FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_87.3410000000ab70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_87.3410000000ab70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGCTCATGGATTGGTAGGACTGACATAGTAAAAATGGCTGTCTCT12.941176470588235No Hit
CTCATATTCCCCTCTTAGCTCATATTTCTTGTGTTTGGAATGTTGTTAGTT12.941176470588235No Hit
GTATTATTTATGTCATCATCATCCTCCTCTTTTGTGTTATTGTTGGGTTCC12.941176470588235No Hit
GTACACAACTATGGATCAACAACAACAACAACCTCTGTCTCTTATACACAT12.941176470588235No Hit
TCACTAAGGTCAAGCAACTCTTGGAAGTTGCTGCTTCCAAGGCCTGTCTCT12.941176470588235No Hit
GGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGA12.941176470588235No Hit
CCTCTTGAGTGGTACTGTTTCCACCAAGATCTTCAGTCCGACAATTCCCTT12.941176470588235No Hit
TGACAGTACTGTTATCAGATTGGAGAGCAAGAACTCTAAAGCCTCTGACCT12.941176470588235No Hit
GGTTTAGGTATCGCATTAGTTTCAACTTCTGAAGGTGTTATCACTGATAAA12.941176470588235No Hit
ATTTAGGTCAAATCATATCGATCCGATCTTTATTAGTCCCTTGTGCATATG12.941176470588235No Hit
AGTTGAAGGTGTTAAAAAGTTTTCATAACAGTCAGAGGAAACATATTCACT12.941176470588235No Hit
ATGGTATTCTATATGCTCCAGCTGTTATGCTCTGTCTCTTATACACATCTC12.941176470588235No Hit
AGACAATTCTTCTCCTTCGATTGTGGTCCAGGGAAGCTAAAAGATTGACTA12.941176470588235No Hit
TTATAATTCCAATCACTCGTTTAAAAGCGAAAGAAAAAACAATTACACCGA12.941176470588235No Hit
GTTGTGCATCATCAACAACTCGAGCTCACTTGTGTTGTGAACAACGAATTT12.941176470588235No Hit
TCCCCAGAGATCATAGGCTGCAATCGTAGTGCTTGGTTTTGTGGCTCTAAA12.941176470588235No Hit
CACCTTTGGTATAATCAATAGCGCATCAGCAAATTCAGCGATGACCCTGTC12.941176470588235No Hit
GTCCAAGAGGGACGTTTCAGAAAACAACAACAACATGCGAGGTCAAAATCT12.941176470588235No Hit
TATCAACGCAGAGTACGGGGGGTTCGAGTGTGAGCATGCCTGTCGGGACCC12.941176470588235No Hit
GATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG12.941176470588235No Hit
GTATAGTATAGGAACAAGCCTAACTTGACATTGACATGGCAAGTAACTAAG12.941176470588235No Hit
AGTCACGTGTTCAACCTCAACGGTGGCCATTTCGAAGGAAAATATAGCTGT12.941176470588235No Hit
CAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCT12.941176470588235No Hit
TCACATTGCTTTAATTCCTGAACTTGTACTTAAGCAGTTCCAGATCTGGAG12.941176470588235No Hit
TTTCTATTCTGTTAACGTCCTTCAAGATATTTACCATTCATATGTATGGTC12.941176470588235No Hit
CATTCAACTTGACCAGCACTTGTATCATGTTTAGATAAGCTACATTTGAGG12.941176470588235No Hit
GTAATAAAGTCGTTGTCTGGATTCAGAAGGAAATGAGTTGTGTATGTGTTT12.941176470588235No Hit
TAGTAGATCAATCCCAATAACCTGATTCACTTGGTCAAGTTTGCTGATAAT12.941176470588235No Hit
ACTTGGAAGCCTTTCAATCCTATTCTTGGGGAGACATATGAGATGGTCAAC12.941176470588235No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTTAGAAGTTCGAAGGCGATCAGA12.941176470588235No Hit
CGTACATCTGTAACGGTTACAATGGTATTGTTGAAACTTGCTTGAACATGA12.941176470588235No Hit
GGTTAGATGGATCATATCAGCTCACGAGTTCGTTTTCACCTATGTTTTAGC12.941176470588235No Hit
AAACAAAACCATCTCAAAGTTTCTTGAGAAGAAAAAAAGGGTCAAGAAAGA12.941176470588235No Hit
CAAAAACATAGAACTTGAATTCAACAATAAACTGCAACATGAATGTATTGT12.941176470588235No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)