 8 0.8350260416666586 1103 9 1.001971726190476 1103 10 1.2550595238095283 1103 11 0.7295758928571416 1103 12 1.2239955357142875 1103 13 0.9399925595238088 1103 14 0.6976748511904773 1103 15 0.7235863095238031 1103 16 1.3816964285714377 1103 17 1.0293712797619037 1103 18 1.0252232142857167 1103 19 0.919438244047619 1103 20 1.111811755952374 1103 21 0.711253720238112 1103 22 1.2962797619047635 1103 23 1.115959821428575 1103 24 -0.4292410714285637 1103 25 0.8672805059524009 1103 26 1.210956101190476 1103 27 1.3180617559523853 1103 28 1.0754092261904944 1103 29 1.2794828869047734 1103 30 1.32083333333334 1103 31 1.3156063988095283 1103 32 1.7656063988095312 1103 33 1.3390066964285694 1103 34 2.009877232142877 1103 35 1.8430617559523839 1103 36 2.5970238095238116 1103 37 -0.23182663690475636 1103 38 1.622823660714289 1103 39 2.115736607142871 1103 40 2.6711123511904873 1103 41 3.153831845238095 1103 42 3.2764880952380935 1103 43 2.201246279761911 1103 44 2.946819196428578 1103 45 3.7003534226190453 1103 46 2.9906808035714363 1103 47 3.350837053571439 1103 48 4.650223214285738 1103 49 4.575223214285721 1103 50 5.4588727678571445 1103 51 5.325074404761914 2204 1 0.06702008928571246 2204 2 1.398902529761905 2204 3 1.4469308035714334 2204 4 1.3462239583333329 2204 5 -0.8706845238095156 2204 6 0.18718377976191647 2204 7 0.6922433035714448 2204 8 1.1016927083333243 2204 9 0.5353050595238145 2204 10 1.1883928571428655 2204 11 0.1962425595238031 2204 12 1.1573288690476247 2204 13 0.8066592261904759 2204 14 0.1643415178571388 2204 15 0.5902529761904702 2204 16 0.9816964285714391 2204 17 0.5627046130952422 2204 18 0.9585565476190538 2204 19 1.5861049107142833 2204 20 1.045145089285711 2204 21 -0.6220796130952237 2204 22 0.4296130952380963 2204 23 -4.017373511904758 2204 24 0.7707589285714391 2204 25 1.1339471726190666 2204 26 1.4776227678571416 2204 27 1.2513950892857224 2204 28 0.9420758928571615 2204 29 1.2128162202381105 2204 30 -1.4124999999999943 2204 31 1.582273065476194 2204 32 2.032273065476197 2204 33 1.6056733630952351 2204 34 2.2765438988095426 2204 35 2.1097284226190496 2204 36 2.13035714285715 2204 37 1.9015066964285765 2204 38 -2.443843005952374 2204 39 -2.684263392857126 2204 40 -5.395554315476176 2204 41 0.08716517857143202 2204 42 -1.9901785714285722 2204 43 -2.6654203869047564 2204 44 -4.386514136904758 2204 45 -3.3663132440476176 2204 46 -1.8759858630952309 2204 47 1.6841703869047748 2204 48 3.716889880952401 2204 49 3.641889880952384 2204 50 3.3922061011904816 2204 51 -0.20825892857142492 2205 1 1.2670200892857153 2205 2 1.398902529761905 2205 3 0.2469308035714306 2205 4 0.14622395833333002 2205 5 -0.8706845238095156 2205 6 -0.6128162202380807 2205 7 0.6922433035714448 2205 8 -1.498307291666677 2205 9 -1.2646949404761898 2205 10 -1.0116071428571374 2205 11 -1.2037574404761955 2205 12 -3.8426711309523753 2205 13 1.2066592261904745 2205 14 0.9643415178571431 2205 15 -0.20974702380953403 2205 16 -4.4183035714285595 2205 17 -4.037295386904759 2205 18 -1.241443452380949 2205 19 -0.013895089285718143 2205 20 1.045145089285711 2205 21 -4.022079613095222 2205 22 -0.37038690476190084 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1216 45 3.033686755952381 1216 46 3.6573474702381077 1216 47 4.350837053571439 1216 48 4.650223214285738 1216 49 4.90855654761905 1216 50 3.79220610119048 1216 51 4.991741071428578 2206 1 -0.7329799107142847 2206 2 0.3989025297619051 2206 3 1.4469308035714334 2206 4 -1.6537760416666671 2206 5 1.529315476190483 2206 6 0.05385044642858361 2206 7 0.49224330357144197 2206 8 -0.8316406250000128 2206 9 0.6686383928571473 2206 10 -0.07827380952380025 2206 11 1.0629092261904702 2206 12 -0.10933779761904105 2206 13 -0.06000744047619122 2206 14 -0.6356584821428584 