FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_26.3410000000ae8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_26.3410000000ae8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246362
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC3370.13679057646877357RNA PCR Primer, Index 45 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG302.1588712E-644.99898518
TATGCCG356.220609E-638.5783943
TGCCGTC356.220609E-638.5783945
AGCCCAC356.2292875E-638.5705617
CCCACGA356.2292875E-638.5705619
CGAGCCC356.2292875E-638.5705615
CGTATGC401.555854E-533.74923741
GACGTTC401.555854E-533.74923726
CCGAGCC401.555854E-533.74923714
CCACGAG401.555854E-533.74923720
GTGTAGG352.8076625E-432.1551861
ATGCCGT453.475551E-530.00541544
TCCGAGC453.4803725E-529.99932513
AACCCAC300.0051400729.99932531
CGTTCAT453.4803725E-529.99932528
CGAGACG453.4803725E-529.99932523
ACCCACA300.0051400729.99932532
CTCCGAG507.137071E-526.99939212
CACGAGA507.137071E-526.99939221
GAGACGT507.137071E-526.99939224
Produced by FastQC (version 0.11.2)