FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_82.3410000000ab13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_82.3410000000ab13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTC12.7777777777777777No Hit
ACCACAAGTACCGTGTGAAGAGAAACTGCTGGCCTAAGGTTCGTGGTGTGG12.7777777777777777No Hit
TTTTTTTTTTTTTTGCGTTGATACCACTCCCGAACCCTGCGTTGATACTGT12.7777777777777777No Hit
GTCCTAAGGAAGGTTTTTGGTTTAGTCATCTTCTTTGGATCTATGACTCTG12.7777777777777777No Hit
CTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT12.7777777777777777No Hit
TGACCCAACCGGTTTTCATAGCTCCAGAACTTGCAAAATAGTTCCAGTGAC12.7777777777777777No Hit
GGACAAGCTCATAAAGGAAGGTGAGTATCAGTTACTGAAGAAGCTGTCTCT12.7777777777777777No Hit
GGATTATGTTGCAAAGCAAGGTCAATAGGCAGTCTATTCCTGTCTCTTATA12.7777777777777777No Hit
GAGAAGGCACCAGCGGCAGAAGAAACAACATCTGTTAAGGAAGACAAGTGA12.7777777777777777No Hit
GCCAAGGACAAGAAGGAGAAGGAGAAAAAAGGATAAGGAGGAAAAGGAGAA12.7777777777777777No Hit
CCTGCTGGGCGCGCCGATCGCGCGCTTCGCGGCGTTGGTGCTCGTCCTCGT12.7777777777777777No Hit
TTCTCTTTCTTGTACCAAAAGCTTTTTGAATCTCCAAGATCTGTCTCTTAT12.7777777777777777No Hit
GTGTTGGATTTGTATCAAAGGAAATGATTCAGACTCATTGCCCTGCACCTG12.7777777777777777No Hit
TATCAACGCAGAGTACGGGGAGAGTAACCAACAACCCAAAGCGATCAAAGA12.7777777777777777No Hit
AAACAGGGGTTGTACCCCGCCTCCGATTCACGGAGTAAGTAAAATAACGTT12.7777777777777777No Hit
GAGAAATGTCTATTCATGATATTCATGTCCTTTGACCACTTTTGGATGGGG12.7777777777777777No Hit
CTTCTTTGGAGATCCACATTTGTTGGAAAGTAGACAAAGAGGCCAAGATAG12.7777777777777777No Hit
CTTCAAAGTGTTTGCAGAGAAATCTAAATTCATCAACAGTTTTTTACCTGT12.7777777777777777No Hit
ATCCTAAAGCACACAACAACTGAAAAAACAAACACCTTTCTATCTTCTTTG12.7777777777777777No Hit
GGACTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGACCGGTTAGTGACTT12.7777777777777777No Hit
GCTCCTGAGTTCATAGAGATGAGTGTTGAGCAGGAGCTGTCTCTTATACAC12.7777777777777777No Hit
GCACTAGCTTTAACATTAAACGATACAAAAACAGCAGTGAACAGGCTTCAT12.7777777777777777No Hit
CCTACAACATCAAGTGTTCAAAACCCATCAGTGGTAGATTTAGGTGCTGTC12.7777777777777777No Hit
GCATTTGCTTGTCTAGAAAGGATTTTATTTCTCCTTCGCTTGTGAAGCTTA12.7777777777777777No Hit
GTACGTAAGGTTACAACGTGAATACCAGCAGCAGCATCAGTCCTGTCTCTT12.7777777777777777No Hit
CCTGGGAAGCGAAAAGGGAGATAACTTCAAGGGGAATAAAGACTGTCTCTT12.7777777777777777No Hit
GGTATCAACGCAGAGTACGGGAAAGTCAACGAGCTTAACCTTAAGGACACA12.7777777777777777No Hit
CTTAAATACCAAGGAGCGATTGACAACCGCAAGTGGCAAATGTCAATCGGC12.7777777777777777No Hit
GTCCACGCCTCCTTACGGTAACGCTTCAACCCATGCACGACGCTCCCCTAC12.7777777777777777No Hit
TACCTATCCTCCTTTGCACCATACCTATCATCTTTTGCACCATACCCTGTC12.7777777777777777No Hit
TCTCTAATCAAAACAAGAAAGACACAAAGATGGAGATCTCAAAAACCCTTT12.7777777777777777No Hit
CAACCGCACCGGGCTGGCGAAGGGCAAGACGCCGCTGGCGGTGGAGAAGCA12.7777777777777777No Hit
CTTCTATGTGATGCTCCTCGTCTCGGTCTTCTCTGTCATAGAACTGTCTCT12.7777777777777777No Hit
CTTTCGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTCTGTCTCT12.7777777777777777No Hit
GAACGGAGCAATCCGGTCCGCCGATCGATTCGGGGCGTGGACCGACGCGGA12.7777777777777777No Hit
GTCCGCTGACAGCCGACGGGTTCGGAACTGGGACCCCCGAGCCCAGCCCTC12.7777777777777777No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)