##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_94.3410000000abea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.214285714285715 0.0 0.0 0.0 0.0 0.0 2 38.0 0.0 0.0 0.0 0.0 0.0 3 37.214285714285715 0.0 0.0 0.0 0.0 0.0 4 37.57142857142857 0.0 0.0 0.0 0.0 0.0 5 38.0 0.0 0.0 0.0 0.0 0.0 6 39.42857142857143 0.0 0.0 0.0 0.0 0.0 7 39.714285714285715 0.0 0.0 0.0 0.0 0.0 8 38.642857142857146 0.0 0.0 0.0 0.0 0.0 9 38.642857142857146 0.0 0.0 0.0 0.0 0.0 10 39.714285714285715 0.0 0.0 0.0 0.0 0.0 11 39.714285714285715 0.0 0.0 0.0 0.0 0.0 12 39.714285714285715 0.0 0.0 0.0 0.0 0.0 13 39.857142857142854 0.0 0.0 0.0 0.0 0.0 14 39.857142857142854 0.0 0.0 0.0 0.0 0.0 15 38.07142857142857 0.0 0.0 0.0 0.0 0.0 16 38.785714285714285 0.0 0.0 0.0 0.0 0.0 17 39.285714285714285 0.0 0.0 0.0 0.0 0.0 18 39.142857142857146 0.0 0.0 0.0 0.0 0.0 19 39.142857142857146 0.0 0.0 0.0 0.0 0.0 20 39.57142857142857 0.0 0.0 0.0 0.0 0.0 21 39.142857142857146 0.0 0.0 0.0 0.0 0.0 22 37.214285714285715 0.0 0.0 0.0 0.0 0.0 23 39.42857142857143 0.0 0.0 0.0 0.0 0.0 24 39.57142857142857 0.0 0.0 0.0 0.0 0.0 25 37.785714285714285 0.0 0.0 0.0 0.0 0.0 26 39.714285714285715 0.0 0.0 0.0 0.0 0.0 27 37.92857142857143 0.0 0.0 0.0 0.0 0.0 28 38.92857142857143 0.0 0.0 0.0 0.0 0.0 29 37.92857142857143 0.0 0.0 0.0 0.0 0.0 30 39.714285714285715 0.0 0.0 0.0 0.0 0.0 31 39.714285714285715 0.0 0.0 0.0 0.0 0.0 32 39.857142857142854 0.0 0.0 0.0 0.0 0.0 33 37.92857142857143 0.0 0.0 0.0 0.0 0.0 34 38.92857142857143 0.0 0.0 0.0 0.0 0.0 35 39.57142857142857 0.0 0.0 0.0 0.0 0.0 36 39.57142857142857 0.0 0.0 0.0 0.0 0.0 37 39.57142857142857 0.0 0.0 0.0 0.0 0.0 38 39.57142857142857 0.0 0.0 0.0 0.0 0.0 39 39.714285714285715 0.0 0.0 0.0 0.0 0.0 40 39.42857142857143 0.0 0.0 0.0 0.0 0.0 41 38.857142857142854 0.0 0.0 0.0 0.0 0.0 42 37.785714285714285 0.0 0.0 0.0 0.0 0.0 43 39.714285714285715 0.0 0.0 0.0 0.0 0.0 44 39.714285714285715 0.0 0.0 0.0 0.0 0.0 45 39.42857142857143 0.0 0.0 0.0 0.0 0.0 46 39.714285714285715 0.0 0.0 0.0 0.0 0.0 47 39.42857142857143 0.0 0.0 0.0 0.0 0.0 48 39.142857142857146 0.0 0.0 0.0 0.0 0.0 49 39.142857142857146 0.0 0.0 0.0 0.0 0.0 50 39.57142857142857 0.0 0.0 0.0 0.0 0.0 51 37.642857142857146 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2209 1 0.0 2209 2 0.0 2209 3 0.0 2209 4 0.0 2209 5 0.0 2209 6 0.0 2209 7 0.0 2209 8 0.0 2209 9 0.0 2209 10 0.0 2209 11 0.0 2209 12 0.0 2209 13 0.0 2209 14 0.0 2209 15 0.0 2209 16 0.0 2209 17 0.0 2209 18 0.0 2209 19 0.0 2209 20 0.0 2209 21 0.0 2209 22 0.0 2209 23 0.0 2209 24 0.0 2209 25 0.0 2209 26 0.0 2209 27 0.0 2209 28 0.0 2209 29 0.0 2209 30 0.0 2209 31 0.0 2209 32 0.0 2209 33 0.0 2209 34 0.0 2209 35 0.0 2209 36 0.0 2209 37 0.0 2209 38 0.0 2209 39 0.0 2209 40 0.0 2209 41 0.0 2209 42 0.0 2209 43 0.0 2209 44 0.0 2209 45 0.0 2209 46 0.0 2209 47 0.0 2209 48 0.0 2209 49 0.0 2209 50 0.0 2209 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 32 1.