FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_10.3410000000ad48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_10.3410000000ad48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51084
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC5131.004228329809725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.6636128729151985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.42283298097251587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.3484456972829066No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA850.1663926082530734No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.12528384621407876No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA560.10962336543731892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.10962336543731892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC502.0008883E-1144.99902345
CTCGTAT555.820766E-1140.908239
CACGAGA555.820766E-1140.908221
TCTCGTA555.820766E-1140.908238
CCGAGCC555.820766E-1140.908214
GGACCAT555.820766E-1140.908232
ATGCCGT555.820766E-1140.908244
ACGAGAC555.820766E-1140.908222
CCACGAG555.820766E-1140.908220
ATCTCGT601.4733814E-1037.49918437
CTCCGAG601.4733814E-1037.49918412
TCCGAGC601.4733814E-1037.49918413
TGGACCA601.4733814E-1037.49918431
TATGCCG601.4733814E-1037.49918443
CCCACGA601.4733814E-1037.49918419
GTATGCC601.4733814E-1037.49918442
CGAGCCC601.4733814E-1037.49918415
CGAGACT601.4733814E-1037.49918423
CTGGACC601.4733814E-1037.49918430
GCCCACG702.0008883E-1135.35637318