##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_86.3410000000ab63.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.853658536585364 0.0 0.0 0.0 0.0 0.0 2 37.5609756097561 0.0 0.0 0.0 0.0 0.0 3 37.853658536585364 0.0 0.0 0.0 0.0 0.0 4 37.707317073170735 0.0 0.0 0.0 0.0 0.0 5 37.707317073170735 0.0 0.0 0.0 0.0 0.0 6 39.5609756097561 0.0 0.0 0.0 0.0 0.0 7 39.90243902439025 0.0 0.0 0.0 0.0 0.0 8 39.609756097560975 0.0 0.0 0.0 0.0 0.0 9 39.75609756097561 0.0 0.0 0.0 0.0 0.0 10 39.707317073170735 0.0 0.0 0.0 0.0 0.0 11 39.75609756097561 0.0 0.0 0.0 0.0 0.0 12 39.75609756097561 0.0 0.0 0.0 0.0 0.0 13 39.80487804878049 0.0 0.0 0.0 0.0 0.0 14 39.80487804878049 0.0 0.0 0.0 0.0 0.0 15 39.80487804878049 0.0 0.0 0.0 0.0 0.0 16 39.80487804878049 0.0 0.0 0.0 0.0 0.0 17 39.853658536585364 0.0 0.0 0.0 0.0 0.0 18 39.80487804878049 0.0 0.0 0.0 0.0 0.0 19 39.58536585365854 0.0 0.0 0.0 0.0 0.0 20 39.48780487804878 0.0 0.0 0.0 0.0 0.0 21 39.4390243902439 0.0 0.0 0.0 0.0 0.0 22 39.48780487804878 0.0 0.0 0.0 0.0 0.0 23 39.609756097560975 0.0 0.0 0.0 0.0 0.0 24 39.609756097560975 0.0 0.0 0.0 0.0 0.0 25 39.90243902439025 0.0 0.0 0.0 0.0 0.0 26 39.853658536585364 0.0 0.0 0.0 0.0 0.0 27 39.707317073170735 0.0 0.0 0.0 0.0 0.0 28 39.75609756097561 0.0 0.0 0.0 0.0 0.0 29 39.609756097560975 0.0 0.0 0.0 0.0 0.0 30 39.5609756097561 0.0 0.0 0.0 0.0 0.0 31 39.75609756097561 0.0 0.0 0.0 0.0 0.0 32 39.65853658536585 0.0 0.0 0.0 0.0 0.0 33 39.609756097560975 0.0 0.0 0.0 0.0 0.0 34 39.75609756097561 0.0 0.0 0.0 0.0 0.0 35 39.75609756097561 0.0 0.0 0.0 0.0 0.0 36 39.46341463414634 0.0 0.0 0.0 0.0 0.0 37 39.5609756097561 0.0 0.0 0.0 0.0 0.0 38 39.707317073170735 0.0 0.0 0.0 0.0 0.0 39 39.65853658536585 0.0 0.0 0.0 0.0 0.0 40 39.707317073170735 0.0 0.0 0.0 0.0 0.0 41 39.5609756097561 0.0 0.0 0.0 0.0 0.0 42 39.34146341463415 0.0 0.0 0.0 0.0 0.0 43 39.19512195121951 0.0 0.0 0.0 0.0 0.0 44 39.51219512195122 0.0 0.0 0.0 0.0 0.0 45 39.609756097560975 0.0 0.0 0.0 0.0 0.0 46 39.65853658536585 0.0 0.0 0.0 0.0 0.0 47 39.75609756097561 0.0 0.0 0.0 0.0 0.0 48 39.80487804878049 0.0 0.0 0.0 0.0 0.0 49 39.853658536585364 0.0 0.0 0.0 0.0 0.0 50 39.51219512195122 0.0 0.0 0.0 0.0 0.0 51 39.46341463414634 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2116 1 0.0 2116 2 0.0 2116 3 0.0 2116 4 0.0 2116 5 0.0 2116 6 0.0 2116 7 0.0 2116 8 0.5 2116 9 0.0 2116 10 0.0 2116 11 0.0 2116 12 -1.5 2116 13 -1.5 2116 14 0.0 2116 15 -1.5 2116 16 0.0 2116 17 0.0 2116 18 0.0 2116 19 0.5 2116 20 0.0 2116 21 0.5 2116 22 0.5 2116 23 1.0 2116 24 0.5 2116 25 0.5 