FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_23.3410000000ae54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_23.3410000000ae54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT31.9736842105263157No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC21.3157894736842104RNA PCR Primer, Index 23 (95% over 21bp)
CGCAGGCAGATGCCGCGCGTCGTCCACCAGTGGGCGCGCAGCACCGAGCGG10.6578947368421052No Hit
GTAGCGGCGAGCGAACCCGGATCAGCCCAAACCGAAACGCTTGCGTTTCGG10.6578947368421052No Hit
GGATATGCTTATAAAGATGTATGGTAATGCCTGTCTCTTATACACATCTCC10.6578947368421052No Hit
GTTAGTAGCGGCGAGCGAGAGCGGATTTGGGATTTTAAGAAAAAGAAAGAC10.6578947368421052No Hit
ACTCTAGTCTGCCTTGGCAGCTGAAGCAGCAGCTTGACCTCTGAGACGACC10.6578947368421052No Hit
AGGTAACACTGAATGGAGGACCGAACCGACTTACGTTAAAAAGTGAGCGGA10.6578947368421052No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTACCCTCCTCCCACTTTAA10.6578947368421052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT10.6578947368421052No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGAAAACTTAGCGAA10.6578947368421052No Hit
GGCTTATGTTATCTCCTCTCTTTTTGTCCAACCTTATTAATTTTAGTTGTA10.6578947368421052No Hit
TCAACGCAGAGTACGGGTACATGTCTGATTCACCTACCTACTGAAAAAATC10.6578947368421052No Hit
TCACATCCTCATCAGTGAGACTTGTTTTCTTTTTGGTTGGTGATTGTTTCC10.6578947368421052No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTCTGCCGTC10.6578947368421052No Hit
GATATAACTAGACAAATATTAAGCTCTTTGAGTACCGAGAGTTAAAATCCA10.6578947368421052No Hit
ACATATTACAATGAATCAATAAGGAGTCAAAATCCCTGTCTCTTATCACAT10.6578947368421052No Hit
GAGAGATGTTGTATTAGGAGAAACATTTGGATGATGTACCATAGCTGTCTC10.6578947368421052No Hit
AAGCAGTGAGGTGTGAATTGAGTTAAATGCTTAGTTCTATAACATTGCTGT10.6578947368421052No Hit
ATTCCATTCCATTCCATTCGATTGCAATTGAGTTGATTCCATTCCATTCCA10.6578947368421052No Hit
GAAAAACTCTTCTTGCTTCATTATCTAAGGAGTAAAATTCGTAATCCAAGA10.6578947368421052No Hit
CATATCACTTGATTTTCACTTACTCAGTGTAATCCAGTGACTGTCTCTTAT10.6578947368421052No Hit
GTACGGGACTAACAAATACACCACACATCAACTCATATTCTGTCTCTTATA10.6578947368421052No Hit
CAGTTATCATTGTAACAATCACAGTGATCTATGATACCTGTCTCTTAACAC10.6578947368421052No Hit
TTATCACCCTCTACGATTGACCTTTCCAAGTCATTCCTCTAAATCTGTCTC10.6578947368421052No Hit
CTATTGCTTTAGTTGCTTTGAGATTTTAAGTTTTGTCATTTCGACACTACT10.6578947368421052No Hit
TTATATGAGTCACAAGGTTATTTGGCAAACAATAGTTATGTCTGTCTCTTA10.6578947368421052No Hit
TATCAACGCAGAGTACGGGGTTTTAGTTTCCACGATGACGTTATGGGAGAA10.6578947368421052No Hit
CCATTTAAGATCTTCGTCGTCTCCTGTGTTCTCATCCTGTCTCTTATACAC10.6578947368421052No Hit
AAATGATGGAATACAATGACATATACCCCGTACTCTGCGTTGATACCTGTC10.6578947368421052No Hit
AGTTACTTCGTACCTTGACTGAAGATTCCTAAAGCTGTCTCTTATACACAT10.6578947368421052No Hit
AATCGGTGGTGATAGATGGTAATTTGATTACTAGTCGTCAGCCTGAAGATA10.6578947368421052No Hit
TTCTCCTTCTCACACATATATCTCACAAAAAAGTAAGCATGAGGAAAATTA10.6578947368421052No Hit
TCTCTGGTTCTTGCCCAAGTAGAATCAAGGGCTTCTATCTGTCTCTTATAC10.6578947368421052No Hit
CACTATGTTCATGACCATGTATGGTGCGCACATTTTTCCCTGTCTCTTATA10.6578947368421052No Hit
ATTACCTTCTTTGATTCATCTTTCCAGATGATTCGTCTAACATGTTCTTTT10.6578947368421052No Hit
