##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_82.3410000000ab13.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.833333333333336 0.0 0.0 0.0 0.0 0.0 2 37.666666666666664 0.0 0.0 0.0 0.0 0.0 3 37.5 0.0 0.0 0.0 0.0 0.0 4 37.52777777777778 0.0 0.0 0.0 0.0 0.0 5 37.5 0.0 0.0 0.0 0.0 0.0 6 39.833333333333336 0.0 0.0 0.0 0.0 0.0 7 39.72222222222222 0.0 0.0 0.0 0.0 0.0 8 39.666666666666664 0.0 0.0 0.0 0.0 0.0 9 39.77777777777778 0.0 0.0 0.0 0.0 0.0 10 39.72222222222222 0.0 0.0 0.0 0.0 0.0 11 39.833333333333336 0.0 0.0 0.0 0.0 0.0 12 39.666666666666664 0.0 0.0 0.0 0.0 0.0 13 39.333333333333336 0.0 0.0 0.0 0.0 0.0 14 39.083333333333336 0.0 0.0 0.0 0.0 0.0 15 39.72222222222222 0.0 0.0 0.0 0.0 0.0 16 39.611111111111114 0.0 0.0 0.0 0.0 0.0 17 39.111111111111114 0.0 0.0 0.0 0.0 0.0 18 38.77777777777778 0.0 0.0 0.0 0.0 0.0 19 39.02777777777778 0.0 0.0 0.0 0.0 0.0 20 39.083333333333336 0.0 0.0 0.0 0.0 0.0 21 39.666666666666664 0.0 0.0 0.0 0.0 0.0 22 38.97222222222222 0.0 0.0 0.0 0.0 0.0 23 39.47222222222222 0.0 0.0 0.0 0.0 0.0 24 39.361111111111114 0.0 0.0 0.0 0.0 0.0 25 39.44444444444444 0.0 0.0 0.0 0.0 0.0 26 39.72222222222222 0.0 0.0 0.0 0.0 0.0 27 39.77777777777778 0.0 0.0 0.0 0.0 0.0 28 39.666666666666664 0.0 0.0 0.0 0.0 0.0 29 38.97222222222222 0.0 0.0 0.0 0.0 0.0 30 39.888888888888886 0.0 0.0 0.0 0.0 0.0 31 39.611111111111114 0.0 0.0 0.0 0.0 0.0 32 39.44444444444444 0.0 0.0 0.0 0.0 0.0 33 38.69444444444444 0.0 0.0 0.0 0.0 0.0 34 38.611111111111114 0.0 0.0 0.0 0.0 0.0 35 39.111111111111114 0.0 0.0 0.0 0.0 0.0 36 39.02777777777778 0.0 0.0 0.0 0.0 0.0 37 39.47222222222222 0.0 0.0 0.0 0.0 0.0 38 39.19444444444444 0.0 0.0 0.0 0.0 0.0 39 39.388888888888886 0.0 0.0 0.0 0.0 0.0 40 39.083333333333336 0.0 0.0 0.0 0.0 0.0 41 39.333333333333336 0.0 0.0 0.0 0.0 0.0 42 39.27777777777778 0.0 0.0 0.0 0.0 0.0 43 38.833333333333336 0.0 0.0 0.0 0.0 0.0 44 38.97222222222222 0.0 0.0 0.0 0.0 0.0 45 39.111111111111114 0.0 0.0 0.0 0.0 0.0 46 38.77777777777778 0.0 0.0 0.0 0.0 0.0 47 39.30555555555556 0.0 0.0 0.0 0.0 0.0 48 38.97222222222222 0.0 0.0 0.0 0.0 0.0 49 39.30555555555556 0.0 0.0 0.0 0.0 0.0 50 39.138888888888886 0.0 0.0 0.0 0.0 0.0 51 38.72222222222222 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2202 1 0.0 2202 2 0.0 2202 3 0.0 2202 4 0.0 2202 5 0.0 2202 6 0.0 2202 7 0.0 2202 8 0.0 2202 9 0.0 2202 10 0.0 2202 11 0.0 2202 12 0.6666666666666661 2202 13 -1.333333333333334 2202 14 0.0 2202 15 0.0 2202 16 0.0 2202 17 0.0 2202 18 0.0 2202 19 0.6666666666666661 2202 20 0.0 2202 21 0.0 2202 22 0.0 2202 23 0.0 2202 24 0.0 2202 25 0.0 2202 26 0.0 2202 27 0.0 2202 28 0.6666666666666661 2202 29 0.6666666666666661 2202 30 0.0 2202 31 0.0 2202 32 0.0 2202 33 0.0 2202 34 0.0 2202 35 0.0 2202 36 0.0 2202 37 0.0 2202 38 0.0 2202 39 0.0 2202 40 0.0 2202 41 0.0 2202 42 0.6666666666666661 2202 43 0.0 2202 44 0.0 2202 45 0.0 2202 46 0.0 2202 47 0.0 2202 48 0.0 2202 