##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_85.3410000000ab4a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.0 0.0 0.0 0.0 0.0 0.0 2 38.0 0.0 0.0 0.0 0.0 0.0 3 38.0 0.0 0.0 0.0 0.0 0.0 4 38.0 0.0 0.0 0.0 0.0 0.0 5 37.45454545454545 0.0 0.0 0.0 0.0 0.0 6 40.0 0.0 0.0 0.0 0.0 0.0 7 39.81818181818182 0.0 0.0 0.0 0.0 0.0 8 39.81818181818182 0.0 0.0 0.0 0.0 0.0 9 39.81818181818182 0.0 0.0 0.0 0.0 0.0 10 40.0 0.0 0.0 0.0 0.0 0.0 11 39.81818181818182 0.0 0.0 0.0 0.0 0.0 12 39.63636363636363 0.0 0.0 0.0 0.0 0.0 13 40.0 0.0 0.0 0.0 0.0 0.0 14 39.81818181818182 0.0 0.0 0.0 0.0 0.0 15 40.0 0.0 0.0 0.0 0.0 0.0 16 40.0 0.0 0.0 0.0 0.0 0.0 17 39.63636363636363 0.0 0.0 0.0 0.0 0.0 18 40.0 0.0 0.0 0.0 0.0 0.0 19 39.81818181818182 0.0 0.0 0.0 0.0 0.0 20 39.63636363636363 0.0 0.0 0.0 0.0 0.0 21 39.81818181818182 0.0 0.0 0.0 0.0 0.0 22 40.0 0.0 0.0 0.0 0.0 0.0 23 39.81818181818182 0.0 0.0 0.0 0.0 0.0 24 39.81818181818182 0.0 0.0 0.0 0.0 0.0 25 39.81818181818182 0.0 0.0 0.0 0.0 0.0 26 39.81818181818182 0.0 0.0 0.0 0.0 0.0 27 40.0 0.0 0.0 0.0 0.0 0.0 28 39.81818181818182 0.0 0.0 0.0 0.0 0.0 29 39.81818181818182 0.0 0.0 0.0 0.0 0.0 30 40.0 0.0 0.0 0.0 0.0 0.0 31 40.0 0.0 0.0 0.0 0.0 0.0 32 39.63636363636363 0.0 0.0 0.0 0.0 0.0 33 39.81818181818182 0.0 0.0 0.0 0.0 0.0 34 40.0 0.0 0.0 0.0 0.0 0.0 35 40.0 0.0 0.0 0.0 0.0 0.0 36 40.0 0.0 0.0 0.0 0.0 0.0 37 39.81818181818182 0.0 0.0 0.0 0.0 0.0 38 40.0 0.0 0.0 0.0 0.0 0.0 39 39.81818181818182 0.0 0.0 0.0 0.0 0.0 40 39.81818181818182 0.0 0.0 0.0 0.0 0.0 41 39.63636363636363 0.0 0.0 0.0 0.0 0.0 42 40.0 0.0 0.0 0.0 0.0 0.0 43 40.0 0.0 0.0 0.0 0.0 0.0 44 39.63636363636363 0.0 0.0 0.0 0.0 0.0 45 40.0 0.0 0.0 0.0 0.0 0.0 46 39.63636363636363 0.0 0.0 0.0 0.0 0.0 47 39.09090909090909 0.0 0.0 0.0 0.0 0.0 48 39.63636363636363 0.0 0.0 0.0 0.0 0.0 49 39.27272727272727 0.0 0.0 0.0 0.0 0.0 50 38.81818181818182 0.0 0.0 0.0 0.0 0.0 51 36.63636363636363 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2211 1 0.0 2211 2 0.0 2211 3 0.0 2211 4 0.0 2211 5 0.0 2211 6 0.0 2211 7 0.0 2211 8 0.0 2211 9 0.0 2211 10 0.0 2211 11 0.0 2211 12 0.0 2211 13 0.0 2211 14 0.0 2211 15 0.0 2211 16 0.0 2211 17 0.0 2211 18 0.0 2211 19 0.0 2211 20 0.0 2211 21 0.0 2211 22 0.0 2211 23 0.0 2211 24 0.0 2211 25 0.0 2211 26 0.0 2211 27 0.0 2211 28 0.0 2211 29 0.0 2211 30 0.0 2211 31 0.0 2211 32 0.0 2211 33 0.0 2211 34 0.0 2211 35 0.0 2211 36 0.0 2211 37 0.0 2211 38 0.0 2211 39 0.0 2211 40 0.0 2211 41 0.0 2211 42 0.0 2211 43 0.0 2211 44 0.0 2211 45 0.0 2211 46 0.0 2211 47 0.0 2211 48 0.0 2211 49 0.0 2211 50 0.0 2211 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 38 2.0 39 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.54545454545454 18.181818181818183 9.090909090909092 18.181818181818183 2 0.0 27.27272727272727 36.36363636363637 36.36363636363637 3 36.36363636363637 36.36363636363637 18.181818181818183 9.090909090909092 4 9.090909090909092 27.27272727272727 54.54545454545454 9.090909090909092 5 9.090909090909092 45.45454545454545 27.27272727272727 18.181818181818183 6 9.090909090909092 54.54545454545454 18.181818181818183 18.181818181818183 7 27.27272727272727 45.45454545454545 9.090909090909092 18.181818181818183 8 18.181818181818183 27.27272727272727 9.090909090909092 45.45454545454545 9 18.181818181818183 9.090909090909092 36.36363636363637 36.36363636363637 10 36.36363636363637 27.27272727272727 27.27272727272727 9.090909090909092 11 54.54545454545454 18.181818181818183 18.181818181818183 9.090909090909092 12 27.27272727272727 36.36363636363637 0.0 36.36363636363637 13 9.090909090909092 18.181818181818183 63.63636363636363 9.090909090909092 14 9.090909090909092 9.090909090909092 45.45454545454545 36.36363636363637 15 0.0 27.27272727272727 36.36363636363637 36.36363636363637 16 9.090909090909092 9.090909090909092 36.36363636363637 45.45454545454545 17 36.36363636363637 9.090909090909092 