2116 26 0.0 2116 27 0.5 2116 28 1.0 2116 29 0.5 2116 30 0.5 2116 31 0.5 2116 32 0.5 2116 33 1.0 2116 34 1.0 2116 35 0.0 2116 36 0.5 2116 37 1.0 2116 38 0.5 2116 39 1.0 2116 40 1.0 2116 41 0.5 2116 42 1.0 2116 43 0.0 2116 44 0.0 2116 45 0.0 2116 46 0.0 2116 47 0.0 2116 48 0.0 2116 49 0.0 2116 50 0.5 2116 51 0.5 1201 1 0.0 1201 2 0.0 1201 3 0.0 1201 4 0.0 1201 5 0.0 1201 6 0.0 1201 7 0.0 1201 8 0.5 1201 9 0.0 1201 10 0.0 1201 11 0.0 1201 12 0.5 1201 13 0.5 1201 14 0.0 1201 15 0.5 1201 16 0.0 1201 17 0.0 1201 18 0.0 1201 19 -1.5 1201 20 0.0 1201 21 -1.5 1201 22 -1.5 1201 23 -1.0 1201 24 -1.5 1201 25 -1.5 1201 26 0.0 1201 27 -1.5 1201 28 -1.0 1201 29 -1.5 1201 30 -1.5 1201 31 0.5 1201 32 -1.5 1201 33 -1.0 1201 34 -1.0 1201 35 0.0 1201 36 -1.5 1201 37 -1.0 1201 38 -1.5 1201 39 -1.0 1201 40 -1.0 1201 41 -1.5 1201 42 -1.0 1201 43 0.0 1201 44 0.0 1201 45 0.0 1201 46 0.0 1201 47 0.0 1201 48 0.0 1201 49 0.0 1201 50 -1.5 1201 51 -1.5 2101 1 0.0 2101 2 0.0 2101 3 0.0 2101 4 0.0 2101 5 0.0 2101 6 0.0 2101 7 0.0 2101 8 -1.5 2101 9 0.0 2101 10 0.0 2101 11 0.0 2101 12 0.5 2101 13 0.5 2101 14 0.0 2101 15 0.5 2101 16 0.0 2101 17 0.0 2101 18 0.0 2101 19 0.5 2101 20 0.0 2101 21 0.5 2101 22 0.5 2101 23 -1.0 2101 24 0.5 2101 25 0.5 2101 26 0.0 2101 27 0.5 2101 28 1.0 2101 29 0.5 2101 30 0.5 2101 31 0.5 2101 32 0.5 2101 33 1.0 2101 34 1.0 2101 35 0.0 2101 36 0.5 2101 37 -1.0 2101 38 0.5 2101 39 1.0 2101 40 -1.0 2101 41 0.5 2101 42 1.0 2101 43 0.0 2101 44 0.0 2101 45 0.0 2101 46 0.0 2101 47 0.0 2101 48 0.0 2101 49 0.0 2101 50 0.5 2101 51 0.5 2212 1 0.0 2212 2 0.0 2212 3 0.0 2212 4 0.0 2212 5 0.0 2212 6 0.0 2212 7 0.0 2212 8 0.5 2212 9 0.0 2212 10 0.0 2212 11 0.0 2212 12 0.5 2212 13 0.5 2212 14 0.0 2212 15 0.5 2212 16 0.0 2212 17 0.0 2212 18 0.0 2212 19 0.5 2212 20 0.0 2212 21 0.5 2212 22 0.5 2212 23 1.0 2212 24 0.5 2212 25 0.5 2212 26 0.0 2212 27 0.5 2212 28 -1.0 2212 29 0.5 2212 30 0.5 2212 31 -1.5 2212 32 0.5 2212 33 -1.0 2212 34 -1.0 2212 35 0.0 2212 36 0.5 2212 37 1.0 2212 38 0.5 2212 39 -1.0 2212 40 1.0 2212 41 0.5 2212 42 -1.0 2212 43 0.0 2212 44 0.0 2212 45 0.0 2212 46 0.0 2212 47 0.0 2212 48 0.0 2212 49 0.0 2212 50 0.5 2212 51 0.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 38 4.0 39 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.21951219512195 17.073170731707318 14.634146341463413 17.073170731707318 2 21.951219512195124 26.82926829268293 41.46341463414634 9.75609756097561 3 12.195121951219512 26.82926829268293 36.58536585365854 24.390243902439025 4 4.878048780487805 21.951219512195124 26.82926829268293 