49 0.0 2202 50 0.0 2202 51 0.0 1113 1 0.0 1113 2 0.0 1113 3 0.0 1113 4 0.0 1113 5 0.0 1113 6 0.0 1113 7 0.0 1113 8 0.0 1113 9 0.0 1113 10 0.0 1113 11 0.0 1113 12 0.6666666666666661 1113 13 0.6666666666666661 1113 14 0.0 1113 15 0.0 1113 16 0.0 1113 17 0.0 1113 18 0.0 1113 19 -1.333333333333334 1113 20 0.0 1113 21 0.0 1113 22 0.0 1113 23 0.0 1113 24 0.0 1113 25 0.0 1113 26 0.0 1113 27 0.0 1113 28 0.6666666666666661 1113 29 0.6666666666666661 1113 30 0.0 1113 31 0.0 1113 32 0.0 1113 33 0.0 1113 34 0.0 1113 35 0.0 1113 36 0.0 1113 37 0.0 1113 38 0.0 1113 39 0.0 1113 40 0.0 1113 41 0.0 1113 42 0.6666666666666661 1113 43 0.0 1113 44 0.0 1113 45 0.0 1113 46 0.0 1113 47 0.0 1113 48 0.0 1113 49 0.0 1113 50 0.0 1113 51 0.0 2104 1 0.0 2104 2 0.0 2104 3 0.0 2104 4 0.0 2104 5 0.0 2104 6 0.0 2104 7 0.0 2104 8 0.0 2104 9 0.0 2104 10 0.0 2104 11 0.0 2104 12 -1.333333333333334 2104 13 0.6666666666666661 2104 14 0.0 2104 15 0.0 2104 16 0.0 2104 17 0.0 2104 18 0.0 2104 19 0.6666666666666661 2104 20 0.0 2104 21 0.0 2104 22 0.0 2104 23 0.0 2104 24 0.0 2104 25 0.0 2104 26 0.0 2104 27 0.0 2104 28 -1.333333333333334 2104 29 -1.333333333333334 2104 30 0.0 2104 31 0.0 2104 32 0.0 2104 33 0.0 2104 34 0.0 2104 35 0.0 2104 36 0.0 2104 37 0.0 2104 38 0.0 2104 39 0.0 2104 40 0.0 2104 41 0.0 2104 42 -1.333333333333334 2104 43 0.0 2104 44 0.0 2104 45 0.0 2104 46 0.0 2104 47 0.0 2104 48 0.0 2104 49 0.0 2104 50 0.0 2104 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 31 1.0 32 1.0 33 0.0 34 0.0 35 0.0 36 0.0 37 2.0 38 0.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44444444444444 11.11111111111111 16.666666666666664 27.77777777777778 2 13.88888888888889 19.444444444444446 38.88888888888889 27.77777777777778 3 11.11111111111111 27.77777777777778 38.88888888888889 22.22222222222222 4 5.555555555555555 19.444444444444446 19.444444444444446 55.55555555555556 5 16.666666666666664 25.0 36.11111111111111 22.22222222222222 6 27.77777777777778 38.88888888888889 22.22222222222222 11.11111111111111 7 27.77777777777778 38.88888888888889 27.77777777777778 5.555555555555555 8 41.66666666666667 27.77777777777778 8.333333333333332 22.22222222222222 9 25.0 11.11111111111111 30.555555555555557 33.33333333333333 10 11.11111111111111 19.444444444444446 36.11111111111111 33.33333333333333 11 22.22222222222222 27.77777777777778 27.77777777777778 22.22222222222222 12 25.0 27.77777777777778 25.0 22.22222222222222 13 30.555555555555557 27.77777777777778 27.77777777777778 13.88888888888889 14 11.11111111111111 22.22222222222222 30.555555555555557 36.11111111111111 15 19.444444444444446 38.88888888888889 19.444444444444446 22.22222222222222 16 38.88888888888889 22.22222222222222 16.666666666666664 22.22222222222222 17 27.77777777777778 19.444444444444446 22.22222222222222 30.555555555555557 18 27.77777777777778 41.66666666666667 16.666666666666664 13.88888888888889 19 25.0 38.88888888888889 