FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_19.3410000000ae10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_19.3410000000ae10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797368
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC50400.28041002176515883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6650.041.2766145
ATGCCGT7550.036.65422444
CTACCGC7750.035.41828
CCACGAG7850.034.39358520
GTATGCC8150.033.6796942
CACGAGA8150.033.4036321
TATGCCG8300.033.34209443
ATCTCGT8250.033.27145437
GCCCACG8200.033.19994718
ACTACCG8350.032.8729927
TACCGCT8450.032.75022529
CCCACGA8450.032.48396319
CCGCTGA8550.032.3671831
GAGACTA8500.032.2928824
ACCGCTG8600.032.17899730
TCTCGTA8600.032.17899738
TCGTATG8800.031.44765940
GACTACC8750.031.37022826
CGCTGAT8850.031.26998732
CGTATGC8850.031.0157641
Produced by FastQC (version 0.11.2)