FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_4_1.3410000000ac12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_4_1.3410000000ac12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356146
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC6600.18531725752921555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC900.034.99857741
GCCCACG900.032.4986818
CTCGTAT950.030.78822339
TATGCCG950.030.78822343
CCGGCCG300.00514356429.9987859
ATGCCGT1001.8189894E-1229.24881644
CCCACGA1053.6379788E-1227.8560119
GAGACCC1053.6379788E-1227.8560124
GTATGCC1053.6379788E-1227.8560142
CACGAGA1150.027.39019221
TATCTCG1200.026.24893836
GACCCTA1159.094947E-1225.43374826
CGAGCCC1159.094947E-1225.43374815
TCGTATG1301.8189894E-1224.22978640
CGAGACC1400.024.10616523
CCACGAG1400.024.10616520
GGTATCA2200.023.5647871
TGCCGTC1450.023.27491845
GTGTATA602.433262E-422.5402321
ACCCTAG1358.367351E-1121.66578727
Produced by FastQC (version 0.11.2)