46.34146341463415 5 12.195121951219512 31.70731707317073 39.02439024390244 17.073170731707318 6 34.146341463414636 31.70731707317073 17.073170731707318 17.073170731707318 7 24.390243902439025 21.951219512195124 26.82926829268293 26.82926829268293 8 9.75609756097561 39.02439024390244 31.70731707317073 19.51219512195122 9 19.51219512195122 12.195121951219512 26.82926829268293 41.46341463414634 10 12.195121951219512 34.146341463414636 36.58536585365854 17.073170731707318 11 41.46341463414634 12.195121951219512 26.82926829268293 19.51219512195122 12 24.390243902439025 29.268292682926827 34.146341463414636 12.195121951219512 13 24.390243902439025 21.951219512195124 29.268292682926827 24.390243902439025 14 14.634146341463413 24.390243902439025 29.268292682926827 31.70731707317073 15 9.75609756097561 24.390243902439025 41.46341463414634 24.390243902439025 16 7.317073170731707 14.634146341463413 48.78048780487805 29.268292682926827 17 21.951219512195124 9.75609756097561 29.268292682926827 39.02439024390244 18 17.073170731707318 21.951219512195124 51.21951219512195 9.75609756097561 19 17.073170731707318 26.82926829268293 31.70731707317073 24.390243902439025 20 21.951219512195124 21.951219512195124 39.02439024390244 17.073170731707318 21 21.951219512195124 21.951219512195124 31.70731707317073 24.390243902439025 22 24.390243902439025 19.51219512195122 36.58536585365854 19.51219512195122 23 14.634146341463413 24.390243902439025 46.34146341463415 14.634146341463413 24 17.073170731707318 29.268292682926827 29.268292682926827 24.390243902439025 25 31.70731707317073 17.073170731707318 21.951219512195124 29.268292682926827 26 19.51219512195122 31.70731707317073 29.268292682926827 19.51219512195122 27 29.268292682926827 34.146341463414636 31.70731707317073 4.878048780487805 28 19.51219512195122 34.146341463414636 26.82926829268293 19.51219512195122 29 14.634146341463413 31.70731707317073 29.268292682926827 24.390243902439025 30 12.195121951219512 21.951219512195124 39.02439024390244 26.82926829268293 31 24.390243902439025 24.390243902439025 17.073170731707318 34.146341463414636 32 14.634146341463413 31.70731707317073 24.390243902439025 29.268292682926827 33 19.51219512195122 34.146341463414636 21.951219512195124 24.390243902439025 34 24.390243902439025 21.951219512195124 39.02439024390244 14.634146341463413 35 26.82926829268293 12.195121951219512 31.70731707317073 29.268292682926827 36 21.951219512195124 26.82926829268293 31.70731707317073 19.51219512195122 37 19.51219512195122 21.951219512195124 26.82926829268293 