GTATAAATCATGGCAGATCACTTTTTCCATGAAACCCTTTCGTATTCGGCG10.6578947368421052No Hit
CACTCGGAAAATTGAAATAATAACATACGGACACTGTTTTTAATTACTGTC10.6578947368421052No Hit
AAACAAGATCAATGACAAAAATGCTGAAATCAAAGATGCTATAAAGACTGT10.6578947368421052No Hit
TTCCAAACCACTTCACCTAACAACAATGCGAGGGCGCGGCAATGGTAGTCA10.6578947368421052No Hit
GACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTG10.6578947368421052No Hit
GGCAGACGGCGCGCAACCTGACGATGGCGACGCTCATGAACGCATCGCACA10.6578947368421052No Hit
GAATGGAATGGAATAGAATAGAATGGAATCAACCCGAGTGGAATGGAATCT10.6578947368421052No Hit
GTACCGGAGTGGGTGAGAGTCCTGTAACTGTAAGCGTGGCACTGGTGTGGG10.6578947368421052No Hit
GTAATATACTACCCCCTTTGTCTACTCTAGTTATCTTCCATCACTGTCTCT10.6578947368421052No Hit
CAAAAAAACGGAATACGAAACAAAAGATTCTAACAAGTTCCTGAAGAATCA10.6578947368421052No Hit
GTTTCTATCTACTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAGG10.6578947368421052No Hit
AGGAGGGAGAGAGAAAGTCACAGAAATAAAGAGAAAAAAGAAAAAGGCCTG10.6578947368421052No Hit
CCTCAAGGGTGCTCCTACTGTTTGTATGCATACGGTTTCAGGATCTATTTC10.6578947368421052No Hit
GTGTTATGGCATATTTCTTTCTATTGGAAATCAAGCTGTCTCTTATACACA10.6578947368421052No Hit
ACATCATCAGATGGATAAAACTCTATGATGATTTAAGATACCTCTGTCTCT10.6578947368421052No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT10.6578947368421052No Hit
ACATAAGAAGAAACTGTTTTGTTGTTTTCTATCTTTTGGCAAGCAGAACAC10.6578947368421052No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6578947368421052No Hit
GAATGGAATTGAATGGAATGGAATGCAATGGAATGGAATGGAATGGAATGG10.6578947368421052No Hit
TTTTTAAGAAGGTGCTTATTTTTGTAATGGTTGGAATAGGTCATATTACTG10.6578947368421052No Hit
CACCCTATACGTCCACTGTTCGTGTTGGCAGAGTGCTGTGATTTTGGTAAA10.6578947368421052No Hit
GTGAGAATGCTGACATGAGTAACGATAAATCCTGTGACTGTCTCTTATACA10.6578947368421052No Hit
ACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTGTC10.6578947368421052No Hit
GCTCTAAGCTTTGTGTCTTTTTTCCCGACATGTTGATCCATATACCTGTCT10.6578947368421052No Hit
ACTTTGAACATATTCTCTAACGCTTTGAGAAGCTCGGGGTAGTTTTTGTAT10.6578947368421052No Hit
ATATGGAGGATGATGAAGAAGAAGAAGAAGAAGAGATTGATCATATGGAGG10.6578947368421052No Hit
CGCCAGTAGCGTAGGGCGACCGGGCCAGGCCGAGTCAGAAAGGCTTTGATG10.6578947368421052No Hit
CAGTAAATGTATTTTTCATTCATTCTCTTCTCTGTACACAGCTATCATCAC10.6578947368421052No Hit
TTTTTGGTACTGGTCCGCTATCGGTCATCAAGAAGTATTTAGGCTTATCGG10.6578947368421052No Hit
ATTCTAGGTGGAGTACCTAAACATTTATTCAAACTTGAAGAAACTGACTTA10.6578947368421052No Hit
TCCTTTTTCTTATGGATTTTTAGGAAAGGGGAGAGACTGTCTCTTATACAA10.6578947368421052No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAA10.6578947368421052No Hit
GACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCA10.6578947368421052No Hit
GAATAATACTATCGCTGTTCATTATAGCTACTCTCATAACCCTCAACACCC10.6578947368421052No Hit
ATTCGATACCGTTTTATGATTCCTTTCAATTCCATTTGATGTTGATTCCAT10.6578947368421052No Hit
CTATCTAGAGGCTTTTCTCGGCAGTGTGAAATCCTGTCTCTCATACACATC10.6578947368421052No Hit
GGTTCTTGAAGTACCTCTTTTTTTAGTTGAACCCTTTATTCTGTCTCTTAT10.6578947368421052No Hit
ATGTAGGGCCGCAGAAGCTGCTCTCGAAATGAAACGCCGAACGGTTCTATT10.6578947368421052No Hit
