##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_90.3410000000aba7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.0 0.0 0.0 0.0 0.0 0.0 2 38.0 0.0 0.0 0.0 0.0 0.0 3 37.142857142857146 0.0 0.0 0.0 0.0 0.0 4 36.285714285714285 0.0 0.0 0.0 0.0 0.0 5 36.285714285714285 0.0 0.0 0.0 0.0 0.0 6 39.142857142857146 0.0 0.0 0.0 0.0 0.0 7 39.42857142857143 0.0 0.0 0.0 0.0 0.0 8 39.714285714285715 0.0 0.0 0.0 0.0 0.0 9 39.714285714285715 0.0 0.0 0.0 0.0 0.0 10 40.0 0.0 0.0 0.0 0.0 0.0 11 39.714285714285715 0.0 0.0 0.0 0.0 0.0 12 39.714285714285715 0.0 0.0 0.0 0.0 0.0 13 40.0 0.0 0.0 0.0 0.0 0.0 14 39.714285714285715 0.0 0.0 0.0 0.0 0.0 15 39.714285714285715 0.0 0.0 0.0 0.0 0.0 16 39.42857142857143 0.0 0.0 0.0 0.0 0.0 17 40.0 0.0 0.0 0.0 0.0 0.0 18 39.142857142857146 0.0 0.0 0.0 0.0 0.0 19 39.714285714285715 0.0 0.0 0.0 0.0 0.0 20 40.0 0.0 0.0 0.0 0.0 0.0 21 40.0 0.0 0.0 0.0 0.0 0.0 22 35.857142857142854 0.0 0.0 0.0 0.0 0.0 23 37.857142857142854 0.0 0.0 0.0 0.0 0.0 24 38.142857142857146 0.0 0.0 0.0 0.0 0.0 25 38.142857142857146 0.0 0.0 0.0 0.0 0.0 26 35.857142857142854 0.0 0.0 0.0 0.0 0.0 27 37.0 0.0 0.0 0.0 0.0 0.0 28 37.857142857142854 0.0 0.0 0.0 0.0 0.0 29 37.857142857142854 0.0 0.0 0.0 0.0 0.0 30 37.857142857142854 0.0 0.0 0.0 0.0 0.0 31 37.857142857142854 0.0 0.0 0.0 0.0 0.0 32 38.857142857142854 0.0 0.0 0.0 0.0 0.0 33 39.42857142857143 0.0 0.0 0.0 0.0 0.0 34 38.142857142857146 0.0 0.0 0.0 0.0 0.0 35 38.857142857142854 0.0 0.0 0.0 0.0 0.0 36 39.142857142857146 0.0 0.0 0.0 0.0 0.0 37 38.57142857142857 0.0 0.0 0.0 0.0 0.0 38 39.42857142857143 0.0 0.0 0.0 0.0 0.0 39 39.142857142857146 0.0 0.0 0.0 0.0 0.0 40 39.42857142857143 0.0 0.0 0.0 0.0 0.0 41 38.57142857142857 0.0 0.0 0.0 0.0 0.0 42 39.142857142857146 0.0 0.0 0.0 0.0 0.0 43 39.42857142857143 0.0 0.0 0.0 0.0 0.0 44 40.0 0.0 0.0 0.0 0.0 0.0 45 40.0 0.0 0.0 0.0 0.0 0.0 46 39.714285714285715 0.0 0.0 0.0 0.0 0.0 47 40.0 0.0 0.0 0.0 0.0 0.0 48 40.0 0.0 0.0 0.0 0.0 0.0 49 39.714285714285715 0.0 0.0 0.0 0.0 0.0 50 39.714285714285715 0.0 0.0 0.0 0.0 0.0 51 40.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 35 1.0 36 0.0 37 0.0 38 1.0 39 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.857142857142854 28.57142857142857 14.285714285714285 14.285714285714285 2 0.0 28.57142857142857 57.14285714285714 14.285714285714285 3 0.0 57.14285714285714 14.285714285714285 28.57142857142857 4 0.0 14.285714285714285 42.857142857142854 42.857142857142854 5 0.0 71.42857142857143 14.285714285714285 14.285714285714285 6 0.0 85.71428571428571 14.285714285714285 0.0 7 71.42857142857143 0.0 14.285714285714285 14.285714285714285 8 14.285714285714285 57.14285714285714 14.285714285714285 14.285714285714285 9 0.0 28.57142857142857 0.0 71.42857142857143 10 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 11 71.42857142857143 14.285714285714285 0.0 14.285714285714285 12 14.285714285714285 28.57142857142857 57.14285714285714 0.0 13 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 14 0.0 42.857142857142854 14.285714285714285 42.857142857142854 15 0.0 57.14285714285714 28.57142857142857 14.285714285714285 16 28.57142857142857 42.857142857142854 0.0 28.57142857142857 17 0.0 57.14285714285714 