FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_2.3410000000ac49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_2.3410000000ac49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAACTTGAGAAGAGAGATACGATTCTTTTAGGAGTTTTAGTCCTGTCT17.6923076923076925No Hit
ATATGGAACATACGATGCAGCACACAACTTTATCTCTGAATGAAGAAAAAG17.6923076923076925No Hit
ATGTTGTACATTGATACTCTTGATGACTCGAGGAGGATTTATATGTACCTA17.6923076923076925No Hit
GAGTACGGGGGCTCTCTCGGGAGCTTTTCCCCGGGCGTCGAACAGTCAGCT17.6923076923076925No Hit
ATTTTGGAGCAAAAACGTTTTACAATACATTCATGTTGCCTGTCTCTTATA17.6923076923076925No Hit
GTACATCACTGTGGTCAACCATTACGTAGTCCCCGAATTTTGGACAATTCT17.6923076923076925No Hit
CTCTTTTGGTTTAGATAGTATAGAGATCATCAGATTGCTGAAACACTGTCT17.6923076923076925No Hit
GTAATATTCTTCTCCTCCTTCTGACAATATTTCATTCCATTTCTTGTCCAT17.6923076923076925No Hit
GTCAAAGATAGAAGACAAGTGAAAAATACAAGGCAACACTAGTAGATTTCT17.6923076923076925No Hit
GTTCATACGTAATAGTAGCTAACCCCACAAAATCCTGTCTCTTATAATCTC17.6923076923076925No Hit
GTTTTATGAAGGGACAGTGAAATCTTATGATTCCACAAAACAGAGACACTG17.6923076923076925No Hit
CTTCTATACGCAGACATTGTTGTGATATCGTATGCCGTCTTCTGCTTGAAA17.6923076923076925TruSeq Adapter, Index 5 (96% over 27bp)
CATCCAAAAAGACCTTCATGATCTTGGAACCATCAAGACCTGTCTCTTATA17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)