FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_29.3410000000aeb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_29.3410000000aeb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3690
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC3489.43089430894309No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT401.084010840108401No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA170.4607046070460705No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.43360433604336046No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.40650406504065045No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA140.3794037940379404No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG130.3523035230352303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.3523035230352303No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.3523035230352303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.24390243902439024No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.24390243902439024No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA70.1897018970189702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1897018970189702No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.16260162601626016No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA60.16260162601626016No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA60.16260162601626016No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA60.16260162601626016No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA50.13550135501355012No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA50.13550135501355012No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.13550135501355012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT40.10840108401084012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT40.10840108401084012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT40.10840108401084012No Hit
GTATCAACGCAGAGTACGGGCTGTCTCTTATACACATCTCCGAGCCCACGA40.10840108401084012No Hit
GGTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCC40.10840108401084012No Hit
GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA40.10840108401084012No Hit
ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC40.10840108401084012No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC206.1485154E-445.045
GGATGTG250.001844321436.030
GTATGCC250.001844321436.042
ATCTCGT250.001844321436.037
GCCCACG250.001844321436.018
GACAGGA250.001844321436.026
AGGATGT250.001844321436.029
CCACGAG250.001844321436.020
TGATCTC250.001844321436.035
TATGCCG250.001844321436.043
GATGTGA250.001844321436.031
CACGAGA250.001844321436.021
CCCACGA250.001844321436.019
ATGTGAT250.001844321436.032
TCTCGTA250.001844321436.038
ACGAGAC250.001844321436.022
GATCTCG250.001844321436.036
ATGCCGT250.001844321436.044
CGTATGC250.001844321436.041
GTGATCT250.001844321436.034
Produced by FastQC (version 0.11.2)