FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_83.3410000000ab20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_83.3410000000ab20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAAGGAAGGGTTAATGATTGCAAAGCTCCTGTCTCTTATACACATCTC12.5No Hit
GTTTGTTCTTCTTCGCTTTCCTTGGATTAAGAACAATGCTGATACTAGAAA12.5No Hit
ATTCTGATGTTAGCGAGCCTGAATCGGAGGATAATAAACAACCACTGCGAA12.5No Hit
TATCCGATCTTGGTTGGCCTTTCACCCCTAGCCACAAGTCATCCCCGTATT12.5No Hit
GTGGAGATCAAGCCTGAGATGATTGGACATTACCTTGCAGAGTTCTCCATC12.5No Hit
CAACAATCTCCTTCATCGTTGGTCTCTTAACCGGAGATCTTTGCAGACAAA12.5No Hit
ACATAATACCCAAGCATTTAACTTAAAGACTAGCTAACATTGATTAAAATT12.5No Hit
GTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTT12.5No Hit
TCTACAAAGTGGTGGAGATGGGTTCTTGAATAGGTTGATGAATTGATTGAT12.5No Hit
GCTTTAAGATCTAGCTTGCAGTCTAAGTTCACCGTGGAGACAATGTTTTGA12.5No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC12.5No Hit
TTTCCACTCCCTATCATTCGAGATCTGCTTCTTAGCTTAGGCCTTTGACAT12.5No Hit
ATCAATGAAGGGAACGAACCATGCTTCTGCACCCCTTACATTTCATAGGCG12.5No Hit
ATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT12.5No Hit
GTTATTAGGATTCGCTAGGTTAAACTCTTTTCGGAAATCCTCAAGGAAGAC12.5No Hit
GCATAAGGGAATGTGTCCAAACTAATATCCATCAAGGAATAGGCTCTGTCT12.5No Hit
GTATACTAAGAGCCATAAGAAGCTGAGGAAGGATGTTGAGAGTAAGAACTG12.5No Hit
CATATAACCTCTTCCTTTTCAAACACTGTATAGCCGCTGTCTCTTATACAC12.5No Hit
CATGTATACCTACGTAACAAACCTGCATATTCAGCACATTCAGTTCCTGTC12.5No Hit
GAACACAAGAGGTACAACTTGAAGAAACTGACTTACGAAGAGAGGAAGCTG12.5No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG12.5No Hit
GTGACAGACTTGGAGAGAAACCAAACAAAAACCTCCCAAAAATCCCCAATA12.5No Hit
GGGGTGTTATTGCATATCATCAAGGTTTCCAAACGGGAATATTGGACGTTT12.5No Hit
ACACATACTGGTTATAATCTTTCACTATTCTCATGATCTGTCTCTTATACA12.5No Hit
CTTGAGATTAGCCTTACTCATCTGTGCAGCTTCTCCTGTTGAAATCCCTAC12.5No Hit
GACAAGAAGGGAAAGGGAACATCTCTCTTTTAAGTTGTTGTTTCATCTATC12.5No Hit
GACAAGGGGAATCCTACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCT12.5No Hit
CCCAACATTTATGAGATCCTCAAGAACAAAAACTTACGGCTGTCTCTTATA12.5No Hit
CTTTAAAAGTCCACCAGTTCTTCAGGAAGTTGGGTATACTGATGCCGATGT12.5No Hit
GCCTTGGAGTACTTGGGGGTAGTGGCATCCATCTTGTTACAACAGCAGATC12.5No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT12.5No Hit
CTCCTGGTCTGAATCTTACACATCTAAAAGCAAGCTCAATGAACGCTTCCA12.5No Hit
TCCATATTCTGTATCTCCAGGATGTGAACTCGAAGGTATAAGCAAAGGATT12.5No Hit
GGTATAAGGCCGGAACAAGGGATTTTGGTGGCCATCGAAGATAAATTGCTG12.5No Hit
GTTAAACCATCTGCAATGCTTAAAGAGGTTGGAGATGTCATAGCTCCTAAC12.5No Hit
AGTCGATCTCTTTCAGTAAGAGCTTTCCTGTGTGCCAACCATTGGGTTCTC12.5No Hit
ACATCATTCCCATAACCACTTTCATCACCATAATCTTCCACATTTCTTTTT12.5No Hit
GGGGGAATGTATTGGAGTAGCATTCCAGGTTTACAGATCCGAAGAAACTGA12.5No Hit
GTTGATTGTTTGCAAGGATTCCTAATAAACCATTGTTTCATTGCAAAGTAA12.5No Hit
TGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGCC12.5No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)