##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_83.3410000000ab20.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.575 0.0 0.0 0.0 0.0 0.0 2 37.575 0.0 0.0 0.0 0.0 0.0 3 38.0 0.0 0.0 0.0 0.0 0.0 4 37.85 0.0 0.0 0.0 0.0 0.0 5 37.7 0.0 0.0 0.0 0.0 0.0 6 39.75 0.0 0.0 0.0 0.0 0.0 7 39.9 0.0 0.0 0.0 0.0 0.0 8 39.6 0.0 0.0 0.0 0.0 0.0 9 39.8 0.0 0.0 0.0 0.0 0.0 10 39.8 0.0 0.0 0.0 0.0 0.0 11 39.9 0.0 0.0 0.0 0.0 0.0 12 39.8 0.0 0.0 0.0 0.0 0.0 13 39.7 0.0 0.0 0.0 0.0 0.0 14 39.7 0.0 0.0 0.0 0.0 0.0 15 39.85 0.0 0.0 0.0 0.0 0.0 16 39.7 0.0 0.0 0.0 0.0 0.0 17 39.55 0.0 0.0 0.0 0.0 0.0 18 39.75 0.0 0.0 0.0 0.0 0.0 19 39.65 0.0 0.0 0.0 0.0 0.0 20 39.55 0.0 0.0 0.0 0.0 0.0 21 39.6 0.0 0.0 0.0 0.0 0.0 22 39.65 0.0 0.0 0.0 0.0 0.0 23 39.7 0.0 0.0 0.0 0.0 0.0 24 39.85 0.0 0.0 0.0 0.0 0.0 25 39.325 0.0 0.0 0.0 0.0 0.0 26 38.975 0.0 0.0 0.0 0.0 0.0 27 39.025 0.0 0.0 0.0 0.0 0.0 28 39.375 0.0 0.0 0.0 0.0 0.0 29 39.125 0.0 0.0 0.0 0.0 0.0 30 39.025 0.0 0.0 0.0 0.0 0.0 31 39.7 0.0 0.0 0.0 0.0 0.0 32 39.75 0.0 0.0 0.0 0.0 0.0 33 38.975 0.0 0.0 0.0 0.0 0.0 34 39.125 0.0 0.0 0.0 0.0 0.0 35 39.525 0.0 0.0 0.0 0.0 0.0 36 39.25 0.0 0.0 0.0 0.0 0.0 37 39.075 0.0 0.0 0.0 0.0 0.0 38 39.475 0.0 0.0 0.0 0.0 0.0 39 39.475 0.0 0.0 0.0 0.0 0.0 40 39.125 0.0 0.0 0.0 0.0 0.0 41 38.925 0.0 0.0 0.0 0.0 0.0 42 39.475 0.0 0.0 0.0 0.0 0.0 43 39.475 0.0 0.0 0.0 0.0 0.0 44 38.9 0.0 0.0 0.0 0.0 0.0 45 39.125 0.0 0.0 0.0 0.0 0.0 46 39.275 0.0 0.0 0.0 0.0 0.0 47 39.275 0.0 0.0 0.0 0.0 0.0 48 39.125 0.0 0.0 0.0 0.0 0.0 49 38.875 0.0 0.0 0.0 0.0 0.0 50 39.125 0.0 0.0 0.0 0.0 0.0 51 39.025 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.0 2201 2 0.0 2201 3 0.0 2201 4 0.0 2201 5 0.0 2201 6 0.5 2201 7 0.0 2201 8 0.0 2201 9 0.0 2201 10 0.0 2201 11 0.0 2201 12 0.0 2201 13 0.0 2201 14 0.0 2201 15 0.0 2201 16 0.0 2201 17 0.0 2201 18 0.0 2201 19 0.0 2201 20 0.0 2201 21 0.0 2201 22 0.0 2201 23 0.5 2201 24 0.0 2201 25 0.0 2201 26 0.5 2201 27 0.0 2201 28 0.0 2201 29 0.0 2201 30 0.0 2201 31 0.0 2201 32 0.0 2201 33 0.5 2201 34 0.5 2201 35 0.0 2201 36 0.0 2201 37 0.0 2201 38 0.0 2201 39 0.0 2201 40 0.0 2201 41 0.5 2201 42 0.0 2201 43 0.0 2201 44 0.5 2201 45 0.5 2201 46 0.0 2201 47 0.0 2201 48 0.5 2201 49 0.5 2201 50 0.5 2201 51 0.0 1112 1 0.0 1112 