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 2.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.428571428571427 28.57142857142857 7.142857142857142 42.857142857142854 2 0.0 50.0 35.714285714285715 14.285714285714285 3 0.0 42.857142857142854 28.57142857142857 28.57142857142857 4 14.285714285714285 7.142857142857142 64.28571428571429 14.285714285714285 5 28.57142857142857 21.428571428571427 35.714285714285715 14.285714285714285 6 21.428571428571427 57.14285714285714 21.428571428571427 0.0 7 50.0 0.0 14.285714285714285 35.714285714285715 8 7.142857142857142 64.28571428571429 21.428571428571427 7.142857142857142 9 28.57142857142857 7.142857142857142 28.57142857142857 35.714285714285715 10 7.142857142857142 21.428571428571427 57.14285714285714 14.285714285714285 11 21.428571428571427 21.428571428571427 35.714285714285715 21.428571428571427 12 0.0 28.57142857142857 28.57142857142857 42.857142857142854 13 7.142857142857142 28.57142857142857 42.857142857142854 21.428571428571427 14 0.0 7.142857142857142 35.714285714285715 57.14285714285714 15 14.285714285714285 35.714285714285715 28.57142857142857 21.428571428571427 16 14.285714285714285 21.428571428571427 35.714285714285715 28.57142857142857 17 0.0 21.428571428571427 50.0 28.57142857142857 18 28.57142857142857 35.714285714285715 28.57142857142857 7.142857142857142 19 14.285714285714285 28.57142857142857 14.285714285714285 42.857142857142854 20 14.285714285714285 28.57142857142857 21.428571428571427 35.714285714285715 21 14.285714285714285 21.428571428571427 28.57142857142857 35.714285714285715 22 28.57142857142857 7.142857142857142 57.14285714285714 7.142857142857142 23 14.285714285714285 21.428571428571427 42.857142857142854 21.428571428571427 24 7.142857142857142 42.857142857142854 28.57142857142857 21.428571428571427 25 21.428571428571427 28.57142857142857 28.57142857142857 21.428571428571427 26 14.285714285714285 42.857142857142854 35.714285714285715 7.142857142857142 27 21.428571428571427 28.57142857142857 35.714285714285715 14.285714285714285 28 7.142857142857142 21.428571428571427 57.14285714285714 14.285714285714285 29 21.428571428571427 28.57142857142857 7.142857142857142 42.857142857142854 30 14.285714285714285 14.285714285714285 35.714285714285715 35.714285714285715 31 21.428571428571427 21.428571428571427 14.285714285714285 42.857142857142854 32 7.142857142857142 28.57142857142857 35.714285714285715 28.57142857142857 33 21.428571428571427 35.714285714285715 35.714285714285715 7.142857142857142 34 28.57142857142857 7.142857142857142 57.14285714285714 7.142857142857142 35 14.285714285714285 35.714285714285715 28.57142857142857 21.428571428571427 36 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 37 21.428571428571427 