2206 15 0.7235863095238031 2206 16 -1.951636904761898 2206 17 0.3627046130952394 2206 18 1.0252232142857167 2206 19 0.5861049107142833 2206 20 -3.7215215773809547 2206 21 0.37792038690477625 2206 22 -0.7037202380952365 2206 23 0.7826264880952394 2206 24 -0.09590773809522801 2206 25 -0.9660528273809348 2206 26 -1.1223772321428598 2206 27 0.9847284226190567 2206 28 0.7420758928571587 2206 29 1.2794828869047734 2206 30 1.32083333333334 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1211 14 0.9643415178571431 1211 15 0.5902529761904702 1211 16 0.5816964285714405 1211 17 0.5627046130952422 1211 18 0.5585565476190553 1211 19 0.7861049107142861 1211 20 0.6451450892857125 1211 21 -1.0220796130952223 1211 22 0.829613095238102 1211 23 -0.21737351190476062 1211 24 0.7707589285714391 1211 25 0.33394717261906237 1211 26 1.077622767857143 1211 27 -0.348604910714279 1211 28 0.9420758928571615 1211 29 1.2128162202381105 1211 30 1.187500000000007 1211 31 -0.01772693452380736 1211 32 0.4322730654761955 1211 33 0.005673363095233697 1211 34 -3.123456101190456 1211 35 0.9097284226190467 1211 36 0.9303571428571473 1211 37 2.301506696428582 1211 38 -5.443843005952374 1211 39 -2.684263392857126 1211 40 -2.795554315476174 1211 41 -2.3128348214285666 1211 42 -2.190178571428568 1211 43 -1.865420386904752 1211 44 -2.186514136904755 1211 45 -2.966313244047619 1211 46 -1.4759858630952252 1211 47 1.6841703869047748 1211 48 -0.4831101190475948 1211 49 -0.5581101190476119 1211 50 1.5922061011904773 1211 51 5.191741071428581 1208 1 1.2670200892857153 1208 2 0.541759672619051 1208 3 0.5897879464285793 1208 4 1.3462239583333329 1208 5 0.6721726190476289 1208 6 0.2443266369047734 1208 7 0.9208147321428726 1208 8 1.2159784226190382 1208 9 1.049590773809527 1208 10 1.3026785714285793 1208 11 0.8248139880952365 1208 12 1.5573288690476232 1208 13 1.3209449404761884 1208 14 1.078627232142857 1208 15 0.2473958333333215 1208 16 0.8102678571428683 1208 17 0.5055617559523853 1208 18 -1.0700148809523782 1208 19 -2.8424665178571473 1208 20 0.016573660714279015 1208 21 1.0922061011904916 1208 22 1.0581845238095298 1208 23 1.21119791666667 1208 24 -0.14352678571427901 1208 25 -2.8946242559523654 1208 26 -2.2652343750000057 1208 27 -2.491462053571425 1208 28 -2.400781249999987 1208 29 -2.530040922619037 1208 30 -2.1553571428571416 1208 31 -2.1605840773809533 1208 32 -1.7105840773809504 1208 33 -0.1371837797619122 1208 34 -1.4663132440476048 1208 35 -1.6331287202380977 1208 36 -2.783928571428568 1208 37 -1.3270647321428513 1208 38 -0.32955729166666003 1208 39 -1.3128348214285595 1208 40 0.8615885416666771 1208 41 -0.6556919642857153 1208 42 -0.24732142857142492 1208 43 -0.6082775297618994 1208 44 -0.5293712797619037 1208 45 0.17654389880952692 1208 46 -0.10455729166665861 1208 47 0.8270275297619207 1208 48 1.4597470238095482 1208 49 1.3847470238095312 1208 50 1.6493489583333343 1208 51 1.5631696428571473 1209 1 -0.2329799107142847 1209 2 -0.1010974702380949 1209 3 0.6969308035714334 1209 4 -0.15377604166666714 1209 5 0.029315476190483025 1209 6 -1.1128162202380807 1209 7 0.742243303571442 1209 8 -0.2483072916666771 1209 9 0.5853050595238116 1209 10 1.338392857142864 1209 11 -0.10375744047619406 1209 12 0.30732886904762324 1209 13 1.106659226190473 1209 14 1.1143415178571416 1209 15 0.6402529761904674 1209 16 1.1316964285714377 1209 17 0.3627046130952394 1209 18 0.8585565476190524 1209 19 1.0861049107142833 1209 20 -0.4298549107142904 1209 21 0.6279203869047763 1209 22 0.8796130952380992 1209 23 1.2826264880952394 1209 24 1.0707589285714363 1209 25 0.7839471726190652 1209 26 0.8776227678571402 1209 27 0.901395089285721 1209 28 0.9920758928571587 1209 29 0.6128162202381091 1209 30 0.7375000000000043 1209 31 