2 0.0 1112 3 0.0 1112 4 0.0 1112 5 0.0 1112 6 -1.5 1112 7 0.0 1112 8 0.0 1112 9 0.0 1112 10 0.0 1112 11 0.0 1112 12 0.0 1112 13 0.0 1112 14 0.0 1112 15 0.0 1112 16 0.0 1112 17 0.0 1112 18 0.0 1112 19 0.0 1112 20 0.0 1112 21 0.0 1112 22 0.0 1112 23 -1.5 1112 24 0.0 1112 25 0.0 1112 26 0.5 1112 27 0.0 1112 28 0.0 1112 29 0.0 1112 30 0.0 1112 31 0.0 1112 32 0.0 1112 33 0.5 1112 34 0.5 1112 35 0.0 1112 36 0.0 1112 37 0.0 1112 38 0.0 1112 39 0.0 1112 40 0.0 1112 41 0.5 1112 42 0.0 1112 43 0.0 1112 44 0.5 1112 45 0.5 1112 46 0.0 1112 47 0.0 1112 48 0.5 1112 49 0.5 1112 50 0.5 1112 51 0.0 2107 1 0.0 2107 2 0.0 2107 3 0.0 2107 4 0.0 2107 5 0.0 2107 6 0.5 2107 7 0.0 2107 8 0.0 2107 9 0.0 2107 10 0.0 2107 11 0.0 2107 12 0.0 2107 13 0.0 2107 14 0.0 2107 15 0.0 2107 16 0.0 2107 17 0.0 2107 18 0.0 2107 19 0.0 2107 20 0.0 2107 21 0.0 2107 22 0.0 2107 23 0.5 2107 24 0.0 2107 25 0.0 2107 26 -1.5 2107 27 0.0 2107 28 0.0 2107 29 0.0 2107 30 0.0 2107 31 0.0 2107 32 0.0 2107 33 -1.5 2107 34 -1.5 2107 35 0.0 2107 36 0.0 2107 37 0.0 2107 38 0.0 2107 39 0.0 2107 40 0.0 2107 41 -1.5 2107 42 0.0 2107 43 0.0 2107 44 0.5 2107 45 0.5 2107 46 0.0 2107 47 0.0 2107 48 -1.5 2107 49 0.5 2107 50 0.5 2107 51 0.0 2212 1 0.0 2212 2 0.0 2212 3 0.0 2212 4 0.0 2212 5 0.0 2212 6 0.5 2212 7 0.0 2212 8 0.0 2212 9 0.0 2212 10 0.0 2212 11 0.0 2212 12 0.0 2212 13 0.0 2212 14 0.0 2212 15 0.0 2212 16 0.0 2212 17 0.0 2212 18 0.0 2212 19 0.0 2212 20 0.0 2212 21 0.0 2212 22 0.0 2212 23 0.5 2212 24 0.0 2212 25 0.0 2212 26 0.5 2212 27 0.0 2212 28 0.0 2212 29 0.0 2212 30 0.0 2212 31 0.0 2212 32 0.0 2212 33 0.5 2212 34 0.5 2212 35 0.0 2212 36 0.0 2212 37 0.0 2212 38 0.0 2212 39 0.0 2212 40 0.0 2212 41 0.5 2212 42 0.0 2212 43 0.0 2212 44 -1.5 2212 45 -1.5 2212 46 0.0 2212 47 0.0 2212 48 0.5 2212 49 -1.5 2212 50 -1.5 2212 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 27 1.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 3.0 39 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5 17.5 17.5 22.5 2 15.0 17.5 42.5 25.0 3 15.0 22.5 42.5 20.0 4 17.5 30.0 25.0 27.500000000000004 5 10.0 45.0 30.0 15.0 6 27.500000000000004 45.0 17.5 10.0 7 20.0 40.0 30.0 10.0 8 20.0 30.0 30.0 20.0 9 30.0 17.5 20.0 32.5 10 15.0 22.5 40.0 22.5 11 27.500000000000004 20.0 22.5 