##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_4_10.3410000000ad48.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51084 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.939041578576465 38.0 38.0 38.0 32.0 38.0 2 36.93990290501918 38.0 38.0 38.0 32.0 38.0 3 36.94610837052698 38.0 38.0 38.0 32.0 38.0 4 37.03688043222927 38.0 38.0 38.0 32.0 38.0 5 37.02930467465351 38.0 38.0 38.0 32.0 38.0 6 38.979641374990216 40.0 38.0 40.0 38.0 40.0 7 38.91615770104142 40.0 38.0 40.0 38.0 40.0 8 38.84570511314698 40.0 38.0 40.0 38.0 40.0 9 38.86038681387519 40.0 38.0 40.0 38.0 40.0 10 38.831551953644976 40.0 38.0 40.0 38.0 40.0 11 38.89605355884426 40.0 38.0 40.0 38.0 40.0 12 38.81074308981286 40.0 38.0 40.0 38.0 40.0 13 38.85490564560332 40.0 38.0 40.0 38.0 40.0 14 38.81156526505364 40.0 38.0 40.0 38.0 40.0 15 38.83474277660324 40.0 38.0 40.0 38.0 40.0 16 38.819728290658524 40.0 38.0 40.0 38.0 40.0 17 38.83801190196539 40.0 38.0 40.0 38.0 40.0 18 38.87256283767912 40.0 38.0 40.0 38.0 40.0 19 38.86717954741211 40.0 38.0 40.0 38.0 40.0 20 38.80876595411479 40.0 38.0 40.0 38.0 40.0 21 38.85232166627515 40.0 38.0 40.0 38.0 40.0 22 38.8382076579751 40.0 38.0 40.0 38.0 40.0 23 38.82051131469736 40.0 38.0 40.0 38.0 40.0 24 38.84723201002271 40.0 38.0 40.0 38.0 40.0 25 38.83339206013625 40.0 38.0 40.0 38.0 40.0 26 38.81636128729152 40.0 38.0 40.0 38.0 40.0 27 38.8080220812779 40.0 38.0 40.0 38.0 40.0 28 38.75369978858351 40.0 38.0 40.0 38.0 40.0 29 38.71403962101637 40.0 38.0 40.0 38.0 40.0 30 38.68804322292694 40.0 38.0 40.0 38.0 40.0 31 38.671364810899696 40.0 38.0 40.0 38.0 40.0 32 38.64274528228017 40.0 38.0 40.0 38.0 40.0 33 38.57015895387988 40.0 38.0 40.0 38.0 40.0 34 38.558159110484695 40.0 38.0 40.0 38.0 40.0 35 38.50250567692428 40.0 38.0 40.0 38.0 40.0 36 38.389985122543266 40.0 38.0 40.0 38.0 40.0 37 38.3398911596586 40.0 38.0 40.0 38.0 40.0 38 38.256322919113614 40.0 38.0 40.0 38.0 40.0 39 38.13336856941508 40.0 38.0 40.0 38.0 40.0 40 38.11790384464803 40.0 38.0 40.0 38.0 40.0 41 38.043183775741916 40.0 38.0 40.0 38.0 40.0 42 37.93698614047451 40.0 38.0 40.0 38.0 40.0 43 37.79148069845744 40.0 38.0 40.0 32.0 40.0 44 37.65415785764623 40.0 38.0 40.0 32.0 40.0 45 37.43958969540365 40.0 38.0 40.0 32.0 40.0 46 37.31268107430898 40.0 38.0 40.0 32.0 40.0 47 37.14597525644037 40.0 38.0 40.0 32.0 40.0 48 36.968189648422204 40.0 38.0 40.0 27.0 40.0 49 36.829437788740115 40.0 38.0 40.0 27.0 40.0 50 36.735005089656255 40.0 38.0 40.0 27.0 40.0 51 36.34803460966252 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.6916161496754896 2201 2 0.09228411099019951 2201 3 -0.09086326618830043 2201 4 0.26271155713791217 2201 5 0.6025951619330598 2201 6 0.2880858470821366 2201 7 0.3258450220725706 2201 8 0.20542962480466542 2201 9 0.6141795070896237 2201 10 0.09737385755860828 2201 11 -0.07875677344154042 2201 12 0.040167931657862255 2201 13 -0.2837040275552951 2201 14 0.2884960731673303 2201 15 -0.045785799749893386 2201 16 0.023781543097967983 2201 17 0.5390828705701125 2201 18 -0.35137854026832827 2201 19 0.26389747674083 2201 20 0.4842498837392242 2201 21 -0.6008823637912499 2201 22 0.37090392580550713 2201 23 0.4699084412916932 2201 24 -0.0722037971653009 2201 25 -0.16921403499113552 2201 26 -0.4799349034002063 2201 27 -0.2796290591823194 2201 28 -0.3093856349195576 2201 29 0.11216478398891638 