1.4822730654761926 1209 32 1.9322730654761955 1209 33 1.5056733630952337 1209 34 2.676543898809541 1209 35 2.509728422619048 1209 36 2.6803571428571473 1209 37 2.8515066964285793 1209 38 1.9561569940476247 1209 39 2.115736607142871 1209 40 2.504445684523823 1209 41 2.9871651785714306 1209 42 2.359821428571429 1209 43 3.0345796130952465 1209 44 2.8634858630952422 1209 45 3.533686755952381 1209 46 2.824014136904772 1209 47 1.0591703869047748 1209 48 3.1918898809524023 1209 49 1.6168898809523853 1209 50 3.91720610119048 1209 51 2.116741071428578 1214 1 0.5170200892857153 1214 2 1.398902529761905 1214 3 1.4469308035714334 1214 4 1.3462239583333329 1214 5 0.779315476190483 1214 6 0.8871837797619193 1214 7 1.242243303571442 1214 8 1.001692708333323 1214 9 1.3353050595238116 1214 10 1.588392857142864 1214 11 0.8962425595238059 1214 12 0.8073288690476232 1214 13 0.8566592261904731 1214 14 0.8643415178571416 1214 15 0.6402529761904674 1214 16 -0.11830357142856229 1214 17 1.1127046130952394 1214 18 1.1085565476190524 1214 19 1.3361049107142833 1214 20 1.4451450892857096 1214 21 1.1279203869047763 1214 22 1.6296130952380992 1214 23 1.7826264880952394 1214 24 -0.054241071428563714 1214 25 1.0339471726190652 1214 26 0.6276227678571402 1214 27 0.15139508928572099 1214 28 -1.6329241071428413 1214 29 0.3628162202381091 1214 30 -1.6374999999999957 1214 31 -1.8927269345238074 1214 32 -2.8177269345238045 1214 33 -0.1193266369047663 1214 34 -4.323456101190459 1214 35 -0.36527157738095184 1214 36 -2.3196428571428527 1214 37 -0.39849330357142065 1214 38 -2.5438430059523753 1214 39 0.9907366071428712 1214 40 -1.370554315476177 1214 41 -1.1378348214285694 1214 42 -0.7651785714285708 1214 43 -0.3404203869047535 1214 44 -3.1365141369047578 1214 45 -0.966313244047619 1214 46 -2.925985863095228 1214 47 -2.315829613095225 1214 48 -1.3081101190475977 1214 49 -0.008110119047614717 1214 50 3.16720610119048 1214 51 0.24174107142857792 1215 1 0.4098772321428612 1215 2 -0.3153831845238102 1215 3 -0.9816406249999972 1215 4 -1.2252046130952365 1215 5 -1.8992559523809476 1215 6 0.2443266369047734 1215 7 0.06367187500001137 1215 8 0.6445498511904688 1215 9 -2.8075520833333343 1215 10 0.15982142857143344 1215 11 -2.3180431547619094 1215 12 -1.5855282738095227 1215 13 -2.8219122023809575 1215 14 -0.34994419642857366 1215 15 0.5331101190476133 1215 16 -0.046874999999992895 1215 17 0.21984747023809348 1215 18 0.21569940476190652 1215 19 -0.6996093750000014 1215 20 -1.4119977678571445 1215 21 -1.7649367559523697 1215 22 0.48675595238095326 1215 23 0.3540550595238088 1215 24 -3.143526785714279 1215 25 0.3910900297619193 1215 26 -0.4080915178571445 1215 27 0.22282366071429038 1215 28 -1.4007812499999872 1215 29 0.4699590773809632 1215 30 0.5589285714285737 1215 31 0.8394159226190467 1215 32 0.4322730654761955 1215 33 -2.7086123511904816 1215 34 -0.46631324404760477 1215 35 -3.0617001488095212 1215 36 -2.0696428571428527 1215 37 -1.612779017857136 1215 38 -0.043843005952375336 1215 39 -1.5985491071428442 1215 40 -2.852697172619031 1215 41 -0.0842633928571388 1215 42 -2.247321428571425 1215 43 -3.893991815476184 1215 44 -2.243656994047612 1215 45 -3.2520275297619037 1215 46 0.7525855654762026 1215 47 1.2555989583333442 1215 48 1.1740327380952564 1215 49 -6.900967261904757 1215 50 -2.064936755952374 1215 51 -8.43683035714285 1212 1 -4.875837053571427 1212 2 -0.3153831845238102 1212 3 -3.8387834821428513 1212 4 -3.939490327380952 1212 5 -1.0421130952380864 1212 6 0.2443266369047734 1212 7 -4.936328124999989 1212 8 0.6445498511904688 1212 9 -1.5218377976190425 1212 10 -3.1258928571428513 1212 11 -1.8894717261904788 1212 12 -4.442671130952377 1212 13 -2.250483630952381 1212 14 -0.6356584821428584 1212 15 -4.181175595238102 1212 