FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_93.3410000000abdd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_93.3410000000abdd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATGTAGGCAACCCAAACAATGAAGTGACAGTGAGACAACTCTGTCT16.25No Hit
TGTTGCCTCTGTTATCGATACTGCTCTTAAAGGTTACGCTCGTGAAGGTCG16.25No Hit
GGGTGGTAGACTCCATCTAAAGCTAAATATCGGGGAGCTGTCTCTTATACA16.25No Hit
GCTTTTCCATTCTTCTCATATGTGCTTGAACGAGACGACTTGTATTTCTGT16.25No Hit
ACATATATCTATGTCGCATCTGGAGAAATATATGGTGGTGAAAAGACACTG16.25No Hit
TCTATACAATGATACGTTTACACACACTACACATACATCCTCTCATCACAC16.25No Hit
CCATGGAGATTATCCACCTCTTGTCTGACTTGAACCCAATTCAGGTCATCA16.25No Hit
AATGAAGACAGCTCCTATTTTTGCTCAGTTTTACAAAGGATCCCTCTGTTC16.25No Hit
CATGTTTTTAGAAAGATTCCTCAGACTCTCCCCAATCAATCTCCTTCTTCT16.25No Hit
GTCTGGTACTGGTTCGTTGAGAGTTGGGGCTGAGTTTTCTCAAAACACACT16.25No Hit
GGCTTACTACTACTCCTTCTCTCTTCACTTACCAGAAGCCCTACTTCAACA16.25No Hit
CATGTGAGCCTTCTTCTGCTTCAATCCCTTCATCTTCCTGATCTGCTGTCT16.25No Hit
CGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTAC16.25No Hit
GTGGAGTACCTAAACATTTATTCAAACTTGAAGAAACTGACTTAACACTTT16.25No Hit
AGGTTGCTTCATTCCTTGTCATGTGCTAAGTACAAGATCTGTCTCTTATAC16.25No Hit
AAATCTCCCTTACTTGCAAGCAATAGTTAAACTGTCTCTTATACACATCTC16.25No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)