16.666666666666664 19.444444444444446 20 50.0 27.77777777777778 13.88888888888889 8.333333333333332 21 27.77777777777778 36.11111111111111 19.444444444444446 16.666666666666664 22 30.555555555555557 25.0 30.555555555555557 13.88888888888889 23 19.444444444444446 36.11111111111111 30.555555555555557 13.88888888888889 24 22.22222222222222 33.33333333333333 13.88888888888889 30.555555555555557 25 25.0 30.555555555555557 19.444444444444446 25.0 26 11.11111111111111 33.33333333333333 36.11111111111111 19.444444444444446 27 13.88888888888889 47.22222222222222 25.0 13.88888888888889 28 25.0 36.11111111111111 22.22222222222222 16.666666666666664 29 11.11111111111111 30.555555555555557 22.22222222222222 36.11111111111111 30 8.333333333333332 38.88888888888889 22.22222222222222 30.555555555555557 31 27.77777777777778 25.0 22.22222222222222 25.0 32 30.555555555555557 22.22222222222222 22.22222222222222 25.0 33 30.555555555555557 25.0 19.444444444444446 25.0 34 27.77777777777778 22.22222222222222 22.22222222222222 27.77777777777778 35 22.22222222222222 22.22222222222222 27.77777777777778 27.77777777777778 36 8.333333333333332 38.88888888888889 30.555555555555557 22.22222222222222 37 25.0 33.33333333333333 13.88888888888889 27.77777777777778 38 22.22222222222222 36.11111111111111 25.0 16.666666666666664 39 30.555555555555557 25.0 19.444444444444446 25.0 40 25.0 27.77777777777778 27.77777777777778 19.444444444444446 41 30.555555555555557 16.666666666666664 30.555555555555557 22.22222222222222 42 13.88888888888889 30.555555555555557 33.33333333333333 22.22222222222222 43 19.444444444444446 19.444444444444446 33.33333333333333 27.77777777777778 44 25.0 25.0 25.0 25.0 45 25.0 27.77777777777778 30.555555555555557 16.666666666666664 46 25.0 30.555555555555557 16.666666666666664 27.77777777777778 47 16.666666666666664 22.22222222222222 25.0 36.11111111111111 48 8.333333333333332 19.444444444444446 30.555555555555557 41.66666666666667 49 27.77777777777778 8.333333333333332 38.88888888888889 25.0 50 25.0 19.444444444444446 38.88888888888889 16.666666666666664 51 16.666666666666664 22.22222222222222 38.88888888888889 22.22222222222222 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.5 31 1.0 32 2.0 33 3.0 34 1.5 35 0.0 36 1.0 37 2.0 38 3.5 39 5.0 40 3.0 41 1.0 42 2.5 43 4.0 44 3.5 45 3.0 46 6.0 47 9.0 48 4.5 49 0.0 50 0.0 51 0.0 52 0.5 53 1.0 54 0.5 55 0.0 56 0.5 57 1.0 58 1.0 59 1.0 60 1.0 61 1.0 62 0.5 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.5 69 1.0 70 1.0 71 1.0 72 1.0 73 1.0 74 1.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 36.