16 -4.618303571428562 1212 17 -1.4944382440476147 1212 18 -1.212872023809517 1212 19 -1.2710379464285708 1212 20 -3.840569196428575 1212 21 -4.76493675595237 1212 22 -0.37038690476190084 1212 23 -0.9316592261904759 1212 24 -1.5720982142857096 1212 25 -1.18033854166665 1212 26 -1.6938058035714292 1212 27 -4.205747767857133 1212 28 -1.4007812499999872 1212 29 0.1842447916666785 1212 30 -0.29821428571428044 1212 31 -1.5891555059523768 1212 32 -1.2820126488095198 1212 33 -0.8514694940476204 1212 34 -0.46631324404760477 1212 35 -3.0617001488095212 1212 36 -0.9267857142857068 1212 37 -5.469921874999994 1212 38 -2.6152715773809447 1212 39 -1.1699776785714135 1212 40 0.1473028273809689 1212 41 -1.2271205357142847 1212 42 1.4669642857142833 1212 43 1.8202938988095312 1212 44 3.042057291666673 1212 45 2.890829613095235 1212 46 2.6097284226190567 1212 47 2.39845610119049 1212 48 0.8883184523809717 1212 49 3.2418898809523853 1212 50 4.077920386904765 1212 51 4.848883928571432 1213 1 0.2670200892857153 1213 2 0.3989025297619051 1213 3 1.4469308035714334 1213 4 1.3462239583333329 1213 5 0.529315476190483 1213 6 1.0538504464285836 1213 7 1.1589099702381063 1213 8 1.501692708333323 1213 9 1.3353050595238116 1213 10 1.588392857142864 1213 11 0.06290922619047024 1213 12 0.890662202380959 1213 13 -0.5600074404761912 1213 14 0.6976748511904773 1213 15 0.7235863095238031 1213 16 1.048363095238102 1213 17 1.3627046130952394 1213 18 1.3585565476190524 1213 19 0.5861049107142833 1213 20 1.111811755952374 1213 21 0.711253720238112 1213 22 1.2962797619047635 1213 23 1.4492931547619037 1213 24 1.5707589285714363 1213 25 1.2006138392857295 1213 26 -2.62237723214286 1213 27 -3.1819382440476147 1213 28 -3.091257440476177 1213 29 -0.8871837797618909 1213 30 -2.5124999999999957 1213 31 -2.5177269345238074 1213 32 -2.40106026785714 1213 33 -2.4943266369047663 1213 34 -1.8234561011904589 1213 35 0.34306175595238386 1213 36 1.5970238095238116 1213 37 -1.3984933035714207 1213 38 1.622823660714289 1213 39 2.449069940476207 1213 40 1.3377790178571587 1213 41 -0.012834821428569398 1213 42 1.9431547619047649 1213 43 -1.2987537202380892 1213 44 -1.553180803571422 1213 45 -5.299646577380951 1213 46 -3.675985863095228 1213 47 -5.649162946428557 1213 48 -5.01644345238093 1213 49 -8.258110119047615 1213 50 -3.041127232142852 1213 51 -7.508258928571422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 2.0 20 1.0 21 2.0 22 2.0 23 3.0 24 7.0 25 10.0 26 14.0 27 34.0 28 53.0 29 68.0 30 82.0 31 114.0 32 104.0 33 134.0 34 135.0 35 177.0 36 200.0 37 294.0 38 470.0 39 1782.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41128594682583 16.196418882257188 11.964188822571893 27.42810634834509 2 12.899728997289973 22.11382113821138 47.34417344173442 17.642276422764226 3 18.211382113821138 29.67479674796748 36.829268292682926 15.284552845528454 4 9.078590785907858 24.037940379403793 37.20867208672087 29.67479674796748 5 13.685636856368562 33.03523035230352 39.94579945799458 13.333333333333334 6 23.441734417344172 42.62872628726287 17.642276422764226 16.287262872628727 7 21.165311653116532 30.650406504065042 24.119241192411923 24.065040650406505 8 22.005420054200542 37.5609756097561 22.276422764227643 18.157181571815716 9 23.65853658536585 14.119241192411923 23.902439024390244 38.31978319783198 10 16.720867208672086 32.6829268292683 28.72628726287263 21.86991869918699 11 24.661246612466126 22.926829268292686 36.7479674796748 15.663956639566395 12 21.056910569105693 23.306233062330623 31.842818428184284 23.794037940379404 13 23.712737127371277 20.18970189701897 39.21409214092141 16.883468834688347 14 18.80758807588076 19.83739837398374 31.43631436314363 29.91869918699187 15 16.422764227642276 25.420054200542 29.756097560975608 