CTCAAAGCCTAAAAGTGCAAAACAAAACAACCACAGGGATGCTCAAGAGAG10.6578947368421052No Hit
CTATTAAAACTATCAACTACTCATCTCCTTTTTATCACTAATTCACGCCAT10.6578947368421052No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC10.6578947368421052RNA PCR Primer, Index 45 (95% over 21bp)
GGTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.6578947368421052No Hit
GGCTAGTACTAGGTAACATTGTAGAATTGGCTACAGATATGGTTGAGAAGA10.6578947368421052No Hit
ACCTAAGAGAGTCCCATCACATCTCAGGCACACAACCCGCGGATTTACCAA10.6578947368421052No Hit
TCCCAAAGTAGTCTACCTTAGTTTACACTCAAAGGTAGCACTTGTTGAAAC10.6578947368421052No Hit
AGATTCATACCAGATTTCTCGTGTCTGATATTACTCAGGTGTCGTCACGAT10.6578947368421052No Hit
ATTCCATTCCATTCCATTCCAATCCATGCCATTCCACTCGTGTTGATTCCA10.6578947368421052No Hit
AGCCGATAAAGAGTTTACTCTTCTTTCTCCGACCATGGAAATCTGCTGCCT10.6578947368421052No Hit
GTGTATGAGACAAATGTATGCATTTCGATTAGGGTCGTATTCTATGGCTGT10.6578947368421052No Hit
TGGTAATGCCTTATAAGTCAGGTTATACAGGCTGAGCAATACCATCAACTA10.6578947368421052No Hit
ACTGAAAAGTCTTTTGGCTCGATGCGCTTAACAAAAGGGAACTCTCTAGAT10.6578947368421052No Hit
TATCAATTCGTTACTCATGCCTGCATTCTCACTCGCACACAGTCCACGCCT10.6578947368421052No Hit
ACATTTATGAGTCTCTCCATAGTGGCGTTTCTATATATGCTGTCTCTTATA10.6578947368421052No Hit
ATTACCTTCTTTGATTCATCTTTCCAGATGCTGTCTCTTATACACATCACC10.6578947368421052No Hit
CTCTTTTAATATTGTAACCTTTTGAAATCTATATGTTGCTGTCTCTTATAC10.6578947368421052No Hit
GTAATCAGTCGATTGATTTACAGAGTTGAGAGTTTGGTTTTTAGTTAGTTA10.6578947368421052No Hit
GAATTGAATGGAATGGAATCCACCCGATTGAAATGGAATGGAATGGAATGG10.6578947368421052No Hit
GAATGGAAACAACCCGAGTGGAATGGAATGGAATGGAAAGGAATGGAATGG10.6578947368421052No Hit
CAAGAGAACAGGGAAAAACCAAAAATACCTCAAGGTCAAGGATATAGACTG10.6578947368421052No Hit
TCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTCTGTC10.6578947368421052No Hit
ATCTTTGTTGTTGTAAGATGTTATAAGGATCTCAAGCACCTATTATTCTGT10.6578947368421052No Hit
AACAGACCATGTTTAATGTCTTCTCAGAGATTTTTTATACCTGTCTCTTAT10.6578947368421052No Hit
AAGTAAAACATCTCAGTAACCCGAGGAAAAGACATCAATAGAGATTCCCCT10.6578947368421052No Hit
GGCGTGATCTTCATGACCTCGTCAGTGAGCTTGGGCAGGAGAATGTCAACA10.6578947368421052No Hit
GATATATTCTCTGAGCTTTTGGGAGGTCACGGCAGCTTCCGGCGTCGCTTT10.6578947368421052No Hit
ATCCCGAAATATGGATAAAAAAATCAACTTAACGAGAGTATGACCAGACAT10.6578947368421052No Hit
GACAGAGGTTTGCCAATAGGGTCTCAATTCACAGGTTGAGAACCACTGCTT10.6578947368421052No Hit
ATCTTCTATTGGTTGGTGGAAGAACGCTGGCTTTCTCTCATCTTCATCAAA10.6578947368421052No Hit
GGTTCAGGAGGTTGGATTTTCTTCTGAAATAGGTGACTTCTGGGTATAGTT10.6578947368421052No Hit
GATTAATAATCAGCTATGTTGTTCCAATGAATGTACCTGTCTCTTATACAC10.6578947368421052No Hit
ATTAAACATAATGACATAGTTGAGAACCTAGCTGTGTTCAATTAAGACCTG10.6578947368421052No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC10.6578947368421052No Hit
ATCCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCA10.6578947368421052No Hit
GTGTCGAGGTGAAGAAAAAGAAACAAGGTCCAAAGATTGTCAATTTCGTTG10.6578947368421052No Hit
CTTTTATGTTTTAACTCTCGCTGATCATTACTTACTAGTTACTATCTATTC10.6578947368421052No Hit
TAAGTACGTAGTACAACCAATTCAGCACACTCCTAAAGGACTTGAAAACAC10.6578947368421052No Hit
