FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_11.3410000000ad61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_11.3410000000ad61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173342
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC22141.2772438301161866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.3086384142331345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.20825881782833935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.1678762215735367No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT2910.1678762215735367No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1890.10903300988796714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2850.036.31369445
GTATGCC3300.032.04360642
AGCCCAC3250.031.84431517
GAGACTA3250.031.84431524
TCGTATG3250.031.84431540
ATGCCGT3250.031.84431544
CGTATGC3350.031.56534241
TATGCCG3350.031.56534243
ACTAAGC3300.031.36182827
TCCGAGC3400.031.10114713
CCCACGA3400.031.10114719
CCGAGCC3450.030.65040414
ACGAGAC3450.030.65040422
ATGATCT3400.030.4394234
CGAGACT3400.030.4394223
GACTAAG3400.030.4394226
AGACTAA3450.029.99826825
CTCGTAT3450.029.99826839
ACTAGGG300.005136794429.9982683
CGAGCCC3450.029.99826815
Produced by FastQC (version 0.11.2)