2201 30 0.21365911958788786 2201 31 -0.43129531763031537 2201 32 0.014410294578503624 2201 33 0.105065829292883 2201 34 0.3319439133504929 2201 35 -0.18989922793917202 2201 36 0.21724567913042847 2201 37 -0.5119758936113286 2201 38 -0.17487729608655656 2201 39 0.237276816718186 2201 40 0.4310558080600373 2201 41 0.12798193425815185 2201 42 0.7534531587257476 2201 43 0.005499068958741304 2201 44 0.3469853117814665 2201 45 0.3154004004677162 2201 46 0.09419117501196439 2201 47 -0.4922578490764806 2201 48 -0.36897789896264044 2201 49 0.037992771998311525 2201 50 0.33151095882178083 2201 51 -0.6076904692647744 1101 1 0.13546641705517004 1101 2 0.3204481038600733 1101 3 0.4225057177689209 1101 4 0.05950300098817962 1101 5 -0.14785047086551373 1101 6 -0.05014944703550839 1101 7 -0.7869000759666491 1101 8 -0.1706844571917685 1101 9 0.06917059443365048 1101 10 -0.43337480554299646 1101 11 -0.46066408181943785 1101 12 0.3561215858468074 1101 13 0.1597006070258118 1101 14 0.12539446888925454 1101 15 -0.24676619190675808 1101 16 0.24793484078067962 1101 17 -0.20601516864557823 1101 18 -0.2123411071132466 1101 19 0.14847858191017593 1101 20 -0.2617394210736137 1101 21 0.2422548911107114 1101 22 -0.347456145495741 1101 23 -0.31975287778139005 1101 24 0.2718247233338076 1101 25 0.18684657106947355 1101 26 0.015163135815484452 1101 27 0.2520108695164325 1101 28 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2202 49 0.0012280661159564943 2202 50 -0.13254132875992042 2202 51 -0.6395532143628131 1102 1 -0.18044267385392487 1102 2 -0.34962765371568594 1102 3 -0.16953973677653522 1102 4 0.06859391007908755 1102 5 -0.04519895571399957 1102 6 0.1373505529644916 1102 7 -0.40356674263331627 1102 8 0.017194330687019033 1102 9 -0.6380263752633155 1102 10 -0.1629202600884483 1102 11 -0.23831559697095628 1102 12 -0.1738026565774362 1102 13 -0.4035569687317633 1102 14 -0.21665098565620156 1102 15 -0.2009328585734238 1102 16 0.16422271956855639 1102 17 0.20231816468776032 1102 18 0.1353861656140296 1102 19 -0.16477899384739914 1102 20 -0.5356030574372497 1102 21 -0.28691177555595004 1102 22 -1.1357137212533175 1102 23 -0.43376802929653735 1102 24 -0.6876449736358907 1102 25 -0.006714034991134099 1102 26 -0.06817019751785125 1102 27 0.32625329375885315 1102 28 0.06340848272750321 1102 29 -0.5768058042463835 1102 30 -0.8091349980591716 1102 31 0.23267527060497883 1102 32 -0.6855897054214992 1102 33 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203.0 28 263.0 29 365.0 30 471.0 31 678.0 32 855.0 33 1066.0 34 1339.0 35 1742.0 36 2253.0 37 3392.0 38 6038.0 39 32111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68731621250735 18.806116447755343 14.126641834934325 22.37992550480298 2 15.388379923263646 24.197400360191057 40.20045415394253 20.213765562602774 3 17.614125753660637 29.054106961083704 32.092240231775115 21.239527053480543 4 11.919583431211338 18.761255970558295 38.98285177354945 30.33630882468092 5 13.432777386265759 37.91989664082687 32.779343825855456 15.867982147051915 6 26.548430036802127 41.10288935870331 16.15378592122778 16.194894683266774 7 24.720068906115415 35.38681387518597 21.88747944561898 18.005637773079634 8 25.642079711847153 33.21000704721635 20.57004150027406 20.577871740662438 9 27.689687573408506 15.235690235690235 20.470205935322213 36.604416255579046 10 17.32049173909639 