42.857142857142854 0.0 18 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 19 14.285714285714285 57.14285714285714 28.57142857142857 0.0 20 28.57142857142857 28.57142857142857 14.285714285714285 28.57142857142857 21 57.14285714285714 14.285714285714285 28.57142857142857 0.0 22 0.0 57.14285714285714 14.285714285714285 28.57142857142857 23 0.0 57.14285714285714 28.57142857142857 14.285714285714285 24 14.285714285714285 28.57142857142857 42.857142857142854 14.285714285714285 25 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 26 14.285714285714285 42.857142857142854 0.0 42.857142857142854 27 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 28 0.0 28.57142857142857 14.285714285714285 57.14285714285714 29 14.285714285714285 42.857142857142854 42.857142857142854 0.0 30 28.57142857142857 42.857142857142854 14.285714285714285 14.285714285714285 31 28.57142857142857 14.285714285714285 28.57142857142857 28.57142857142857 32 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 33 14.285714285714285 57.14285714285714 14.285714285714285 14.285714285714285 34 28.57142857142857 42.857142857142854 0.0 28.57142857142857 35 28.57142857142857 14.285714285714285 28.57142857142857 28.57142857142857 36 0.0 0.0 28.57142857142857 71.42857142857143 37 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 38 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 39 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 40 42.857142857142854 42.857142857142854 14.285714285714285 0.0 41 14.285714285714285 14.285714285714285 14.285714285714285 57.14285714285714 42 14.285714285714285 28.57142857142857 14.285714285714285 42.857142857142854 43 14.285714285714285 28.57142857142857 14.285714285714285 42.857142857142854 44 28.57142857142857 14.285714285714285 14.285714285714285 42.857142857142854 45 28.57142857142857 28.57142857142857 42.857142857142854 0.0 46 28.57142857142857 0.0 28.57142857142857 42.857142857142854 47 14.285714285714285 28.57142857142857 42.857142857142854 14.285714285714285 48 14.285714285714285 0.0 28.57142857142857 57.14285714285714 49 14.285714285714285 0.0 42.857142857142854 42.857142857142854 50 0.0 42.857142857142854 42.857142857142854 14.285714285714285 51 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.5 33 1.0 34 1.0 35 1.0 36 1.0 37 1.0 38 0.5 39 0.0 40 0.5 41 1.0 42 1.0 43 1.0 44 0.5 45 0.0 46 0.0 47 0.0 48 0.5 49 1.0 50 0.5 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.5 59 1.0 60 0.5 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 7.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAACAATACACAGCCCAATGGCTCTCTCTCTTTCTCTCTGTCCCTCTCTAC 1 14.285714285714285 No Hit GTATAAGAAGAGAAAGAGGTAAATGAGCTTCACGGCCATTCCTGTCTCTTA 1 14.285714285714285 No Hit TATTTAGACCGTTTTGTAACTCTTGAAAAATGAGATTGAGCTACTCTCCAA 1 14.285714285714285 No Hit ACACAAGCATGTTATATCAGGAATACTAAGGCACTTGAAACAACATGCTCT 1 14.285714285714285 No Hit CTACCAGACAGTCCACACACTTAGCCCCGGACAACCATCACCGGGGATGAG 1 14.285714285714285 No Hit ATCAATCTCAGAGAAAAAAAGAAATGGCAACAAACCCTAAAGCTGTCTCTT 1 14.285714285714285 No Hit GTCTAAGGCTGTTCAATTTAGACATAATTAGAGAGCACGGGACAAGAGCTG 1 14.285714285714285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE