FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_28.3410000000aea4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_28.3410000000aea4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC60.32017075773745995RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC50.26680896478121663RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC50.26680896478121663No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC30.16008537886872998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16008537886872998No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA30.16008537886872998No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAGC30.16008537886872998No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA30.16008537886872998No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGGC20.10672358591248667No Hit
CCATCGAGGTTGAACAAGTGACTCCAGTAGCAGCTGAGAACATCGAGGTGC20.10672358591248667No Hit
AATGGCCACCGTTGAGGTTGAACAAGTCACTCCAGTAGCAGTAGAGAACGT20.10672358591248667No Hit
CAAAAGAAGAAGAAAAAAACTCTTCAAGAACCAAATGCCAAAGGAACATAG20.10672358591248667No Hit
GAGGTGACCCAACAGCCGGAAGAATCCGTCCCCGCCGTGACAGAACAAAAA20.10672358591248667No Hit
CCACCAGAGTTTCCTCTGGCTTTACCCCGCTCAGGCATAGTTCACCATCTT20.10672358591248667No Hit
ACATAATAGAGAGATAAAAGACATCAAACATAACCAACATGAAATCAACTA20.10672358591248667No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC20.10672358591248667No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10672358591248667No Hit
CCTCTCTTTGAAGCATCGTTAATAATAATACAAGAAGAAGTGTGTAACCTG20.10672358591248667No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT20.10672358591248667No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10672358591248667No Hit
CCACCGGAGTTTCAACCGGAGCTTCTTCATCTTTCTTGATCTCCACAACCT20.10672358591248667No Hit
GTAAAAGATATTGTTTGTACCAACAATGGGTCTCAAGATGCCATCACCATG20.10672358591248667No Hit
GGTTGGTACCGTCACAGCAAACTTTAGGAAAAAGCATCACTGAGTAATAGT20.10672358591248667No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA20.10672358591248667No Hit
GCCTTTGGTGGTGGCACCTCGATGTTCTCAGCTGCTACTGGAGTCACTTGT20.10672358591248667No Hit
GATAAAGATAAAGGAATGGCGTCCCTGAAATTTCCGTTGGAGATTCTCGCC20.10672358591248667No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)