31.70731707317073 38 19.51219512195122 31.70731707317073 34.146341463414636 14.634146341463413 39 19.51219512195122 31.70731707317073 29.268292682926827 19.51219512195122 40 24.390243902439025 29.268292682926827 17.073170731707318 29.268292682926827 41 19.51219512195122 29.268292682926827 36.58536585365854 14.634146341463413 42 29.268292682926827 31.70731707317073 21.951219512195124 17.073170731707318 43 17.073170731707318 24.390243902439025 34.146341463414636 24.390243902439025 44 21.951219512195124 21.951219512195124 31.70731707317073 24.390243902439025 45 14.634146341463413 26.82926829268293 31.70731707317073 26.82926829268293 46 21.951219512195124 36.58536585365854 26.82926829268293 14.634146341463413 47 19.51219512195122 19.51219512195122 29.268292682926827 31.70731707317073 48 24.390243902439025 24.390243902439025 34.146341463414636 17.073170731707318 49 26.82926829268293 34.146341463414636 21.951219512195124 17.073170731707318 50 19.51219512195122 17.073170731707318 31.70731707317073 31.70731707317073 51 31.70731707317073 26.82926829268293 34.146341463414636 7.317073170731707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.5 31 1.0 32 3.0 33 5.0 34 4.0 35 3.0 36 5.0 37 7.0 38 4.0 39 1.0 40 1.5 41 2.0 42 2.5 43 3.0 44 4.0 45 5.0 46 4.0 47 3.0 48 3.0 49 3.0 50 2.5 51 2.0 52 2.0 53 2.0 54 1.5 55 1.0 56 0.5 57 0.0 58 0.0 59 0.0 60 0.5 61 1.0 62 1.0 63 1.0 64 0.5 65 0.0 66 0.5 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 41.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGTCTCTGAAATCGTGATGTCAAAAAGGAATGATATGCGATTATTAAC 1 2.4390243902439024 No Hit CCTCCAGATTTAACATGACCCTCACTGGATTTACCGGGTGATCCCGGTTTG 1 2.4390243902439024 No Hit CTTGAGCCCCGCTACATTGTCGGCGCGGAATCACTTGACCAGTGAGCTATT 1 2.4390243902439024 No Hit GAGAACTGGTCAGCATCTTGCTTCTGGAAACCAGTTCCTCAGCCAAAGAAA 1 2.4390243902439024 No Hit ATATAATATTGATATTTGAGCCTTTCTTTTACTTTTGAAGGATGGAGGGGG 1 2.4390243902439024 No Hit GGACAAAAGGAGCCATCCATATGACATCACCAGCCTGAACTGGATACCAGT 1 2.4390243902439024 No Hit GGCAAGTACCTTCGTGCAATAGATCGAAAGCCTTATTGATTTCTCCCAAGG 1 2.4390243902439024 No Hit TGTCTAGATAGGTGTTTCTTTAATCCTTTATCTATGCCCTTGTATGATCCA 1 2.4390243902439024 No Hit AGCTTACTCACTCTTCCTCATCACCGTATGGTTTCTCTTATTTTTCCAAAA 1 2.4390243902439024 No Hit CTCCGGTACACTCTCTGACGATTTGTTCAACAACTGTTCCTCTCTTAGATA 1 2.4390243902439024 No Hit GACCGGGAACGGCTCTCTCGGGAGCTTTCCCCGGGCGTCGAACAGTCAGCT 1 2.4390243902439024 No Hit GAGACAAACCAGTAACCAAAATTTTTGAATCTCCTCCAAACAACAAGTCTA 1 2.4390243902439024 No Hit CGTTGAGCGAGAGCCCTTCCACACGGGACTCCCGGATCACTATGGCCGACT 1 2.4390243902439024 