FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_84.3410000000ab3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_84.3410000000ab3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGTGATGCGTCGTCTCAAGAGCATTGCTTCGGGTCGGACCTCCAT12.857142857142857No Hit
ATAGAGAGGAGCCCGAGGATGAGGCATCATCGGGTGAACAACAATCTTATG12.857142857142857No Hit
AGACGCTGCTGGTCCGGAGGGATACCTTCCTTGTCCTGGATCTTAGCCTTC12.857142857142857No Hit
TGTGAGCCGTAAGAATGGTGACAACTCTTTGAATGGATCCTCCCTTATTGA12.857142857142857No Hit
ACCCTCTCCTGGGCCCTGAGTGTCAGAAAATGTTCGAACAAGGACAAGTTG12.857142857142857No Hit
ATATTGAACGATGCCAATTTTCACAGCATGATAAAACTGAGGACCAAGACT12.857142857142857No Hit
AAATTAGACCCGTTAACATTCTTCACTGGTGCAAACTCCTCTTCCCTTAAA12.857142857142857No Hit
GAAATATACCAAAGAGCAAAACTCACAAGTGAGCATGTCTGTCTCTTATAC12.857142857142857No Hit
GCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGAGTGGGCAGGCTGTT12.857142857142857No Hit
AAGAAAGGTATAACACAGGAACGGAAACATAGTTGAACAATTATTCATCAG12.857142857142857No Hit
GTCCTGAAGTCCTCCTCCTTGATTACCCAAGCCATCAAAGGCGACTGCTAC12.857142857142857No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAG12.857142857142857No Hit
CTCATGCCATCTCACATCGCAGCAACCGCACTTAACTTAATCGGTGCACCA12.857142857142857No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC12.857142857142857Illumina PCR Primer Index 7 (95% over 22bp)
AGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTA12.857142857142857No Hit
CTGTAGCCATCGGCTAGAGAGTTGGCTCCACCTCCGTAACGGGTGTAGTAA12.857142857142857No Hit
CTTCTGAAAGCATTCATCGATCCAAAGAAGAACTTCCTCGCTCGTATGCAT12.857142857142857No Hit
GTCCAATCACATGCAAGGCAGTACCAGCAATGGCGTGTTCAAGGCCCTGTG12.857142857142857No Hit
AGCTTCTTTAAGCCCTGAAAGTAACAAATTCTCCATCCTTTCTTCCTCTGG12.857142857142857No Hit
TATTGTAACAGTTGTAGACAAGGATCCTCGTATGATTCCAAGACAATTGTT12.857142857142857No Hit
CTGCTTTGCCGTACAAGCGTTCATCTCCGAGCTGGCTCAAGACAACCCCTC12.857142857142857No Hit
AGATACGATTTTCGCTGTTAACGAACAGTTAACTAATGCTAAGAAGCAAGG12.857142857142857No Hit
ACACTAATCAAAGCAAACAAACATGAAAACCACGAACACGAAACAAATATT12.857142857142857No Hit
GGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGCAGC12.857142857142857No Hit
GTTCCTCGGGTACGTGTTTATGAAACGGATCTACTTGACCCGAAGAGTGTA12.857142857142857No Hit
CTTTTCCATTTCGGAAAGCTCAATGATTTTCAGTAACAATGAAACCAAAAA12.857142857142857No Hit
CCATTTGAGCTATGAGACTTCATAGTCATTTTATCAACTTCATCAATGTAC12.857142857142857No Hit
AACGACTCTGGTTCAGAGATCTGAGTCTTGTGCTCAAACTCAGACGCGAGA12.857142857142857No Hit
GAAGAAAAGCTGTTATGTATCACTATTATTCTTACACACATCAAAGAGTCA12.857142857142857No Hit
AGCGCCTCCATCAGCACAGAGACGAAGTTTTGCATGTTCCCAGAGAAGAGG12.857142857142857No Hit
CCCTGATCCACCGCTAATATCGAACCCGATTCGAATTTTCCCACTTCCTAA12.857142857142857No Hit
GGGTAATTGCATCAGATGAACATACAAAATTATAATCACCATCTAAGCCTA12.857142857142857No Hit
TTGAAGGACCTCTTCATCTGCTTCTGCTTTTGTGGCGAATGCAGTGTGACC12.857142857142857No Hit
GTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCG12.857142857142857No Hit
CATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGC12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)