FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_18.3410000000ae03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_18.3410000000ae03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869925
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC26000.2988763399143604RNA PCR Primer, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3100.037.74217645
GTTGGGC250.00210735635.9981616
GCCCACG3800.030.787918
TATGCCG3750.030.60019543
CGAGCCC3850.030.3880615
GGGTGCC300.00514786629.9984669
CGAGACC3850.029.80367323
CCCACGA4000.029.24850519
ATGCCGT4150.028.73512544
TTAGACG406.166772E-428.1235624
TCGATCG406.166772E-428.12356216
ACCTGTG4250.028.0573927
AGCCCAC4200.027.8557217
CCACGAG4200.027.32003220
GAGCCCA4300.027.20791216
GGCCTAT507.055916E-527.056161
CCGAGCC4350.026.89517814
CGTATGC4300.026.68468341
GTATGCC4300.026.68468342
GGACATC4300.026.68468333
Produced by FastQC (version 0.11.2)