30.0 12 27.500000000000004 20.0 40.0 12.5 13 25.0 20.0 30.0 25.0 14 17.5 25.0 25.0 32.5 15 25.0 22.5 20.0 32.5 16 22.5 42.5 22.5 12.5 17 27.500000000000004 30.0 27.500000000000004 15.0 18 20.0 30.0 25.0 25.0 19 17.5 25.0 22.5 35.0 20 20.0 22.5 37.5 20.0 21 22.5 27.500000000000004 25.0 25.0 22 17.5 32.5 32.5 17.5 23 7.5 27.500000000000004 37.5 27.500000000000004 24 22.5 25.0 32.5 20.0 25 25.0 25.0 27.500000000000004 22.5 26 17.5 27.500000000000004 32.5 22.5 27 17.5 47.5 17.5 17.5 28 22.5 30.0 27.500000000000004 20.0 29 12.5 32.5 32.5 22.5 30 12.5 30.0 32.5 25.0 31 15.0 27.500000000000004 35.0 22.5 32 20.0 32.5 17.5 30.0 33 20.0 35.0 20.0 25.0 34 30.0 20.0 25.0 25.0 35 20.0 22.5 30.0 27.500000000000004 36 20.0 27.500000000000004 30.0 22.5 37 27.500000000000004 22.5 37.5 12.5 38 12.5 40.0 30.0 17.5 39 12.5 32.5 25.0 30.0 40 22.5 25.0 32.5 20.0 41 17.5 40.0 27.500000000000004 15.0 42 27.500000000000004 30.0 27.500000000000004 15.0 43 17.5 27.500000000000004 40.0 15.0 44 17.5 22.5 27.500000000000004 32.5 45 22.5 27.500000000000004 32.5 17.5 46 15.0 22.5 30.0 32.5 47 15.0 32.5 27.500000000000004 25.0 48 25.0 22.5 32.5 20.0 49 20.0 30.0 22.5 27.500000000000004 50 10.0 25.0 47.5 17.5 51 12.5 25.0 32.5 30.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 0.0 28 0.0 29 0.0 30 1.0 31 2.0 32 1.5 33 1.0 34 1.0 35 1.0 36 2.0 37 3.0 38 3.0 39 3.0 40 6.0 41 9.0 42 7.5 43 6.0 44 4.5 45 3.0 46 4.0 47 5.0 48 3.5 49 2.0 50 2.0 51 2.0 52 1.5 53 1.0 54 0.5 55 0.0 56 0.0 57 0.0 58 0.5 59 1.0 60 0.5 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 40.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTACAAGGAAGGGTTAATGATTGCAAAGCTCCTGTCTCTTATACACATCTC 1 2.5 No Hit GTTTGTTCTTCTTCGCTTTCCTTGGATTAAGAACAATGCTGATACTAGAAA 1 2.5 No Hit ATTCTGATGTTAGCGAGCCTGAATCGGAGGATAATAAACAACCACTGCGAA 1 2.5 No Hit TATCCGATCTTGGTTGGCCTTTCACCCCTAGCCACAAGTCATCCCCGTATT 1 2.5 No Hit GTGGAGATCAAGCCTGAGATGATTGGACATTACCTTGCAGAGTTCTCCATC 1 2.5 No Hit CAACAATCTCCTTCATCGTTGGTCTCTTAACCGGAGATCTTTGCAGACAAA 1 2.5 No Hit ACATAATACCCAAGCATTTAACTTAAAGACTAGCTAACATTGATTAAAATT 1 2.5 No Hit GTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTT 1 2.5 No Hit TCTACAAAGTGGTGGAGATGGGTTCTTGAATAGGTTGATGAATTGATTGAT 1 2.5 No Hit GCTTTAAGATCTAGCTTGCAGTCTAAGTTCACCGTGGAGACAATGTTTTGA 