28.505990133897114 29.257693211181586 24.915824915824917 11 31.399263957403495 26.04533709184872 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26.74810116670582 31.08018166157701 20.46824837522512 25 20.701198026779423 28.30827656409052 30.909873933129745 20.080651476000313 26 20.76383994988646 27.450865241562916 31.187847466917233 20.597447341633387 27 20.440842533865787 28.351342886226604 31.01362461827578 20.19418996163182 28 20.18635972124344 27.23161851068828 31.11541774332472 21.46660402474356 29 20.7481794691097 27.79539581865163 31.262234750606844 20.19418996163182 30 20.113929997650928 27.86586798214705 30.28541226215645 21.734789758045572 31 20.26074700493305 26.77746456816224 31.732049173909637 21.229739252995067 32 20.785373110954506 27.045650301464253 31.16435674575209 21.004619841829143 33 20.667919505128808 27.650536371466604 31.236786469344608 20.44475765405998 34 20.149166079398636 28.447263330984264 30.986218776916452 20.417351812700648 35 20.076736355806123 28.079242032730406 30.59274919740036 21.25127241406311 36 20.333176728525565 27.95200062641923 30.275624461670976 21.43919818338423 37 19.90251350716467 29.03648892020985 30.49487119254561 20.56612638007987 38 19.252603554929138 27.59768224884504 31.902356902356903 21.24735729386892 39 19.64020045415394 27.568318847388618 31.017539738469974 21.773940959987474 40 19.927961788426902 27.006499099522358 32.082452431289646 20.983086680761097 41 19.53644976900791 27.084801503406155 31.506929762743717 21.87181896484222 42 20.11197243755383 27.31970871505755 30.747396445070862 21.820922402317752 43 18.947224179782317 27.053480541852636 32.090282671678025 21.909012606687025 44 18.553754600266227 28.06945423224493 31.105629942839247 22.271161224649596 45 18.226842064051365 27.429332080494873 31.732049173909637 22.611776681544125 46 19.6558609349307 27.480228643019338 30.53010727429332 22.333803147756637 47 18.44021611463472 27.25315167175632 30.635815519536454 23.670816694072506 48 17.706131078224104 27.668154412340456 30.598621877691645 24.027092631743795 49 18.820351193156238 27.539494548088406 30.644245639449526 22.995908619305837 50 17.841202724923654 27.687730013311405 30.96272805575131 23.508339206013627 51 17.966486571137736 27.670111972437557 29.655077910891865 24.70832354553285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14.0 1 15.5 2 17.0 3 32.5 4 48.0 5 59.0 6 70.0 7 73.5 8 77.0 9 91.5 10 106.0 11 181.0 12 256.0 13 435.5 14 615.0 15 764.5 16 914.0 17 917.5 18 921.0 19 840.0 20 759.0 21 763.5 22 768.0 23 811.5 24 855.0 25 929.0 26 1119.5 27 1236.0 28 1453.5 29 1671.0 30 1789.0 31 1907.0 32 2075.0 33 2243.0 34 2261.0 35 2279.0 36 2314.0 37 2349.0 38 2303.5 39 2258.0 40 2372.5 41 2487.0 42 2574.0 43 2661.0 44 2717.5 45 2774.0 46 2851.5 47 2929.0 48 2909.5 49 2890.0 50 2822.0 51 2754.0 52 2725.5 53 2697.0 54 2752.0 55 2807.0 56 2290.5 57 1774.0 58 1648.0 59 1522.0 60 1404.0 61 1286.0 62 1174.5 63 1063.0 64 932.5 65 802.0 66 714.5 67 627.0 68 553.0 69 479.0 70 421.5 71 364.0 72 332.0 73 300.0 74 247.0 75 165.0 76 136.0 77 102.5 78 69.0 79 54.5 80 40.0 81 31.5 82 23.0 83 22.0 84 21.0 85 15.5 86 10.0 87 8.0 88 6.0 89 4.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14485944718502858 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0019575600970949806 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 51084.