28.401084010840112 16 24.308943089430894 26.23306233062331 30.29810298102981 19.15989159891599 17 16.070460704607044 34.90514905149051 28.455284552845526 20.56910569105691 18 28.50948509485095 23.983739837398375 30.569105691056908 16.93766937669377 19 21.761517615176153 22.032520325203254 31.626016260162604 24.579945799457995 20 18.211382113821138 22.086720867208673 31.382113821138212 28.319783197831978 21 18.319783197831978 22.872628726287264 31.517615176151764 27.289972899728998 22 16.53116531165312 33.170731707317074 31.680216802168022 18.617886178861788 23 16.205962059620596 25.962059620596207 32.19512195121951 25.636856368563688 24 26.205962059620596 26.531165311653115 31.59891598915989 15.663956639566395 25 16.043360433604338 35.90785907859079 33.00813008130081 15.040650406504067 26 24.390243902439025 28.184281842818425 32.79132791327913 14.634146341463413 27 14.769647696476964 37.642276422764226 32.330623306233065 15.257452574525745 28 13.577235772357724 28.61788617886179 31.59891598915989 26.205962059620596 29 13.875338753387535 37.235772357723576 31.355013550135503 17.533875338753386 30 24.065040650406505 28.184281842818425 31.19241192411924 16.558265582655824 31 26.585365853658537 26.043360433604335 31.490514905149052 15.880758807588075 32 16.476964769647697 37.31707317073171 30.62330623306233 15.582655826558264 33 15.230352303523034 28.80758807588076 40.18970189701897 15.772357723577235 34 23.983739837398375 28.6449864498645 31.490514905149052 15.880758807588075 35 16.124661246612465 28.13008130081301 39.647696476964775 16.097560975609756 36 24.959349593495936 26.72086720867209 32.41192411924119 15.907859078590786 37 16.368563685636854 38.02168021680217 29.539295392953928 16.070460704607044 38 14.227642276422763 30.05420054200542 38.97018970189702 16.7479674796748 39 13.468834688346885 28.319783197831978 30.867208672086722 27.344173441734416 40 13.387533875338754 28.75338753387534 39.539295392953925 18.319783197831978 41 13.279132791327914 28.102981029810298 29.756097560975608 28.86178861788618 42 24.092140921409214 27.994579945799458 28.075880758807585 19.83739837398374 43 13.956639566395665 27.723577235772357 37.046070460704605 21.27371273712737 44 14.254742547425476 36.96476964769648 28.61788617886179 20.16260162601626 45 13.604336043360435 27.100271002710024 36.639566395663955 22.65582655826558 46 23.06233062330623 26.82926829268293 27.398373983739837 22.710027100271002 47 14.227642276422763 25.745257452574528 26.205962059620596 33.82113821138211 48 12.981029810298104 25.121951219512194 26.639566395663955 35.25745257452574 49 22.46612466124661 23.902439024390244 25.067750677506773 28.563685636856366 50 14.552845528455286 24.634146341463413 35.55555555555556 25.257452574525747 51 13.604336043360435 24.281842818428185 27.5609756097561 34.552845528455286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.0 8 1.0 9 3.0 10 5.0 11 5.5 12 6.0 13 22.5 14 39.0 15 53.5 16 68.0 17 75.0 18 82.0 19 86.0 20 90.0 21 102.0 22 114.0 23 111.0 24 108.0 25 118.0 26 111.5 27 95.0 28 92.0 29 89.0 30 117.5 31 146.0 32 186.0 33 226.0 34 224.5 35 223.0 36 202.5 37 182.0 38 184.5 39 187.0 40 178.0 41 169.0 42 145.5 43 122.0 44 130.0 45 138.0 46 154.5 47 171.0 48 162.5 49 154.0 50 174.0 51 194.0 52 363.0 53 532.0 54 325.5 55 119.0 56 102.5 57 86.0 58 72.5 59 59.0 60 48.5 61 38.0 62 40.0 63 42.0 64 29.5 65 17.0 66 18.0 67 19.0 68 17.0 69 15.0 70 11.5 71 8.0 72 8.0 73 8.0 74 6.5 75 4.0 76 3.0 77 2.0 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10840108401084012 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3690.