FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_27.3410000000ae97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_27.3410000000ae97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965945
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3300.037.49864245
GCCCACG3250.036.69098318
CCGAGCC3500.033.4273614
CGAGCCC3500.033.4273615
CCCACGA3550.032.95655419
CGAGACG3650.032.6700523
CGTATGC3700.032.22856541
TATGCCG3700.032.22856543
GAGCCCA3750.031.19887216
TCCGAGC3850.030.3885113
GTATGCC3950.030.18878242
ATGCCGT4100.029.63307444
AGCCCAC3950.029.61918317
CCACGAG4050.029.4433820
CTCCGAG4200.028.39182912
TCGTATG4950.024.54456940
CGGTCTA602.468979E-422.4991879
ACGAGAC5400.022.49918622
CTCGTAT5550.021.4857139
CACGAGA5550.021.4857121
Produced by FastQC (version 0.11.2)