FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_14.3410000000adce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_14.3410000000adce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189834
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC5230.2755038612682659RNA PCR Primer, Index 45 (95% over 21bp)
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2930.15434537543327329No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2870.1511847192810561No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA2060.10851586122612387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC700.038.57052645
AATTGCG250.002102930635.99915724
GTATAGG300.00511072530.0309431
GCCCACG901.2732926E-1129.99929818
CGTATGC901.2732926E-1129.99929841
ACGTTCA901.2732926E-1129.99929827
CGAGACG901.2732926E-1129.99929823
ATGCCGT901.2732926E-1129.99929844
TATGCCG952.3646862E-1128.4203943
CGTTCAT952.3646862E-1128.4203928
CCCACGA952.3646862E-1128.4203919
GTATGCC952.3646862E-1128.4203942
GAGCCCA952.3646862E-1128.4203916
AGCCCAC1004.5474735E-1126.99936917
CCGAGCC1004.5474735E-1126.99936914
CGAGCCC1004.5474735E-1126.99936915
TCCGAGC1058.367351E-1125.71368213
GAGACGT1058.367351E-1125.71368224
CCACGAG1058.367351E-1125.71368220
GCTTTAC450.001215453325.0257851
Produced by FastQC (version 0.11.2)