No Hit TGTCTCATGTGGATACTGGTTCTTCCCATTGTAATCTTTTGGTTACATACT 1 2.4390243902439024 No Hit ATTGTGGAGTGTGTTACGCTGATGTTATCTGGAGTAGAAACCAACATGGAG 1 2.4390243902439024 No Hit GGCATTTATATTGACCCTCTTGCCTAAGCTACCTGATAACTATGATTAACT 1 2.4390243902439024 No Hit ATTCTCAACTGGTCCTTTGTCAGGAACGACCAGCCCAGGCACGAAACCTGT 1 2.4390243902439024 No Hit ATATAGTCTTGCTGCTGTGACAAAATGATATATTCACGAAAGAGGATGGTG 1 2.4390243902439024 No Hit ATTCTTGGAGGTTATGATAGAGATGGACCTCAATTGTACATGATTGAACCA 1 2.4390243902439024 No Hit GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAG 1 2.4390243902439024 No Hit GTCATAGGGTTCTTTTCACCTTTCCCTCACGGTACTTGTACGCCTGTCTCT 1 2.4390243902439024 No Hit GATCAATTAACAAGGATTATTCTAGAAACAGTGTGGACAAAATCTGTCTCT 1 2.4390243902439024 No Hit TAACCACAGGTGTCTTGAAAGTGTCGAGAAACTGGAGAGTGGCTCTTTTTG 1 2.4390243902439024 No Hit CACCATTGCAGATGCTTAAGTCAACTATTTTAATAAATTGATGACCAATTG 1 2.4390243902439024 No Hit ACGTAGGTTTGAGTTATTTTGTAACAACCAAATGAAGAAAATGGAAAGACC 1 2.4390243902439024 No Hit GATAGCACCTTTTCTTGGCAGTTTGAATCTGTCGCTTCGCATCTTTCTGCT 1 2.4390243902439024 No Hit GTATCTCATCTTCCTTTCTTTTGACCTCGCAGCAGACTGCCTGTTTGCCCA 1 2.4390243902439024 No Hit TCCTTGCTCTCTGTATCTTTTGCATTTGTCATCTTCATGGCACTCATTTCT 1 2.4390243902439024 No Hit GAACTACTCACACATTATTATGGAGAAACTCGAGCTTGTCGATCGACTCTA 1 2.4390243902439024 No Hit CTGCTGGACAAGAAAGATTTCAAAGTTTGGGAGTTGCGTTTTACCGAGGAG 1 2.4390243902439024 No Hit GTACATCCAAGAACCTTTGTATTTACAATGAAAAAAATGGGGTTGTCAAGG 1 2.4390243902439024 No Hit GGACTGAATCCAACGCCACCGTTAAAGAACATCTCACTAAACGGCCGTGGA 1 2.4390243902439024 No Hit GTTCAGGACATTCTTCCTTGAGCGTAGTGTCCGTCCACCTGTGCTTCTCTC 1 2.4390243902439024 No Hit GCTCTGACCAGGCCTCCTTCTATCGAGATCGAATTGCAAATCAGATTCAGT 1 2.4390243902439024 No Hit GTGATACTTGCTGCATGTTAGGTGGTTTAAAAAACCATTATCTTCATGCTG 1 2.4390243902439024 No Hit TTCCTCTTGTTTGGTCCGAGAATGGAGCTTGGTTGGAAGAGCAATGGAGTT 1 2.4390243902439024 No Hit CAATAATCTGAACCTTCTTCCACTGTAGCTCCCAGTTTTCTAAGCTCTGTG 1 2.4390243902439024 No Hit GTACCCATATGTTACTGGAACTTCAATTGTTGCAATCAAGTACAAAGATGG 1 2.4390243902439024 No Hit GATACGTTCCTCGTGCTGTTCTTATGGATCTTGAGCCTGGTACTATGGATA 1 2.4390243902439024 No Hit GTCTCGATCATCAAGAACGCGCTTACGCGCCCGGCGCGCCAGATTGTCGAA 1 2.4390243902439024 No Hit TGTTATCTCTATATACGAATGCTGTGATTCATCTGTCTCTTATACACATCT 1 2.4390243902439024 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 2.4390243902439024 34 0.0 0.0 2.4390243902439024 35 0.0 0.0 2.4390243902439024 36 0.0 0.0 2.4390243902439024 37 0.0 0.0 2.4390243902439024 38 0.0 0.0 2.4390243902439024 39 0.0 0.0 2.4390243902439024 >>END_MODULE >>Kmer Content pass >>END_MODULE