GCATAAAGACGCAGCTATAATCACCCGTACTCTGCGTTGATACCTGTCTCT10.6578947368421052No Hit
GTATCAACGCAGAGTACGGGGTGACGGCACCGCTTGACCGAATAAAGCTCC10.6578947368421052No Hit
TTGTAGCACTACCAGTGGTGAATATGACACTAGGAATCCTTAAATCCTTAG10.6578947368421052No Hit
CCTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAC10.6578947368421052No Hit
TGTCTGTGCAACCCCACATCCTTTTCCACTTAACAATTATTTTGGGACCTT10.6578947368421052No Hit
AGATGCACCTTATAATAAGTGGCAGTGTCGTATTATGTCTGTCTCTTATAC10.6578947368421052No Hit
AAGTTGATGAGAAGAATCATAAGTCTGTGCCTACGGTATCTCCTGTCTCTT10.6578947368421052No Hit
ATCCAGTGGGGAAATCTTGTTTGTTACTGTCATTCCCATTCTTTTCGTTTA10.6578947368421052No Hit
GAATTAAGAAGAATCTCATAACTAGAAAAACAATGAAGCTCTCTCAAGTCT10.6578947368421052No Hit
ATTACGCACTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTAGTTGTC10.6578947368421052No Hit
TCACTCTCCTTTCCCTAATATGAACCTTGAGTCATCAAAGCCTTTCTGACT10.6578947368421052No Hit
GTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCT10.6578947368421052No Hit
CAACAAGCTTGATAGTTAATACTATGCTCTGTTTTCCAGTTTCAGAACAAA10.6578947368421052No Hit
CCCGTACCCTAAACCGACACAGGTGATCAGGTAGAGAATACTAAGGCGTTC10.6578947368421052No Hit
ATGCCGACATGAGTAACGATAAAAGAAGTGAGAATCTTCTTCGCCGTAAGG10.6578947368421052No Hit
CTTAGTAACTTAGCTTATATCCTCTGGTGTGTTTATTATCTGTCTCTTATA10.6578947368421052No Hit
TTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTATGCCT10.6578947368421052No Hit
GAATGGAACGGAATGTAATGGAATGGAATGGAATGGAATCTGTCTCTTATA10.6578947368421052No Hit
GACTTTCTCTCGAACCATAGTAATTTTATTTGATCAGATCGTTGAATCGTT10.6578947368421052No Hit
GCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTC10.6578947368421052No Hit
CCCGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGACTGTCTCTTATACACA10.6578947368421052No Hit
CACAATCGAATGGAATATAGATTTAATACGAAAACAACTGTCTCTTATACA10.6578947368421052No Hit
CTTATACACATCTCCGAGCCCACGAGAATCTGGACCATCTCGTATGCCGTC10.6578947368421052No Hit
TCATAAACGTGGTAATGGTAAGTTTGATAATAAAAATAGACGATCAAAAGG10.6578947368421052No Hit
CCTTCATACATGTTGGTCTCATCTACCAAAGTCATGATCTCTGTATCTGTA10.6578947368421052No Hit
GGATAATATTTAGGATGTACGATTTGGCTGTCCAGTTACAGTGGGGCAATG10.6578947368421052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGTCC10.6578947368421052No Hit
GCGTGGGCATGTTGGCCAAGATCAGACGAATGCACCTGCGAGATGGACTGT10.6578947368421052No Hit
CATTCATACCATCAAATCCTAAAGCACACAACAACTGAAAAAACAAAGACC10.6578947368421052No Hit
GATGTTAGCTTAGAAGCAGCTATTCATTCAAAGAGTGCGTAATACTGTCTC10.6578947368421052No Hit
ATCCTACTTGATCATGATGGATGATTTTATTTATGTGTTCTTGGATTCGGT10.6578947368421052No Hit
GTGTGTATGCATGTAAGAGAGAGATGGAATGTGTTGTTTACTTTTGTGAAA10.6578947368421052No Hit
GTATCAACGCAGAGTACGGGACCTCAAAGAAAACATCGAAATCTTAGAGAA10.6578947368421052No Hit
TCCCATAAGTTTGATCCTATAGAATCTGACCCATTTTCTCATTGAGCGAAG10.6578947368421052No Hit
ATGGAATAGAATCATCCGGAATGGAATGGAATGGAATGGAATGCAAAGCAA10.6578947368421052No Hit
CTGAAAGATGTAGGAATTGTTTCTGACCACACAAAAGCACAGAACTATCTG10.6578947368421052No Hit
CTGTATTACCTAAGACAATAAATATGATTTCTGTCTCTTATAACATCTCCG10.6578947368421052No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers