FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_22.3410000000ae47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_22.3410000000ae47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920058
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC31440.3417175873694919No Hit
AAATAAGATTGTACCGATCAGGAAAGCAAAAAAAAATGTTATCTGAATTCT21100.22933336811374935No Hit
AGATTGTACCGATCAGGAAAGCAAAAAAAAATGTTATCTGAATTCTCCCTT20340.2210730193096522No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9350.10162402805040552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3050.041.3101845
CCTAGGA250.00210721535.998873
GTGCCTC401.5605652E-533.7489411
ATGCCGT3850.032.72624644
CGTATGC3900.032.30667541
GCCCACG4150.030.36049318
TATGCCG4150.030.36049343
CCGTGTA602.4187648E-729.9990613
GTATGCC4200.029.9990642
AGAGGGC300.005147520529.9990634
CCCACGA4300.029.30140519
ACGAGAC4300.029.30140522
CCACGAG4300.029.30140520
TCGTATG4350.028.96460740
CTGGACC4350.028.96460730
AGCCCAC4400.028.63546417
CTCTGGA4350.028.44738228
GGACCAT4550.028.18592832
GGGGACA406.166334E-428.12411717
ACTCTGG4400.028.12411727
Produced by FastQC (version 0.11.2)