FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_15.3410000000addb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_15.3410000000addb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426060
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA25940.18189977981291108No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC22020.15441145533848505RNA PCR Primer, Index 23 (95% over 21bp)
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT19920.1396855672271854No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA19830.13905445773670114No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACGG250.002107713435.99848643
TGCCGTC2900.034.91232745
CGTATGC3200.032.3423941
CGAGACG3200.032.3423923
GCCCACG3250.031.84481418
GCGAGCA501.9827112E-631.49867620
TATGCCG3250.031.15253643
ATGCCGT3450.030.65088344
CGAGCCC3400.030.43989615
ATAGGGG751.7043931E-929.998743
CCCACGA3450.029.99873719
GGGGTGC300.005148729329.99873718
ACTACGC300.005148729329.9987378
GCGTGAG453.492431E-529.99873713
GATCTCG3500.029.57018536
GCTAGGA702.8883733E-828.9273552
CCGAGCC3800.028.41985714
CCACGAG3650.028.3549720
CCGGGTT406.168396E-428.12381628
AGCCCAC3800.027.82777617
Produced by FastQC (version 0.11.2)