FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_86.3410000000ab63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_86.3410000000ab63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGTCTCTGAAATCGTGATGTCAAAAAGGAATGATATGCGATTATTAAC12.4390243902439024No Hit
CCTCCAGATTTAACATGACCCTCACTGGATTTACCGGGTGATCCCGGTTTG12.4390243902439024No Hit
CTTGAGCCCCGCTACATTGTCGGCGCGGAATCACTTGACCAGTGAGCTATT12.4390243902439024No Hit
GAGAACTGGTCAGCATCTTGCTTCTGGAAACCAGTTCCTCAGCCAAAGAAA12.4390243902439024No Hit
ATATAATATTGATATTTGAGCCTTTCTTTTACTTTTGAAGGATGGAGGGGG12.4390243902439024No Hit
GGACAAAAGGAGCCATCCATATGACATCACCAGCCTGAACTGGATACCAGT12.4390243902439024No Hit
GGCAAGTACCTTCGTGCAATAGATCGAAAGCCTTATTGATTTCTCCCAAGG12.4390243902439024No Hit
TGTCTAGATAGGTGTTTCTTTAATCCTTTATCTATGCCCTTGTATGATCCA12.4390243902439024No Hit
AGCTTACTCACTCTTCCTCATCACCGTATGGTTTCTCTTATTTTTCCAAAA12.4390243902439024No Hit
CTCCGGTACACTCTCTGACGATTTGTTCAACAACTGTTCCTCTCTTAGATA12.4390243902439024No Hit
GACCGGGAACGGCTCTCTCGGGAGCTTTCCCCGGGCGTCGAACAGTCAGCT12.4390243902439024No Hit
GAGACAAACCAGTAACCAAAATTTTTGAATCTCCTCCAAACAACAAGTCTA12.4390243902439024No Hit
CGTTGAGCGAGAGCCCTTCCACACGGGACTCCCGGATCACTATGGCCGACT12.4390243902439024No Hit
TGTCTCATGTGGATACTGGTTCTTCCCATTGTAATCTTTTGGTTACATACT12.4390243902439024No Hit
ATTGTGGAGTGTGTTACGCTGATGTTATCTGGAGTAGAAACCAACATGGAG12.4390243902439024No Hit
GGCATTTATATTGACCCTCTTGCCTAAGCTACCTGATAACTATGATTAACT12.4390243902439024No Hit
ATTCTCAACTGGTCCTTTGTCAGGAACGACCAGCCCAGGCACGAAACCTGT12.4390243902439024No Hit
ATATAGTCTTGCTGCTGTGACAAAATGATATATTCACGAAAGAGGATGGTG12.4390243902439024No Hit
ATTCTTGGAGGTTATGATAGAGATGGACCTCAATTGTACATGATTGAACCA12.4390243902439024No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAG12.4390243902439024No Hit
GTCATAGGGTTCTTTTCACCTTTCCCTCACGGTACTTGTACGCCTGTCTCT12.4390243902439024No Hit
GATCAATTAACAAGGATTATTCTAGAAACAGTGTGGACAAAATCTGTCTCT12.4390243902439024No Hit
TAACCACAGGTGTCTTGAAAGTGTCGAGAAACTGGAGAGTGGCTCTTTTTG12.4390243902439024No Hit
CACCATTGCAGATGCTTAAGTCAACTATTTTAATAAATTGATGACCAATTG12.4390243902439024No Hit
ACGTAGGTTTGAGTTATTTTGTAACAACCAAATGAAGAAAATGGAAAGACC12.4390243902439024No Hit
GATAGCACCTTTTCTTGGCAGTTTGAATCTGTCGCTTCGCATCTTTCTGCT12.4390243902439024No Hit
GTATCTCATCTTCCTTTCTTTTGACCTCGCAGCAGACTGCCTGTTTGCCCA12.4390243902439024No Hit
TCCTTGCTCTCTGTATCTTTTGCATTTGTCATCTTCATGGCACTCATTTCT12.4390243902439024No Hit
GAACTACTCACACATTATTATGGAGAAACTCGAGCTTGTCGATCGACTCTA12.4390243902439024No Hit
CTGCTGGACAAGAAAGATTTCAAAGTTTGGGAGTTGCGTTTTACCGAGGAG12.4390243902439024No Hit
GTACATCCAAGAACCTTTGTATTTACAATGAAAAAAATGGGGTTGTCAAGG12.4390243902439024No Hit
GGACTGAATCCAACGCCACCGTTAAAGAACATCTCACTAAACGGCCGTGGA12.4390243902439024No Hit
GTTCAGGACATTCTTCCTTGAGCGTAGTGTCCGTCCACCTGTGCTTCTCTC12.4390243902439024No Hit
GCTCTGACCAGGCCTCCTTCTATCGAGATCGAATTGCAAATCAGATTCAGT12.4390243902439024No Hit
GTGATACTTGCTGCATGTTAGGTGGTTTAAAAAACCATTATCTTCATGCTG12.4390243902439024No Hit
TTCCTCTTGTTTGGTCCGAGAATGGAGCTTGGTTGGAAGAGCAATGGAGTT12.4390243902439024No Hit
CAATAATCTGAACCTTCTTCCACTGTAGCTCCCAGTTTTCTAAGCTCTGTG12.4390243902439024No Hit
GTACCCATATGTTACTGGAACTTCAATTGTTGCAATCAAGTACAAAGATGG12.4390243902439024No Hit
GATACGTTCCTCGTGCTGTTCTTATGGATCTTGAGCCTGGTACTATGGATA12.4390243902439024No Hit
GTCTCGATCATCAAGAACGCGCTTACGCGCCCGGCGCGCCAGATTGTCGAA12.4390243902439024No Hit
TGTTATCTCTATATACGAATGCTGTGATTCATCTGTCTCTTATACACATCT12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)