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.40921409214093 #Duplication Level Percentage of deduplicated Percentage of total 1 95.60585885486019 77.8319783197832 2 2.829560585885486 4.607046070460704 3 0.6657789613848202 1.6260162601626018 4 0.2330226364846871 0.7588075880758808 5 0.09986684420772304 0.40650406504065045 6 0.13315579227696406 0.6504065040650406 7 0.06657789613848203 0.3794037940379404 8 0.0 0.0 9 0.06657789613848203 0.4878048780487805 >10 0.2663115845539281 3.8211382113821135 >50 0.0 0.0 >100 0.033288948069241014 9.43089430894309 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 348 9.43089430894309 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT 40 1.084010840108401 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 17 0.4607046070460705 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.43360433604336046 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.40650406504065045 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 14 0.3794037940379404 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG 13 0.3523035230352303 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.3523035230352303 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.3523035230352303 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.24390243902439024 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.24390243902439024 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 7 0.1897018970189702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.1897018970189702 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.16260162601626016 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 6 0.16260162601626016 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.16260162601626016 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 6 0.16260162601626016 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 5 0.13550135501355012 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 5 0.13550135501355012 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.13550135501355012 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 4 0.10840108401084012 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT 4 0.10840108401084012 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT 4 0.10840108401084012 No Hit GTATCAACGCAGAGTACGGGCTGTCTCTTATACACATCTCCGAGCCCACGA 4 0.10840108401084012 No Hit GGTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCC 4 0.10840108401084012 No Hit GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA 4 0.10840108401084012 No Hit ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 4 0.10840108401084012 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.21680216802168023 22 0.0 0.0 0.5962059620596206 23 0.0 0.0 1.2195121951219512 24 0.0 0.0 1.6531165311653115 25 0.0 0.0 1.897018970189702 26 0.0 0.0 2.168021680216802 27 0.0 0.0 2.3848238482384825 28 0.0 0.0 2.8184281842818426 29 0.0 0.0 3.5772357723577235 30 0.0 0.0 4.065040650406504 31 0.0 0.0 4.661246612466124 32 0.0 0.0 5.420054200542006 33 0.0 0.0 6.287262872628726 34 0.0 0.0 6.937669376693767 35 0.0 0.0 8.021680216802167 36 0.0 0.0 8.915989159891598 37 0.0 0.0 9.75609756097561 38 0.0 0.0 11.002710027100271 39 0.0 0.0 12.276422764227643 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 20 6.1485154E-4 45.0 45 GGATGTG 25 0.0018443214 36.0 30 GTATGCC 25 0.0018443214 36.0 42 ATCTCGT 25 0.0018443214 36.0 37 GCCCACG 25 0.0018443214 36.0 18 GACAGGA 25 0.0018443214 36.0 26 AGGATGT 25 0.0018443214 36.0 29 CCACGAG 25 0.0018443214 36.0 20 TGATCTC 25 0.0018443214 36.0 35 TATGCCG 25 0.0018443214 36.0 43 GATGTGA 25 0.0018443214 36.0 31 CACGAGA 25 0.0018443214 36.0 21 CCCACGA 25 0.0018443214 36.0 19 ATGTGAT 25 0.0018443214 36.0 32 TCTCGTA 25 0.0018443214 36.0 38 ACGAGAC 25 0.0018443214 36.0 22 GATCTCG 25 0.0018443214 36.0 36 ATGCCGT 25 0.0018443214 36.0 44 CGTATGC 25 0.0018443214 36.0 41 GTGATCT 25 0.0018443214 36.0 34 >>END_MODULE