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.95300245464617 #Duplication Level Percentage of deduplicated Percentage of total 1 89.49133795479295 73.34083839078717 2 7.446540393386688 12.20532686275682 3 1.7019449239784177 4.184384895974276 4 0.6227493132620991 2.0414470399359206 5 0.23238835199108207 0.9522461590578162 6 0.14890934842127465 0.7322140918013088 7 0.07857095873606879 0.4507388181912662 8 0.0554005685273764 0.36321943428102965 9 0.03811541690299252 0.2811305569509726 >10 0.16501909117050412 2.5116157203490506 >50 0.009511922382481379 0.5086425763620857 >100 0.007133817336039486 1.4284649016483544 >500 0.0023779391120131587 0.9997305519039632 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC 513 1.004228329809725 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.6636128729151985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 216 0.42283298097251587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.3484456972829066 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 85 0.1663926082530734 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.12528384621407876 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 56 0.10962336543731892 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.10962336543731892 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0019575600970949806 2 0.0 0.0 0.0019575600970949806 3 0.0 0.0 0.0019575600970949806 4 0.0 0.0 0.0019575600970949806 5 0.0 0.0 0.0019575600970949806 6 0.0 0.0 0.0019575600970949806 7 0.0 0.0 0.0019575600970949806 8 0.0 0.0 0.0019575600970949806 9 0.0 0.0 0.0019575600970949806 10 0.0 0.0 0.0019575600970949806 11 0.0 0.0 0.0019575600970949806 12 0.0 0.0 0.0019575600970949806 13 0.0 0.0 0.0019575600970949806 14 0.0 0.0 0.0019575600970949806 15 0.0 0.0 0.0019575600970949806 16 0.0 0.0 0.003915120194189961 17 0.0 0.0 0.003915120194189961 18 0.0 0.0 0.003915120194189961 19 0.0 0.0 0.003915120194189961 20 0.0 0.0 0.005872680291284942 21 0.0 0.0 0.009787800485474904 22 0.0 0.0 0.015660480776759845 23 0.0 0.0 0.01957560097094981 24 0.0 0.0 0.01957560097094981 25 0.0 0.0 0.02544828126223475 26 0.0 0.0 0.027405841359329733 27 0.0 0.0 0.02936340145642471 28 0.0 0.0 0.06459948320413436 29 0.0 0.0 0.16835016835016836 30 0.0 0.0 0.3778090987393313 31 0.0 0.0 0.7693211181583275 32 0.0 0.0 1.1530028971889437 33 0.0 0.0 1.5738783180643645 34 0.0 0.0 1.945814736512411 35 0.0 0.0 2.3373267559314073 36 0.0 0.0 2.8110562994283925 37 0.0 0.0 3.5470988959361054 38 0.0 0.0 4.61005402865868 39 0.0 0.0 5.69062720225511 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 50 2.0008883E-11 44.999023 45 CTCGTAT 55 5.820766E-11 40.9082 39 CACGAGA 55 5.820766E-11 40.9082 21 TCTCGTA 55 5.820766E-11 40.9082 38 CCGAGCC 55 5.820766E-11 40.9082 14 GGACCAT 55 5.820766E-11 40.9082 32 ATGCCGT 55 5.820766E-11 40.9082 44 ACGAGAC 55 5.820766E-11 40.9082 22 CCACGAG 55 5.820766E-11 40.9082 20 ATCTCGT 60 1.4733814E-10 37.499184 37 CTCCGAG 60 1.4733814E-10 37.499184 12 TCCGAGC 60 1.4733814E-10 37.499184 13 TGGACCA 60 1.4733814E-10 37.499184 31 TATGCCG 60 1.4733814E-10 37.499184 43 CCCACGA 60 1.4733814E-10 37.499184 19 GTATGCC 60 1.4733814E-10 37.499184 42 CGAGCCC 60 1.4733814E-10 37.499184 15 CGAGACT 60 1.4733814E-10 37.499184 23 CTGGACC 60 1.4733814E-10 37.499184 30 GCCCACG 70 2.0008883E-11 35.356373 18 >>END_MODULE