45.45454545454545 9.090909090909092 18 18.181818181818183 27.27272727272727 36.36363636363637 18.181818181818183 19 18.181818181818183 9.090909090909092 54.54545454545454 18.181818181818183 20 36.36363636363637 27.27272727272727 18.181818181818183 18.181818181818183 21 9.090909090909092 27.27272727272727 27.27272727272727 36.36363636363637 22 9.090909090909092 45.45454545454545 18.181818181818183 27.27272727272727 23 18.181818181818183 36.36363636363637 36.36363636363637 9.090909090909092 24 18.181818181818183 27.27272727272727 18.181818181818183 36.36363636363637 25 27.27272727272727 45.45454545454545 9.090909090909092 18.181818181818183 26 27.27272727272727 27.27272727272727 9.090909090909092 36.36363636363637 27 9.090909090909092 36.36363636363637 27.27272727272727 27.27272727272727 28 9.090909090909092 45.45454545454545 18.181818181818183 27.27272727272727 29 27.27272727272727 45.45454545454545 18.181818181818183 9.090909090909092 30 27.27272727272727 36.36363636363637 27.27272727272727 9.090909090909092 31 27.27272727272727 45.45454545454545 18.181818181818183 9.090909090909092 32 27.27272727272727 27.27272727272727 27.27272727272727 18.181818181818183 33 18.181818181818183 36.36363636363637 27.27272727272727 18.181818181818183 34 18.181818181818183 45.45454545454545 9.090909090909092 27.27272727272727 35 0.0 36.36363636363637 27.27272727272727 36.36363636363637 36 18.181818181818183 54.54545454545454 9.090909090909092 18.181818181818183 37 54.54545454545454 9.090909090909092 27.27272727272727 9.090909090909092 38 9.090909090909092 36.36363636363637 45.45454545454545 9.090909090909092 39 0.0 54.54545454545454 0.0 45.45454545454545 40 27.27272727272727 27.27272727272727 18.181818181818183 27.27272727272727 41 36.36363636363637 27.27272727272727 9.090909090909092 27.27272727272727 42 0.0 72.72727272727273 18.181818181818183 9.090909090909092 43 27.27272727272727 27.27272727272727 27.27272727272727 18.181818181818183 44 0.0 27.27272727272727 36.36363636363637 36.36363636363637 45 0.0 36.36363636363637 36.36363636363637 27.27272727272727 46 36.36363636363637 36.36363636363637 18.181818181818183 9.090909090909092 47 36.36363636363637 36.36363636363637 9.090909090909092 18.181818181818183 48 9.090909090909092 54.54545454545454 18.181818181818183 18.181818181818183 49 18.181818181818183 45.45454545454545 18.181818181818183 18.181818181818183 50 27.27272727272727 27.27272727272727 27.27272727272727 18.181818181818183 51 9.090909090909092 45.45454545454545 27.27272727272727 18.181818181818183 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.0 25 0.5 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.5 33 1.0 34 1.0 35 1.0 36 1.0 37 1.0 38 1.0 39 1.0 40 0.5 41 0.0 42 1.0 43 2.0 44 1.0 45 0.0 46 0.0 47 0.0 48 1.0 49 2.0 50 1.0 51 0.0 52 0.5 53 1.0 54 0.5 55 0.0 56 0.5 57 1.0 58 0.5 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 11.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCAAAAAACATCTGTTTATTATTCAGCAGAGTCACATAAAGACTCTGAAGT 1 9.090909090909092 No Hit GAATCAAATGGAATCCTCGAATAGAATCAGATGGAATCATCAAATGGAATC 1 9.090909090909092 No Hit ATGGAAACTGGCTTTTTCTCGAAAGCACTGAAGATCGTAGGTGCAACAACA 1 9.090909090909092 No Hit CCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAA 1 9.090909090909092 No Hit TCCCTAACAAGCTCACTTTCTCGGCCAATGTCAACCGTCCGATCAAATCTC 1 9.090909090909092 No Hit GCGTGTGGCTCAACTCGTTGTCTTGGCTGAAGACTGCAACCAGCCTGATTA 1 9.090909090909092 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1 9.090909090909092 No Hit CTTAATGATTAATTAACTAGCCATGGATCTGCAACAGACCCAGTTAAGGAT 1 9.090909090909092 No Hit GAAATCACGATGTCCTGGGGCATCAATGACTGTGCAGTAGTACTTGGTGGT 1 9.090909090909092 No Hit GTATCGTCTCAGGTCTTATATGGATTCAGAAATCATGGCGATTATGTCTCA 1 9.090909090909092 No Hit ATTTTACTCGGACCAGGTCAAACCACTAATGTTTTAGTCAAAACCGACCGG 1 9.090909090909092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE