FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_16.3410000000ade8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_16.3410000000ade8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC120.8456659619450317RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC100.7047216349541932No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT30.21141649048625794No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC30.21141649048625794No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC20.14094432699083861No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC20.14094432699083861No Hit
CTCTAAGCCAATCCCTCACGTTGTGCTTCTTCAAGTACTTCTTTGTCAAGT20.14094432699083861No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT20.14094432699083861No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC20.14094432699083861RNA PCR Primer, Index 45 (95% over 21bp)
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTCTG20.14094432699083861No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)