1 2.5 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 1 2.5 No Hit TTTCCACTCCCTATCATTCGAGATCTGCTTCTTAGCTTAGGCCTTTGACAT 1 2.5 No Hit ATCAATGAAGGGAACGAACCATGCTTCTGCACCCCTTACATTTCATAGGCG 1 2.5 No Hit ATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT 1 2.5 No Hit GTTATTAGGATTCGCTAGGTTAAACTCTTTTCGGAAATCCTCAAGGAAGAC 1 2.5 No Hit GCATAAGGGAATGTGTCCAAACTAATATCCATCAAGGAATAGGCTCTGTCT 1 2.5 No Hit GTATACTAAGAGCCATAAGAAGCTGAGGAAGGATGTTGAGAGTAAGAACTG 1 2.5 No Hit CATATAACCTCTTCCTTTTCAAACACTGTATAGCCGCTGTCTCTTATACAC 1 2.5 No Hit CATGTATACCTACGTAACAAACCTGCATATTCAGCACATTCAGTTCCTGTC 1 2.5 No Hit GAACACAAGAGGTACAACTTGAAGAAACTGACTTACGAAGAGAGGAAGCTG 1 2.5 No Hit TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG 1 2.5 No Hit GTGACAGACTTGGAGAGAAACCAAACAAAAACCTCCCAAAAATCCCCAATA 1 2.5 No Hit GGGGTGTTATTGCATATCATCAAGGTTTCCAAACGGGAATATTGGACGTTT 1 2.5 No Hit ACACATACTGGTTATAATCTTTCACTATTCTCATGATCTGTCTCTTATACA 1 2.5 No Hit CTTGAGATTAGCCTTACTCATCTGTGCAGCTTCTCCTGTTGAAATCCCTAC 1 2.5 No Hit GACAAGAAGGGAAAGGGAACATCTCTCTTTTAAGTTGTTGTTTCATCTATC 1 2.5 No Hit GACAAGGGGAATCCTACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCT 1 2.5 No Hit CCCAACATTTATGAGATCCTCAAGAACAAAAACTTACGGCTGTCTCTTATA 1 2.5 No Hit CTTTAAAAGTCCACCAGTTCTTCAGGAAGTTGGGTATACTGATGCCGATGT 1 2.5 No Hit GCCTTGGAGTACTTGGGGGTAGTGGCATCCATCTTGTTACAACAGCAGATC 1 2.5 No Hit TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT 1 2.5 No Hit CTCCTGGTCTGAATCTTACACATCTAAAAGCAAGCTCAATGAACGCTTCCA 1 2.5 No Hit TCCATATTCTGTATCTCCAGGATGTGAACTCGAAGGTATAAGCAAAGGATT 1 2.5 No Hit GGTATAAGGCCGGAACAAGGGATTTTGGTGGCCATCGAAGATAAATTGCTG 1 2.5 No Hit GTTAAACCATCTGCAATGCTTAAAGAGGTTGGAGATGTCATAGCTCCTAAC 1 2.5 No Hit AGTCGATCTCTTTCAGTAAGAGCTTTCCTGTGTGCCAACCATTGGGTTCTC 1 2.5 No Hit ACATCATTCCCATAACCACTTTCATCACCATAATCTTCCACATTTCTTTTT 1 2.5 No Hit GGGGGAATGTATTGGAGTAGCATTCCAGGTTTACAGATCCGAAGAAACTGA 1 2.5 No Hit GTTGATTGTTTGCAAGGATTCCTAATAAACCATTGTTTCATTGCAAAGTAA 1 2.5 No Hit TGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGCC 1 2.5 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 2.5 31 0.0 0.0 2.5 32 0.0 0.0 5.0 33 0.0 0.0 5.0 34 0.0 0.0 5.0 35 0.0 0.0 5.0 36 0.0 0.0 5.0 37 0.0 0.0 7.5 38 0.0 0.0 10.0 39 0.0 0.0 10.0 >>END_MODULE >>Kmer Content pass >>END_MODULE