FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_13.3410000000ada5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_13.3410000000ada5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585350
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC17920.30614162466900147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.12471171094217134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2250.030.99858918
CCCTAGA2250.030.99858928
CGAGCCC2300.030.32470315
GCACTGC300.005146364729.99863411
ACCCTAG2350.029.67949927
CCTAGAA2450.029.38641729
GTATGCC2300.029.34648742
CCCACGA2500.028.79868919
TATGCCG2350.028.72209543
GACCCTA2450.028.46809226
ATGCCGT2400.028.1237244
CTAGAAT2500.027.8987330
TCCGAGC2500.027.8987313
TATCTCG2500.027.8987336
CCGAGCC2550.027.35169614
CGTATGC2500.026.9987741
GGTATCA5500.026.644321
GAGACCC2750.025.36247824
TGCCGTC2800.024.9095845
CCACGAG2850.024.4725720
Produced by FastQC (version 0.11.2)