35.714285714285715 35.714285714285715 7.142857142857142 38 7.142857142857142 28.57142857142857 28.57142857142857 35.714285714285715 39 7.142857142857142 35.714285714285715 14.285714285714285 42.857142857142854 40 14.285714285714285 35.714285714285715 42.857142857142854 7.142857142857142 41 50.0 28.57142857142857 14.285714285714285 7.142857142857142 42 35.714285714285715 35.714285714285715 28.57142857142857 0.0 43 14.285714285714285 35.714285714285715 28.57142857142857 21.428571428571427 44 0.0 42.857142857142854 28.57142857142857 28.57142857142857 45 21.428571428571427 21.428571428571427 35.714285714285715 21.428571428571427 46 28.57142857142857 28.57142857142857 35.714285714285715 7.142857142857142 47 21.428571428571427 28.57142857142857 28.57142857142857 21.428571428571427 48 0.0 42.857142857142854 35.714285714285715 21.428571428571427 49 21.428571428571427 14.285714285714285 28.57142857142857 35.714285714285715 50 0.0 35.714285714285715 50.0 14.285714285714285 51 7.142857142857142 21.428571428571427 35.714285714285715 35.714285714285715 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.5 33 1.0 34 1.0 35 1.0 36 2.0 37 3.0 38 3.0 39 3.0 40 2.0 41 1.0 42 2.0 43 3.0 44 1.5 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.5 53 1.0 54 0.5 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 14.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCTTGGAGGGCTTACAACATTAAACAAACTAATCTCACTGGCTGTGTCAT 1 7.142857142857142 No Hit CTTCAAGACTTCTTCTCTACTCTCCAATGACTGTAAAACTTGCATTTTCTC 1 7.142857142857142 No Hit GTTTCATGTTCTGTTTCTTCCTTGTTTCGGAAATCTTTAAGATCTGTCTCT 1 7.142857142857142 No Hit CATTGGCACTAATTTCTACGCTCTCTCTCTCCTGTCTCTTATACACATCTC 1 7.142857142857142 No Hit CATTGTTTTCCACCTGTGCAATTATTGTATGCGTCTGTCTCTTATACACAT 1 7.142857142857142 No Hit AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATCTGTC 1 7.142857142857142 No Hit ACAACTGAAAAAACAAACACCTTTCTATCTTCTTTGTTTGGTTTGGCAATG 1 7.142857142857142 No Hit GTCTGACTCTTTCCCTTACAAGGAGATTGAGAATGGAACTGTCTCTTATAC 1 7.142857142857142 No Hit TACTATGCTCTCTACCTTTGTGTAAGTTTGAAATTTTCCAAAAAAAAAAAA 1 7.142857142857142 No Hit GCACTACTCTCTATGTTGATTTGTTTTCCCATTTGAGAGAGAGAGAGAGTA 1 7.142857142857142 No Hit ATCGGGGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATCTGTCTC 1 7.142857142857142 No Hit CAATTACAGTTCACAGCGCCCTTTAGGAATCTTCAGTCAAGGTACGAAGTA 1 7.142857142857142 No Hit CAATAAGACATCTCACTAATGGAAGAAACCGCCAATATAGGAACTGTCTCT 1 7.142857142857142 No Hit CAAGTACATTATACTTCTGTCTCAGATTCCCTAGTCACCCAGAATAACTAT 1 7.142857142857142 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 7.142857142857143 33 0.0 0.0 7.142857142857143 34 0.0 0.0 7.142857142857143 35 0.0 0.0 14.285714285714286 36 0.0 0.0 14.285714285714286 37 0.0 0.0 14.285714285714286 38 0.0 0.0 14.285714285714286 39 0.0 0.0 21.428571428571427 >>END_MODULE >>Kmer Content pass >>END_MODULE