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTC 1 2.7777777777777777 No Hit ACCACAAGTACCGTGTGAAGAGAAACTGCTGGCCTAAGGTTCGTGGTGTGG 1 2.7777777777777777 No Hit TTTTTTTTTTTTTTGCGTTGATACCACTCCCGAACCCTGCGTTGATACTGT 1 2.7777777777777777 No Hit GTCCTAAGGAAGGTTTTTGGTTTAGTCATCTTCTTTGGATCTATGACTCTG 1 2.7777777777777777 No Hit CTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT 1 2.7777777777777777 No Hit TGACCCAACCGGTTTTCATAGCTCCAGAACTTGCAAAATAGTTCCAGTGAC 1 2.7777777777777777 No Hit GGACAAGCTCATAAAGGAAGGTGAGTATCAGTTACTGAAGAAGCTGTCTCT 1 2.7777777777777777 No Hit GGATTATGTTGCAAAGCAAGGTCAATAGGCAGTCTATTCCTGTCTCTTATA 1 2.7777777777777777 No Hit GAGAAGGCACCAGCGGCAGAAGAAACAACATCTGTTAAGGAAGACAAGTGA 1 2.7777777777777777 No Hit GCCAAGGACAAGAAGGAGAAGGAGAAAAAAGGATAAGGAGGAAAAGGAGAA 1 2.7777777777777777 No Hit CCTGCTGGGCGCGCCGATCGCGCGCTTCGCGGCGTTGGTGCTCGTCCTCGT 1 2.7777777777777777 No Hit TTCTCTTTCTTGTACCAAAAGCTTTTTGAATCTCCAAGATCTGTCTCTTAT 1 2.7777777777777777 No Hit GTGTTGGATTTGTATCAAAGGAAATGATTCAGACTCATTGCCCTGCACCTG 1 2.7777777777777777 No Hit TATCAACGCAGAGTACGGGGAGAGTAACCAACAACCCAAAGCGATCAAAGA 1 2.7777777777777777 No Hit AAACAGGGGTTGTACCCCGCCTCCGATTCACGGAGTAAGTAAAATAACGTT 1 2.7777777777777777 No Hit GAGAAATGTCTATTCATGATATTCATGTCCTTTGACCACTTTTGGATGGGG 1 2.7777777777777777 No Hit CTTCTTTGGAGATCCACATTTGTTGGAAAGTAGACAAAGAGGCCAAGATAG 1 2.7777777777777777 No Hit CTTCAAAGTGTTTGCAGAGAAATCTAAATTCATCAACAGTTTTTTACCTGT 1 2.7777777777777777 No Hit ATCCTAAAGCACACAACAACTGAAAAAACAAACACCTTTCTATCTTCTTTG 1 2.7777777777777777 No Hit GGACTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGACCGGTTAGTGACTT 1 2.7777777777777777 No Hit GCTCCTGAGTTCATAGAGATGAGTGTTGAGCAGGAGCTGTCTCTTATACAC 1 2.7777777777777777 No Hit GCACTAGCTTTAACATTAAACGATACAAAAACAGCAGTGAACAGGCTTCAT 1 2.7777777777777777 No Hit CCTACAACATCAAGTGTTCAAAACCCATCAGTGGTAGATTTAGGTGCTGTC 1 2.7777777777777777 No Hit GCATTTGCTTGTCTAGAAAGGATTTTATTTCTCCTTCGCTTGTGAAGCTTA 1 2.7777777777777777 No Hit GTACGTAAGGTTACAACGTGAATACCAGCAGCAGCATCAGTCCTGTCTCTT 1 2.7777777777777777 No Hit CCTGGGAAGCGAAAAGGGAGATAACTTCAAGGGGAATAAAGACTGTCTCTT 1 2.7777777777777777 No Hit GGTATCAACGCAGAGTACGGGAAAGTCAACGAGCTTAACCTTAAGGACACA 1 2.7777777777777777 No Hit CTTAAATACCAAGGAGCGATTGACAACCGCAAGTGGCAAATGTCAATCGGC 1 2.7777777777777777 No Hit GTCCACGCCTCCTTACGGTAACGCTTCAACCCATGCACGACGCTCCCCTAC 1 2.7777777777777777 No Hit TACCTATCCTCCTTTGCACCATACCTATCATCTTTTGCACCATACCCTGTC 1 2.7777777777777777 No Hit TCTCTAATCAAAACAAGAAAGACACAAAGATGGAGATCTCAAAAACCCTTT 1 2.7777777777777777 No Hit CAACCGCACCGGGCTGGCGAAGGGCAAGACGCCGCTGGCGGTGGAGAAGCA 1 2.7777777777777777 No Hit CTTCTATGTGATGCTCCTCGTCTCGGTCTTCTCTGTCATAGAACTGTCTCT 1 2.7777777777777777 No Hit CTTTCGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTCTGTCTCT 1 2.7777777777777777 No Hit GAACGGAGCAATCCGGTCCGCCGATCGATTCGGGGCGTGGACCGACGCGGA 1 2.7777777777777777 No Hit GTCCGCTGACAGCCGACGGGTTCGGAACTGGGACCCCCGAGCCCAGCCCTC 1 2.7777777777777777 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 2.7777777777777777 34 0.0 0.0 2.7777777777777777 35 0.0 0.0 2.7777777777777777 36 0.0 0.0 2.7777777777777777 37 0.0 0.0 5.555555555555555 38 0.0 0.0 5.555555555555555 39 0.0 0.0 5.555555555555555 >>END_MODULE >>Kmer Content pass >>END_MODULE